产品简介
GLuc-ON™ 启动子报告克隆在GLuc报告基因上游插入一段1.2~1.5 kb的序列，这段插入序列与基因转录起始位点（TSS）上游大约1.5kb到下游200bp之间的5'侧翼序列一致，可以通过观察荧光素酶的活性来研究启动子对基因的调节作用。
GeneCopoeia可提供预制和定制的GLuc-ON™ 启动子克隆，包括单报告基因载体系统和双报告基因载体系统。单报告基因载体系统以Gluc、mCherry或GFP作为启动子的报告基因；双报告基因载体系统以Gluc作为启动子报告基因，以分泌型碱性磷酸酶 （SEAP）作为信号标准化的内参，可在活细胞中对超过20,000种人和18,000种小鼠启动子进行实时活性分析。
图1. GLuc-ON™ 启动子报告克隆的双报告基因检测原理图。
优势 |
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活细胞分析
- 分泌型Gluc报告子
- 无需裂解细胞
- 节省样本，减少突变，简化试验流程（如脉冲追踪分析等）
实时研究
- 可获得即时检测数据
- 与实时活性分析过程类似
分泌型双报告系统
- 分泌型Gluc和分泌型碱性磷酸酶
- 对多样本常规转染进行精确比较
高通量兼容
- 可用于信号通路研究
- 可进行高通量研究
高灵敏度
- GLuc比萤火虫或Renilla荧光素酶灵敏度高1000倍
使用方便
- 所有启动子报告克隆均可直接用于细胞转染
Gaussia 荧光素酶
GLuc-ON™ 启动子克隆采用经过修饰的GLuc（mGLuc）作为报告基因，能产生强烈且稳定的荧光信号，克服了人野生型GLuc（wtGLuc）信号衰退快的缺点。
图2. mGLuc (蓝色) 和 wtGLuc (红色)的信号稳定性比较。左侧：含有稳定剂的缓冲液；右侧：常规缓冲液。
双报告系统
GLuc-ON™ 启动子克隆可以将分泌型碱性磷酸酶（SEAP）作为第二个报告子和内参。这种双报告系统可以对多样本常规转染进行精确比较。
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图3. 不同启动子在H1B1B和HEK293T细胞中的活性分析。双报告系统启动子克隆和对照以两个重复转染进两种细胞中。转染24小时（(HEK293T）和48小时（H1B1B）后分析样品。NEG（包含非启动子序列）和EMPTY（载体不含启动子）作为阴性对照。 |
载体和对照
载体类型 |
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| 载体 | 报告基因 | 内参基因 | 筛选标记 | 载体类型 |
| pEZX-PG04 | Gaussia luciferase (GLuc) | 分泌型碱性磷酸酶(SEAP) | Puromycin | 非病毒 |
| pEZX-PG02 | Gaussia luciferase (GLuc) | N/A* | Puromycin | 非病毒 |
| pEZX-PF02 | eGFP | N/A* | Puromycin | 非病毒 |
| pEZX-PM02 | mCherry | N/A* | Puromycin | 非病毒 |
| pEZX-LvPG04 | Gaussia luciferase (GLuc) | 分泌型碱性磷酸酶((SEAP) | Puromycin | 慢病毒 |
| pEZX-LvPG02 | Gaussia luciferase (GLuc) | N/A* | Puromycin | 慢病毒 |
| pEZX-LvPF02 | eGFP | N/A* | Puromycin | 慢病毒 |
| pEZX-LvPM02 | mCherry | N/A* | Puromycin | 慢病毒 |
| pEZX-LvPM03 | tdTomato | N/A* | Puromycin | 慢病毒 |
* SEAP可以在分泌型载体中表达。
对照克隆和SEAP表达克隆
Tear Away License
订购
预制的启动子报告克隆GeneCopoeia提供超过20,000种人和18,000种小鼠不同载体的预制启动子报告克隆。
慢病毒载体的启动子报告克隆GeneCopoeia提供客户定制的慢病毒载体启动子报告克隆。 了解慢病毒载体价格信息，请查询。
定制启动子报告克隆 |
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需要定制克隆来比较启动子不同长度或不同区域的功能，请发邮件到 support@igenebio.com 或拨打4006-020-200
| 下载 |
| GLuc-ON™ Promoter Reporter Clones Cancellation, QA, License, and Warranty |
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发表的文章
使用GLuc-ON™ 启动子报告克隆发表的文章
< 2018
- Smith, S.R., et al. (2018). The phosphatase PPM1A controls monocyte-to-macrophage differentiation. Scientific Reports. DOI: 10.1038/s41598-017-18832-7 [GLuc-ON™ PPM1A lentiviral promoter-reporter clone]
- Bueno, M., et al. (2018). ATF3 represses PINK1 gene transcription in lung epithelial cells to control mitochondrial homeostasis. Aging Cell. DOI: 10.1111/acel.12720 [GLuc-ON™ PINK1 promoter-reporter clone]
- Wang, J., et al. (2018). Targeting dual specificity protein kinase TTK attenuates tumorigenesis of glioblastoma. Oncotarget. DOI: 10.18632/oncotarget.23152 [GLuc-ON™ TTK promoter-reporter clone]
- Mu, N., et al. (2018). PRL- 3 is a potential glioblastoma prognostic marker and promotes glioblastoma progression by enhancing MMP7 through the ERK and JNK pathways. Theranostics. DOI: 10.7150/ thno .22699 [GLuc-ON™ MMP7 promoter-reporter clone]
- Li, C-W., et al. (2018). Eradication of Triple-Negative Breast Cancer Cells by Targeting Glycosylated PD-L1. Cancer Cell. DOI: 10.1016/j.ccell.2018.01.009 [GLuc-ON™ B3GNT3 promoter-reporter clone]
