易锦生物提供的所有人、小鼠和大鼠ORF 克隆及其慢病毒、AAV 颗粒与重组蛋白均使用基于毛细管电泳的 Sanger 法“金标准”全长测序,保证氨基酸序列与NCBI 数据库一致。 All human and mice ORF clones and related Lentivirus, AAV particles and recombinant proteins provided by iGeneBio are fully sequenced using Sanger sequencing by capillary electrophoresis and Amino Acid Sequences are guaranteed to be matched with NCBI database.
Anti-GST (glutathione-S-transferase) tag antibody is a purified mouse IgG2a monoclonal antibody that binds to GST-fusion proteins. Anti-GST tag antibody can be used to identify and capture fusion proteins by western blots and immunoprecipita-tion. Immobilized anti-GST is also used for affinity purification of GST fusion proteins. OmicsLink™ anti-tag antibodies are ideal for use with OmicsLink™ expression-ready ORF cDNA clones.
The OmicsLink™ anti-GST tag antibody was produced by immunizing mice with a synthetic peptide of Schistosoma japonicum GST.
Specificity
The anti-GST tag monoclonal antibody detects over-expressed GST fusion proteins.
Figure 1. 20 ng of recombinant GST protein (lane 1) and 20 µg of 293 cell lysates were transfected with a GST expression vector and probed with OmicsLink Anti-GST (1 µg/ml).
Formulation
0.25 mg/ml in PBS, pH 7.4 with 0.05% sodium azide and 50% (v/v) glycerol.
Applications
Western blot, immunoprecipitation, immunostaining and affinity purification.
Storage/Stability
Stable for 1 year at -20°C from shipment date. Briefly centrifuge the original vial prior to opening the cap to collect the product at the bottom of the tube.
Starting dilution recommendations
Western blot at 1:1000
immunoprecipitation and immunostaining at 1:200