Applied BioProbes has developed a breakthrough antibody labeling technology with Andy Fluor™ dyes — iLink™ Antibody Labeling Kits. With this technology, you only need to mix your antibody with Andy Fluor™ dye provided in the kit, and in 30 minutes you will have an optimally labeled Andy Fluor™ dye-antibody conjugate ready for immunostaining. The labeling technology provides unprecedented convenience for multicolor immunostaining, where pre-labeled primary antibodies may not be available, and indirect staining via pre-labeled fluorescent secondary antibodies may also be difficult due to cross-interaction among different antibodies.
iLink™ Antibody Labeling Kit Selection Guide
|Fluorophore||Ex/Em (nm)||Imaging||Flow cytometry||Cat. No.|
|Andy Fluor™ 350||350/440||✔||L022|
|Andy Fluor™ 405M||405/450||✔||✔||L023|
|Andy Fluor™ 488||495/520||✔||✔||L024|
|Andy Fluor™ 555||553/565||✔||L025|
|Andy Fluor™ 568||578/602||✔||L026|
|Andy Fluor™ 594||590/615||✔||L027|
|Andy Fluor™ 647||650/666||✔||✔||L028|
How iLink™ Antibody Labeling Kits work
The Antibody Labeling Kits utilize an amine-reactive Andy Fluor™ fluorophore to covalently attach the label to the IgG antibody of interest. The Antibody Labeling Kits are optimized for labeling 50-100 µg of antibody per reaction, and contain everything you need to perform the conjugation. Simply mix your antibody with a vial of pre-measured Andy Fluor™ dye, biotin, or FITC, and wait for 30 minutes, you will have an optimally labeled antibody conjugate ready to be used in any immunofluorescence staining experiment.
Figure 1. Label your antibody in 30 minutes.