- Tripathi, R., et al. (2018). Abl and Arg mediate cysteine cathepsin secretion to facilitate melanoma invasion and metastasis. Science Signaling. DOI: 10.1126/scisignal.aao0422 [GLuc-ON™ Human cathepsin L, cathepsin B, Sp1, and Ets1 promoter-reporter clones]
- Armelloni, S., et al. (2018). NeuroD Expression in Podocytes and Interrelationships with Nephrin at Both Nuclear and Cytoplasmic Sites. Cellular Physiology and Biochemistry. DOI: 10.1159/000488818 [GLuc-ON™ Nephrin, Kirrel2 promoter-reporter clones]
- Liu, D., et al. (2018). Squalene epoxidase drives NAFLD-induced hepatocellular carcinoma and is a pharmaceutical target. Science Translational Medicine. DOI: 10.1126/scitranslmed.aap9840 [GLuc-ON™ SQLE promoter-reporter clone]
- Yang, T., et al. (2018). Brain ischemic preconditioning protects against ischemic injury and preserves the blood-brain barrier via oxidative signaling and Nrf2 activation. Redox Biology. DOI: 10.1016/j.redox.2018.05.001 [GLuc-ON™ Nrf2 lentiviral promoter-reporter clone]
- Chen, Y-C., et al. (2018). ATOH1/RFX1/RFX3 transcription factors facilitate the differentiation and characterisation of inner ear hair cell-like cells from patient-specific induced pluripotent stem cells harbouring A8344G mutation of mitochondrial DNA. Cell Death & Disease. DOI: 10.1038/s41419-018-0488-y2 [GLuc-ON™ MYO7A lentiviral promoter-reporter clone]
- Taha, A.S., et al. (2018). Tu1958 – Aspirin, NSAIDs, and Dysplastic Colonic Polyps – Lessons from Bowel Cancer Screening. Gastroenterology. DOI: 10.1016/S0016-5085(18)33561-3 [GLuc-ON™ COX-2 promoter-reporter clone]
- Jia, Y., et al. (2018). The novel KLF4/PLAC8 signaling pathway regulates lung cancer growth. Cell Death & Disease. DOI: 10.1038/s41419-018-0580-3 [GLuc-ON™ PLAC8 wild type and mutant promoter-reporter clones]
- Collmann, F.M., et al. (2018). Imaging Reporter Strategy to Monitor Gene Activation of Microglia Polarisation States under Stimulation. Journal of Neuroimmune Pharmacology. DOI: 10.1007/s11481-018-9789-2 [GLuc-ON™ Ym1, Fcgr3 promoter-reporter clones]
- Biddlestone, J., et al. (2018). SINHCAF/FAM60A and SIN3A specifically repress HIF 2α expression. Biochemical Journal. DOI: 10.1042/BCJ20170945 [GLuc-ON™ HIF 2α promoter-reporter clone]
- Xiang, S., et al. (2018). Crosstalk of NF-κB/P65 and LncRNA HOTAIR-Mediated Repression of MUC1 Expression Contribute to Synergistic Inhibition of Castration-Resistant Prostate Cancer by Polyphyllin 1-Enzalutamide Combination Treatment. Cellular Physiology and Biochemistry. DOI: 10.1159/000490028 [GLuc-ON™ HOTAIR, MUC1 promoter-reporter clones]
- Hildebrandt, S., et al,. (2018). Glucocorticoids suppress Wnt16 expression in osteoblasts in vitro and in vivo. Scientific Reports. DOI: 10.1038/s41598-018-26300-z [GLuc-ON™ Wnt16 promoter-reporter clone]
- Ye, D-J., et al. (2018). Discovery of ezrin expression as a potential biomarker for chemically induced ocular irritation using human corneal epithelium cell line and a reconstructed human cornea-like epithelium model. Toxicological Sciences. DOI: 10.1093/toxsci/kfy134 [GLuc-ON™ EZR promoter-reporter clone]
- Lu, Y., et al. (2018). Th9 Cells Represent a Unique Subset of CD4+ T Cells Endowed with the Ability to Eradicate Advanced Tumors. Cancer Cell. DOI: 10.1016/j.ccell.2018.05.004 [GLuc-ON™ Traf6 promoter-reporter clone]
