• 易锦生物提供的所有人、小鼠和大鼠ORF 克隆及其慢病毒、AAV 颗粒与重组蛋白均使用基于毛细管电泳的 Sanger 法“金标准”全长测序,保证氨基酸序列与NCBI 数据库一致。
    All human and mice ORF clones and related Lentivirus, AAV particles and recombinant proteins provided by iGeneBio are fully sequenced using Sanger sequencing by capillary electrophoresis and Amino Acid Sequences are guaranteed to be matched with NCBI database.
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抗proBDNF抗体

GeneCopoeia’s 提供的抗人前脑源性神经营养因子(Human proBDNF)抗体是由小鼠单克隆抗体制得。本抗体对人前脑源性神经营养因子(proBDNF)高度特异,不识别成熟的BDNF。

免疫原

纯化的GST-前区蛋白可用于制备免疫小鼠及筛选杂交瘤克隆。mAb287克隆从初期筛选获得,并使用相关的重组蛋白、神经元、脑细胞溶解产物及来自bdnf KO或野生型同窝仔畜(如下方数据所示)的切片作进一步鉴定。

特异性

本抗proBDNF抗体只识别人的前BDNF,不识别成熟的BDNF。

产品应用

Western blot、免疫共沉淀、免疫染色及亲和纯化。

pro-bdnf-antibody
Figure 1. 对抗前体抗体的鉴定. (a) Western blot蛋白印迹鉴定mAb287单克隆抗体的免疫特异性。运用SDSPAGE及带有抗成熟BDNF抗体。(N-20, Santa Cruz) (左泳道所示)的探针,及带有mAb287单克隆抗体的探针(右泳道所示),将纯化的重组成熟BDNF(50 ng)和纯化的重组proBDNF(50 ng)分离。(b) 使用mAb287抗体对脑细胞溶解产物中的proBDNF进行检测。bdnf -/-、bdnf +/+ 和 bdnf-HA/HA 小鼠的海马体细胞溶解产物与mAb287进行免疫共沉淀。使用SDS-PAGE分离条带后,使用前文的抗mAb287、抗HA.11或抗成熟BDNF抗体对其进行免疫共沉淀。缺失的前体条带如图所标示,和理论条带大小一致,为14至16 kDa。

产品订购

购买 货号 产品 描述 价格(¥)
AT021 Anti-Human proBDNF Antibody Mouse monoclonal antibody specific for the prodomain of human brain-derived neurotrophic factor (proBDNF). 2700

已发表的文章

Wang, B.D., et al. (2022). miR-31 from Mesenchymal Stem Cell-Derived Extracellular Vesicles Alleviates Intervertebral Disc Degeneration by Inhibiting NFAT5 and Upregulating the Wnt/β-Catenin Pathway. Stem Cells Int doi: 10.1155/2022/2164057 [anti-NC; GL–02]

Smulders, L., et al. (2022). Phosphatidylinositol Monophosphates Regulate the Membrane Localization of HSPA1A, a Stress-Inducible 70-kDa Heat Shock Protein. Biomolecules  doi: 10.3390/biom12060856 [OmicsLink™ monoclonal mouse anti-GFP antibody; OmicsLink™ anti-c-Myc antibody]

Mizui, T., et al. (2019). Cerebrospinal fluid BDNF pro-peptide levels in major depressive disorder and schizophrenia. Journal of Psychiatric Research doi: 10.1016/j.jpsychires.2019.03.024 [anti-human proBDNF antibody]

Benicky, J., et a.. (2019).N-Glycosylation is required for secretion of the precursor to brain-derived neurotrophic factor (proBDNF) carrying sulfated LacdiNAc structures. The Journal of Biological Chemistry doi: 10.1074/jbc.RA119.009989 [anti-human proBDNF antibody]

Yang, B., et al. (2017). Altered expression of BDNF, BDNF pro-peptide and their precursor proBDNF in brain and liver tissues from psychiatric disorders: rethinking the brain–liver axis. Translational Psychiatry doi: 10.1038/tp.2017.95 [anti-human proBDNF antibody]

Zhang, S., et al. (2017). Inhibition of BDNF production by MPP+ through up-regulation of miR-210-3p contributes to dopaminergic neuron damage in MPTP model. Neuorscience Letters doi: 10.1016/j.neulet.2017.10.014 [anti-human proBDNF antibody]

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