产品介绍
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miRNA抑制剂可以特异、高效地抑制生物体内源性miRNA的活性，是研究miRNA功能缺失的重要工具。GeneCopoeia提供慢病毒或非病毒载体的miArrest™ miRNA抑制剂表达克隆和化学合成的miRNA抑制剂。 |
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miArrest™ miRNA抑制剂克隆可以特异性地结合目的miRNA，瞬时和稳定地抑制miRNA的功能，采用H1或者U6启动子驱动转录可以保证在大多数哺乳动物细胞中高水平表达。 |
优势
覆盖面广
- 针对所有已知的人、小鼠和大鼠miRNA
灵活的载体选择
- 慢病毒载体可转导普通细胞和难转染、未分化细胞
- 非病毒载体可进行稳定或瞬时转导
先进可靠的设计
- 表达框经过专利算法设计并经过严格的实验验证
抑制性能优越
- 与其他化学合成的miRNA抑制剂相比具有更强、更持久的抑制作用和极低的细胞毒性(参照表1）
- 采用H1或U6启动子保证在大多数哺乳动物细胞中高水平表达
高通量兼容
- 快速简便的实验设计
- 可同时检测多个样
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HEK293细胞中共转染miR-125a 抑制剂表达质粒 (GeneCopoeia HmiR-AN0094-AM01) 、miR-125a 前体表达质粒(GeneCopoeia HmiR0309-MR03) 和miR-125a的靶标克隆质粒(GeneCopoeia HmiT019205-MT02：将LIN28 的3’UTR克隆到Gaussia荧光素酶-碱性磷酸酶双报告载体)。转染24小时后检测报告基因Gaussia 荧光素酶和参照基因碱性磷酸酶的活性。只转染Gluc-LIN28-3’-UTR质粒的细胞中Gaussia 荧光素酶与碱性磷酸酶活性比值设为1 (左数第一条柱)，用来标准化其他细胞样品中的报告基因活性。结果表明miR-125a抑制了Gluc-LIN28-3’-UTR中Gluc 70%以上的活性（左数第二条柱）。这种抑制效应可被导入的不同剂量的miR-125a抑制剂表达质粒特异性的阻遏。转染100 ng 抑制剂表达质粒时，细胞内报告基因/参照基因活性比值（右数第一条）已超出单独转染Gluc-LIN28-3’-UTR报告质粒的对照组。这说明miR-125a抑制剂可以阻遏外源和内源性的miR-125a对靶标基因的抑制，提高GLuc-LIN28-3’-UTR转录本的翻译水平。
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图1. 不同剂量miArrest™ miRNA抑制剂克隆对靶miRNA抑制作用的对比 |
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| 表1. 基于克隆表达载体的和化学合成的miRNA抑制剂的比较 | |||||||||||||||||||||||||||||
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miRNA 抑制剂克隆导入细胞后表达的miRNA抑制剂(miArrest)特异地结合它们的靶miRNA。miRNA抑制剂转录后折叠形成一个稳定的陷阱结构，可以结合两分子靶miRNA。 miArrest™高效、特异地结合细胞内成熟的靶miRNA，阻止了miRNA对靶标mRNA的降解或者翻译抑制，上调miRNA靶标基因的表达。 独特的载体设计确保最大的特异性、抑制强度、抑制作用持久度和最低的脱靶效应。 |
图2. miRNA抑制剂表达克隆的作用机理 |
载体
miRNA抑制剂表达克隆载体
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慢病毒载体的miRNA抑制剂表达克隆 |
GeneCopoeia提供客户定制的慢病毒载体miRNA抑制剂表达克隆，周期2-5周。
化学合成的miRNA抑制剂
需要优化设计的miRNA抑制剂，点击了解更多
克隆套装
miRNA抑制剂表达克隆套装
GeneCopoeia除了提供单个克隆，也提供预制和定制的miRNA抑制剂表达克隆套装。了解详情请点击联系我们或拨打400-6020-200。
| 货号 | 产品描述 | miRNA | 载体 | 价格 |
| MIC-HG-AM03 | Human whole genome miRNA inhibitor clone set (lentiviral) | 994 | pEZX-AM03 | Inquire |
| Various | Human cancer miRNA inhibitor clone sets (lentiviral) | Various View all cancer sets below |
pEZX-AM03 | Inquire |
| MIC-MG-AM03 | Mouse genome miRNA inhibitor clone set (lentiviral) | 307 | pEZX-AM03 | Inquire |
| MIC-RG-AM03 | Rat genome miRNA inhibitor clone set (lentiviral) | 222 | pEZX-AM03 | Inquire |
人癌症miRNA抑制剂克隆套装(慢病毒载体)
| 货号 | 产品描述 | 癌症 | miRNA | 价格 |
| MIC-HCY-AM03 | Human bladder cancer miRNA inhibitor clone set | Bladder cancer | 63 | Inquire |
| MIC-HCN-AM03 | Human brain cancer miRNA inhibitor clone set | Brain cancer | 206 | Inquire |
| MIC-HCB-AM03 | Human breast cancer miRNA inhibitor clone set | Breast cancer | 158 | Inquire |
| MIC-HCV-AM03 | Human cervical cancer miRNA inhibitor clone set | Cervical cancer | 26 | Inquire |
| MIC-HCI-AM03 | Human cholangiocarcinoma miRNA inhibitor clone set | Cholangiocarcinoma | 25 | Inquire |