- Al-Hattab, D.S., et al. (2018). Scleraxis regulates Twist1 and Snai1 expression in the epithelial-to-mesenchymal transition. American Journal of Physiology-Heart and Circulatory Physiology. DOI: 10.1152/ajpheart.00092.2018 [GLuc-ON™ Twist promoter-reporter clone]
- Subramani, R., et al. (2018). FOXC1 plays a crucial role in the growth of pancreatic cancer. Oncogenesis. DOI: 10.1038/s41389-018-0061-7 [GLuc-ON™ FOXC1 promoter-reporter clone]
< 2017
- Druzd, D., et al. (2017). Lymphocyte Circadian Clocks Control Lymph Node Trafficking and Adaptive Immune Responses. Immunity. DOI: 10.1016/j.immuni.2016.12.011 [GLuc-ON™ S1pr1 promoter-reporter clone]
- Alvarado, A.G., et al. (2017). Glioblastoma Cancer Stem Cells Evade Innate Immune Suppression of Self-Renewal through Reduced TLR4 Expression. Cell Stem Cell. DOI: 10.1016/j.stem.2016.12.001 [GLuc-ON™ SOX2, OCT4, and NANOG promoter-reporter clones]
- Tang, Q., et al. (2017). Emodin Increases Expression of Insulin-Like Growth Factor Binding Protein 1 through Activation of MEK/ERK/AMPKα and Interaction of PPARγ and Sp1 in Lung Cancer. Cellular Physiology and Biochemistry. DOI: 10.1159/000456281. [GLuc-ON™ IGFBP1 promoter-reporter clone]
- Wang, X., et al. (2017). Macrophages induce AKT/β-catenin-dependent Lgr5+ stem cell activation and hair follicle regeneration through TNF. Nature Communications. DOI: 10.1038/ncomms14091 [GLuc-ON™ LGR5 promoter-reporter clone]
- Feng, W., et al. (2017). SOX10 induced Nestin expression regulates cancer stem cell properties of TNBC cells. Biochemical and Biophysical Research Communications. DOI: 10.1016/j.bbrc.2017.02.014 [NES promoter-reporter clone]
- Wu, D.W., et al. (2017). The YAP1/SIX2 axis is required for DDX3 -mediated tumor aggressiveness and cetuximab resistance in KRAS -wild -type colorectal cancer. Theranostics. DOI: 10.7150/ thno .18175 [GLuc-ON™ YAP1 and SIX2 promoter-reporter clones]
- Lim, J.P., et al. (2017). YBX1 gene silencing inhibits migratory and invasive potential via CORO1C in breast cancer in vitro. BMC Cancer. DOI: 10.1186/s12885-017-3187-7 [GLuc-ON™ CORO1C promoter-reporter clone]
- Cho, Y.S., et al. (2017). COMPOSITIONS COMPRISING A MITOFUSIN INHIBITOR FOR PROMOTING CELL REPROGRAMMING AND A USE THEREOF United States Patent Application 20170051254 [GLuc-ON™ Mfn1 promoter-reporter clone]
- Sun, J., et al. (2017). HMGA2 regulates CD44 expression to promote gastric cancer cell motility and sphere formation. Am J Cancer Res 2017;7(2) :260-274 [GLuc-ON™ c-Myc promoter-reporter clone]
- Mondello, P., et al. (2017). Panobinostat acts synergistically with ibrutinib in diffuse large B cell lymphoma cells with MyD88 L265 mutations. JC Insight. DOI: DOI.org/10.1172 [GLuc-ON™ MyD88 promoter-reporter clone]
- Shen, Z.H., et al. (2017). HOXA10 promotes nasopharyngeal carcinoma cell proliferation and invasion via inducing the expression of ZIC2. European Review for Medical and Pharmacological Sciences 2017; 21: 945-952 [GLuc-ON™ ZIC2 promoter-reporter clone]
- Shen, C.J., et al. (2017). MMP1 expression is activated by Slug and enhances multi-drug resistance (MDR) in breast cancer. PLoS ONE. DOI: 10.1371/journal.pone.0174487 [GLuc-ON™ MMP1 promoter-reporter clone]
- Wu, J.J., et al. (2017). Interplay of DNA methyltransferase 1 and EZH2 through inactivation of Stat3 contributes to β-elemene-inhibited growth of nasopharyngeal carcinoma cells. Scientific eports. DOI: 10.1038/s41598-017-00626-6 [GLuc-ON™ EZH2 promoter-reporter clone]
