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核酸荧光染料

DNA凝胶染料

GeneCopoeia公司最新推出了一系列核酸荧光染料产品作为EB替代品,具有安全、灵敏度高以及产品性能稳定等优点。

GreenView™, GreenView™ Plus, GreenView™ Ultra, and RedView™是GeneCopoeia公司最新研发具有安全无毒,灵敏度高,稳定性好的EB替代品, 使用方法同EB,可用于前染法和后染法,条带整齐不弯曲。GreenView™, GreenView™ Plus, and GreenView™ Ultra可在紫外灯或蓝光仪下观察发绿色荧光;RedView™可在紫外灯下观察发红色荧光。

核酸电泳是核酸研究的重要手段之一,其中溴化乙啶(EB)因其低廉的价格是分子生物学中使用最早和最为广泛的核酸电泳染色试剂。但是EB是一种强诱变剂,具有高致癌性,其废弃液必须进行净化处理,而且EB的检测灵敏度有限以及背景信号较高,目前正逐渐被灵敏度高以及安全可靠的新型核酸荧光染料所取代。

产品特点

  • 安全无毒,无致突变
  • 稳定性好
  • 灵敏度高,条带整齐

 

Green_RedView Figure 1

Figure 1. Gel stain results with GreenView™, GreenView™ Plus, and GreenView™ Ultra (Left), and comparison results with SYBR Safe™ and GelGreen™ (Right). Two-fold serial dilutions of a 1 kb DNA ladder (NEB, Cat # N0468S) were loaded onto each gel in 3 lanes in the amounts of 25 ng, 50 ng and 100 ng (left to right), then post-stained with GreenView™, GreenView™ Plus, GreenView™ Ultra, SYBR Safe™ and GelGreen™, respectively. Results show GreenView™ has similar sensitivity with SYBR Safe™ and GelGreen™, GreenView™ Plus and GreenView™ Ultra have much better sensitivity.

Green_RedView Figure 1

Figure 2. Gel stain results with RedView™, and GelRed™. Two-fold serial dilutions of a 1 kb DNA ladder (NEB, Cat # N0468S) were loaded onto each gel in 3 lanes in the amounts of 25 ng, 50 ng and 100 ng (left to right), then post-stained with RedView™, and GelRed™, respectively. Results show RedView™ has similar sensitivity with GelRed™.

此外,GreenView™, GreenView™ Plus, and RedView™ 具有较低的细胞膜通透性,不易与细胞内DNA结合,安全性优于SYBR Safe。

Green_RedView Figure 2

Figure 3. Cell permeability tests with SYBR Safe, GreenView, GreenView Plus, and RedView. Hela cells were incubated at 37℃ with 1X SYBR Safe, GreenView, GreenView Plus, and RedView for 30 min. GreenView Plus and RedView could not penetrate cell membranes to bind DNA in living cells, and were much safer than SYBR Safe, which rapidly entered cells and stained nuclei.

DNA上样染料

SafeGreen™和SafeRed™是GeneCopoeia最新推出的安全灵敏,操作简单的次世代核酸电泳染料,电泳前只需将染料与DNA混合,上样,即可获得条带清晰的染胶效果,整个过程无需前染和后染。

产品特点

  • 即用型,操作简单
  • 安全无毒
  • 灵敏度高
  • 成本低

SafeGreen™ 和 SafeRed™ Loading Dye 含 6 × DNA 上样缓冲液中及两类常用的示踪染料 (Xylene Cyanol FF 和 Bromophenol Blue),用以监测电泳期间琼脂糖凝胶条带(呈现紫色和蓝色两个条带)迁移情况。DNA条带可通过 LED 或紫外光直接观察,无需前染和后染步骤。

Green_RedView Figure 4

Figure 4. Gel staining results with SafeGreen™ and SafeRed™ premixed with DNA ladder. 1 kb DNA Ladder (Cat# D001) was mixed with SafeGreen™ or SafeRed™ at a 5:1 ratio, then loaded onto each well. Gels were directly imaged on a UV transilluminator.

SafeGreen™ 和 SafeRed™ 无法穿透细胞膜进入活细胞,从而降低了细胞毒性(Figure 5)。

Green_RedView Figure 5

Figure 5. Cell permeability tests with SYBR Safe™, SafeGreen™, and SafeRed™. Hela cells were incubated at 37℃ with 1X SYBR Safe™, SafeGreen™, and SafeRed™ for 30 min. SafeGreen™ and SafeRed™ could not penetrate cell membranes to bind DNA in living cells, and were much safer than SYBR Safe™, which rapidly entered cells and stained nuclei.

订购

核酸荧光染料选购指南

  GreenView GreenView Plus GreenView Ultra RedView
灵敏度 (dsDNA) Sensitive
(~2 ng)
Highly sensitive
(~0.2 ng)
Ultra sensitive
(~60 pg)
Sensitive
(~1 ng)
安全性
Gel electrophoresis
dsDNA
ssDNA    
RNA    
前染法  
后染法      
胶回收  
激发光源 蓝光/紫外 蓝光/紫外 蓝光/紫外 紫外
滤光片 SYBR filter SYBR filter SYBR filter EB filter

 

核酸电泳荧光染料

购买 货号 产品名称 描述 规格 价格(¥)
N100 GreenView DNA Gel Stain 无毒、无突变的DNA凝胶染料 500 µl  400 
N101 GreenView Plus DNA Gel Stain 无毒、无突变的DNA凝胶染料 500 µl  600 
N103 GreenView Ultra DNA Gel Stain 无毒、无突变的DNA凝胶染料 500 µl  800 
N102 RedView DNA Gel Stain 无毒、无突变的DNA凝胶染料 500 µl  600 
D012 SafeGreen Loading Dye 无毒、无突变的DNA凝胶染料 1 mL 300
D013 SafeRed Loading Dye 无毒、无突变的DNA凝胶染料 1 mL 300
D001 1kb DNA Ladder DNA ladder 250 µl 200
D002 1kb Plus DNA Ladder DNA ladder 250 µl 270
D003 100bp Plus DNA Ladder DNA ladder 250 µl 270
D016 DNA Loading Buffer (6X) DNA 上样缓冲液 5 ml 70