| MIC-HCC-AM03 | Human colorectal cancer miRNA inhibitor clone set | Colorectal cancer | 175 | Inquire |
| MIC-HCW-AM03 | Human endometrial cancer miRNA inhibitor clone set | Endometrial cancer | 86 | Inquire |
| MIC-HCG-AM03 | Human gastric cancer miRNA inhibitor clone set | Gastric cancer | 76 | Inquire |
| MIC-HCT-AM03 | Human head and neck cancer miRNA inhibitor clone set | Head and Neck cancer | 188 | Inquire |
| MIC-HCH-AM03 | Human hepatocellular carcinoma miRNA inhibitor clone set | Hepatocellular Carcinoma | 170 | Inquire |
| MIC-HCK-AM03 | Human kidney cancer miRNA inhibitor clone set | Kidney cancer | 67 | Inquire |
| MIC-HCX-AM03 | Human leukemia miRNA inhibitor clone set | Leukemia | 163 | Inquire |
| MIC-HCL-AM03 | Human lung cancer miRNA inhibitor clone set | Lung cancer | 178 | Inquire |
| MIC-HCZ-AM03 | Human lymphoma miRNA inhibitor clone set | Lymphoma | 112 | Inquire |
| MIC-HCM-AM03 | Human melanoma miRNA inhibitor clone set | Melanoma | 131 | Inquire |
| MIC-HCE-AM03 | Human mesothelioma miRNA inhibitor clone set | Mesothelioma | 21 | Inquire |
| MIC-HCR-AM03 | Human myeloma miRNA inhibitor clone set | Myeloma | 45 | Inquire |
| MIC-HCO-AM03 | Human ovarian cancer miRNA inhibitor clone set | Ovarian cancer | 174 | Inquire |
| MIC-HCP-AM03 | Human pancreatic cancer miRNA inhibitor clone set | Pancreatic cancer | 166 | Inquire |
| MIC-HCA-AM03 | Human pituitary adenoma miRNA inhibitor clone set | Pituitary Adenoma | 33 | Inquire |
| MIC-HCQ-AM03 | Human prostate cancer miRNA inhibitor clone set | Prostate cancer | 153 | Inquire |
| MIC-HCS-AM03 | Human sarcoma miRNA inhibitor clone set | Sarcoma | 27 | Inquire |
| MIC-HCJ-AM03 | Human thyroid carcinoma miRNA inhibitor clone set | Thyroid Carcinoma | 55 | Inquire |
| MIC-HCU-AM03 | Human uterine leiomyoma miRNA inhibitor clone set | Uterine Leiomyoma | 40 | Inquire |
相关产品
发表的文章
- Nguyen, T. et al (2013) MiR-24 Promotes the Survival of Hematopoietic Cells. PloS one, 8(1): e55406. doi:10.1371/journal.pone.0055406. [miR-24 miRNA inhibitor].
- Sharma, S. et al. (2012) Repression of miR-142 by p300 and MAPK is required for survival signalling via gp130 during adaptive hypertrophy. EMBO Molecular Medicine, Volume 4, Issue 7, pages 617–632, July 2012. [miR142-5p inhibitor].
- Kong, Y. et al. (2012) The Deoxycholic Acid Targets miRNA-dependent CAC1 Gene Expression in Multidrug Resistance of Human Colorectal Cancer. The International Journal of Biochemistry & Cell Biology, Available online 10 August 2012. [miR-199a-5p].
- Samakoglu, S. et al. (2012) Preclinical Rationale for Combining an EGFR Antibody with Cisplatin/Gemcitabine for the Treatment of NSCLC. Cancer Genomics and Proteomics March-April 2012 vol. 9 no. 2 77-92. [hsa-miRNA-29b synthetic inhibitor].
- Izumiya, M. et al. (2011) Systematic exploration of cancer-associated microRNA through functional screening assays. Cancer Science Volume 102, Issue 9. [miRNA inhibitor and precursor miRNA].