- Qu, L.P., et al. (2017). CDH17 is a downstream effector of HOXA13 in modulating the Wnt/ β -catenin signaling pathway in gastric cancer. European Review for Medical and Pharmacological Sciences 2017; 21: 1234-1241 [GLuc-ON™ CDH17 promoter-reporter clone]
- Morel, A. P., et al. (2017). A stemness-related ZEB1–MSRB3 axis governs cellular pliancy and breast cancer genome stability. Nature Medicine. DOI: 10.1038/nm.4323 [GLuc-ON™ MSRB3 promoter-reporter clone]
- Liu, J., et al. (2017). DDA1 is induced by NR2F6 in ovarian cancer and predicts poor survival outcome. European Review for Medical and Pharmacological Sciences 2017; 21: 1206 -1213 [GLuc-ON™ DDA1 promoter-reporter clone]
- Wang, L., et al. (2017). FSCN1 is upregulated by SNAI2 and promotes epithelial to mesenchymal transition in head and necksquamous cell carcinoma. Cell Biology International. DOI: 10.1002/cbin.10786 [GLuc-ON™ FSCN1 promoter-reporter clone]
- Larson, M.E., et al. (2017). Selective lowering of synapsins induced by oligomeric α-synuclein exacerbates memory deficits. Proc. Natl. Acad. Sci. U.S.A.. DOI: 10.1073/pnas.1704698114 [GLuc-ON™ SYN2 promoter-reporter clone]
- Azeem, W., et al. (2017). An androgen response element driven reporter assay for the detection of androgen receptor activity in prostate cells. PLoS ONE. DOI: 10.1371/journal.pone.0177861 [ARE promoter-reporter clone]
- Li, D., et al. (2017). Hepatic hypoxia-inducible factors inhibit PPARα expression to exacerbate acetaminophen induced oxidative stress and hepatotoxicity. Free Radical Biology and Medicine. DOI: 10.1016/j.freeradbiomed.2017.06.002 [GLuc-ON™ PPARα promoter-reporter clone]
- TianJ., et al. (2017). E2F8 confers cisplatn resistance to ER+ breast cancer cells via transcriptionally activating MASTL. Biomedicine & Pharmacotherapy. DOI: 10.1016/j.biopha.2017.05.118 [MASTL promoter-reporter clone]
- Xie, Q. nd Cao, X. (2017). Mitochondrial transcription factor A protects human retinal endothelial cell injury induced by hypoxia. Tropical Journal of Pharmaceutic al Research . DOI: 10.4314/tjpr.v16i6.8 [NF-κB Promoter-Reporter clone]
- Yang, B., et al. (2017). Yes‐associated protein 1 promotes the differentiation and mineralization of cementoblast. Journal of Cellular Physiology. DOI: 10.1002/jcp.26089 [DSPP Promoter-Reporter clone]
- Erdmann, K., et al. (2017). Induction of alpha-methylacyl-CoA racemase by miR-138 via up-regulation of β-catenin in prostate cancer cells. Journal of Cancer Research and Clinical Oncology. DOI:10.1038/nrg.2016.134 [GLuc-ON™ CTNNB1 Promoter-Reporter clone]
- Thiagarajan, P.S., et al. (2017). STAT3 activation by leptin receptor is essential for TNBC stem cell maintenance. Endocrine-Related Cancer. DOI: 10.1530/ERC-16-0349 [GLuc-ON™ NANOG, SOX2, and OCT4 Promoter-Reporter clones]
- Madar-Shapiro, L., et al. (2017). Predicting the Risk to Develop Preeclampsia in the First Trimester Combining Promoter Variant -98A/C of LGALS13 (Placental Protein 13), Black Ethnicity, Previous Preeclampsia, Obesity, and Maternal Age. Fetal Diagnosis and Therapy. DOI: 10.1159/000477933 [GLuc-ON™ LGALS13 Promoter-Reporter clone]
- Huang, L.S., et al. (2017). Lysocardiolipin acyltransferase regulates TGF-β mediated lung fibroblast differentiation. Free Radical Biology and Medicine. DOI: 10.1016/j.freeradbiomed.2017.07.023 [GLuc-ON™ LYCAT Promoter-Reporter clone]
