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核酸标记探针

产品介绍

GeneCopoeia 最新推出一系列荧光素和生物素标记dUTP和UTP探针。这些探针可广泛应用于FISH, TUNEL, DNA microarray等应用。我们的探针采用荧光亮度强,光稳定性好的Andy Fluor染料标记,并且在染料和dUTP及UTP之间设计一条特殊的X-linker以确保在酶的作用下更有效的插入DNA或RNA。

产品特点

◆荧光信号强,稳定性好,背景低;
◆多种颜色可供选择,适合多重染色;
◆适用性广:适用于各种酶的标记。​

 

Figure 1. Fluorescence in situ Hybridization (FISH) of CEP7/MET probes labeled with Andy Fluor dyes. Human Chromosome 7 Enumeration (CEP7) and Human c-Met gene (MET) probes were labeled with Andy Fluor 488-X-dUTP (green) and Andy Fluor 568-X-dUTP (magenta), respectively, using nick translation. The labeled probes were mixed together in hybridization buffers with blocking DNA, and incubated with HT1080 cells on slides at 37°C overnight. The slides was washed and mounted with AntiFade solution containing DAPI (blue), then visualized with an Epi-Fluorescence microscope.

TUNEL-biotin-11-UTP mouse tongue

Figure 2. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL)-mediated detection of apoptotic cells in mouse tongue tissue using Biotin-11-dUTP. Left: Cells were labeled with Biotin-11-dUTP, followed by detection with AndyFluor™ 488-streptavidin (green). Nuclei were also counterstained with DAPI (blue). Right: Cells were labeled with Biotin-11-dUTP, followed by detection with horse radish peroxidase (HRP).