- McCutcheon, S., et al. (2017). A multiscale fluidic device for the study of dendrite-mediated cell to cell communication. Biomedical Microdevices. DOI: 10.1007/s10544-017-0212-1 [GLuc-ON™ mouse Tnfsf11 lentiviral promoter-reporter clone]
- Correa, D., et al. (2017). Non-destructive/non-invasive imaging evaluation of cellular differentiation progression during in vitro MSC-derived chondrogenesis. Tissue Engineering. DOI: 10.1089/ten.TEA.2017.0125 [GLuc-ON™ Sox9 and Aggrecan lentiviral promoter-reporter clones]
- Jiang, Z., et al. (2017). Ribosome profiling reveals translational regulation of mammalian cells in response to hypoxic stress. BMC Genomics. DOI: 10.1186/s12864-017-3996-8 [GLuc-ON™ uORFpromoter-reporter clone]
- Zhang, Q., et al. (2017). Repression of ESR1 transcription by MYOD potentiates letrozle-resistance in ERα-positive breast cancer cells. Biochemical and Biophysical Research Communications. DOI: 10.1016/j.bbrc.2017.08.082 [GLuc-ON™ ESR1 promoter-reporter clone]
- Zheng, F., et al. (2017). The enhancement of combination of berberne and metformn in inhibition of DNMT1 gene expression through interplay of SP1 and PDPK1. Journal of Cellular and Mlecular Medicine. DOI: 10.1111/jcmm.13347 [GLuc-ON™ DNMT1 promoter-reporter clone]
- Zhu, M. (2017). Hyperlipidemia-induced MicroRNA- 155-5p improves β -cell Function by targeting Mafb. Ph.D. Thesis, Universität München [GLuc-ON™ mouse IL6 promoter-reporter clone]
- Yao, J., et al. (2017). Vascular endothelium plays a key role in directing pulmonary epithelial cell differentiation. Journal of Cell Biology. DOI: 10.1083/jcb.201612122 [GLuc-ON™ albumin promoter-reporter clone]
- Yang, W.W., et al. (2017). Epiregulin Promotes Lung Metastasis of Salivary Adenoid Cystic Carcinoma. Theranostics. DOI: 10.7150/ thno.19712 [GLuc-ON™ CDH1 promoter-reporter clone]
- Yoshimatsu, G., et al. (2017). Pancreatic Beta Cell-derived IP-10/CXCL10 Isletokine Mediates Early Loss of Graft Function in Islet Cell Transplantation. Diabetes. DOI: 10.2337/db17-0578 [GLuc-ON™ mouse IP-10 promoter-reporter clone]
- Zhou, Y.F., et al. (2017). Tumor necrosis factor-α and interleukin-6 suppress microRNA-1275 transcription in human adipocytes through nuclear factor-κB. Molecular Medicine Reports. DOI: 10.3892/mmr.2017.7392 [GLuc-ON™ miR-1275 promoter-reporter clone]
- Chen, G., et al. (2017). GENERATING GABAergic NEURONS IN BRAINS. United States Patent Application 20170239373 [GLuc-ON™ LMTK2 promoter-reporter clone]
- Dey, I. and Bradbury, N.A. (2017). Activation of TPA-response element present in human Lemur Tyrosine Kinase 2 (lmtk2) gene increases its expression. Biochemistry and Biophysics Reports. DOI: 10.1016/j.bbrep.2017.09.006 [GLuc-ON™ hng2 promoter-reporter clone]
- Dey, I. and Bradbury, N.A. (2017). Activation of TPA-response element present in human Lemur Tyrosine Kinase 2 (lmtk2) gene increases its expression. Biochemistry and Biophysics Reports. DOI: 10.1016/j.bbrep.2017.09.006 [GLuc-ON™ LMTK2 promoter-reporter clone]
- Wang, J.X., et al. (2017). GLI2 induces PDGFRB expression and modulates cancer stem cell properties of gastric cancer. European Review for Medical and Pharmacological Sciences 2017; 21: 3857-3865 [GLuc-ON™ PDGFRB promoter-reporter clone]
- Zhang, H., et al. (2017). Inducible ATF3–NFAT axis aggravates podocyte injury. Journal of Molecular Medicine. DOI: 10.1007/s00109-017-1601-x [GLuc-ON™ NRATC1 promoter-reporter clone]
- Xiao, Q., et al. (2017). Activation of ERK and Mutual Regulation of Stat3 and SP1 Contribute to Inhibition of PDK1 Expression by Atractylenolide-1 in Human Lung Cancer Cells. Cellular Physiology and Biochemistry. DOI: 10.1159/000484387 [GLuc-ON™ promoter-reporter clone]
- Tang, Q., et al. (2017). Combination of Solamargine and Metformin Strengthens IGFBP1 Gene Expression Through Inactivation of Stat3 and Reciprocal Interaction Between FOXO3a and SP1. Cellular Physiology and Biochemistry. DOI: 10.1159/000484383 [GLuc-ON™ promoter-reporter clone]
- Hung, S., and Hsu, H. (2017). METHOD FOR INHIBITING SURVIVAL, TUMORIGENESIS AND METASTASIS OF CANCER CELLS AND/OR CANCER STEM CELLS. United States Patent Application 20170304386 [GLuc-ON™ Col17-A1 promoter-reporter clone]
- Singh, B.K., et al. (2017). Anti-melanogenic effect of Prunus davidiana extract in melan-a melanocyte through regulation of OCA-2, TRP-1 and tyrosinase. Korean Journal of Chemical Engineering. DOI: 10.1007/s11814-017-0241-8 [GLuc-ON™ promoter-reporter clone]
- Huang, L., et al. (2017). miR19b-3p promotes the growth and metastasis of colorectal cancer via directly targeting ITGB8. Am J Cancer Res 2017;7(10):1996-2008 [GLuc-ON™ FOXL2 wild type and mutant promoter-reporter clones]
- Xu, H.Y., et al. (2017). Metformn reduces SATB2-mediated osteosarcoma stem cell-like phenotype and tumor growth via inhibition of N-cadherin/NF- k B signaling. European Review for Medical and Pharmacological Sciences 2017; 21: 4516-4528 [GLuc-ON™ N-caderin promoter-reporter clone]
- Wilkinson, D.C., et al. (2017). Development of a Three‐Dimensional Bioengineering Technology to Generate Lung Tissue for Personalized Disease Modeling. Stem Cells Translational Medicine. DOI: 10.5966/sctm.2016-0192 [GLuc-ON™ ACTA2 promoter-reporter clone]
- Xiang, S.T., et al. (2017). HOTAIR-mediated reciprocal regulation of EZH2 and DNMT1 contribute to polyphyllin I-inhibited growth of castration-resistant prostate cancer cells in vitro and in vivo. Biochimica et Biophysica Acta (BBA) – General Subjects. DOI: 10.1016/j.bbagen.2017.12.001 [GLuc-ON™ promoter-reporter clones]
- Jiang, N., et al. (2017). YAP1 regulates prostate cancer stem cell-like characteristics to promote castration resistant growth. Oncotarget. DOI: 10.18632/oncotarget.23014 [GLuc-ON™ YAP1 promoter-reporter clone]
- Wang, N., et al. (2017). Identification of WT1 as determinant of heptatocellular carcinoma and its inhibition by Chinese herbal medicine Salvia chinensis Benth and its active ingredient protocatechualdehyde. Oncotarget. DOI: 10.18632/oncotarget.22406 [GLuc-ON™ human β-catenin promoter-reporter clone]
< 2016
- Liao, BM., et al. (2016) Flavin-Containing Monooxygenase 3 Reduces Endoplasmic Reticulum Stress in Lipid-Treated Hepatocytes. Molecular Endocrinology,DOI: http://dx.doi.org/10.1210/me.2015-1217. [FMO3 promoter clone and secreted pair luciferase kit]
- Dougherty, E.J., et al. (2016). Mineralocorticoid Receptor (MR) Trans-Activation of Inflammatory AP-1 Signaling: Dependence on DNA Sequence, MR Conformation, and AP-1 Family Member Expression. The Journal of Biological Chemistry. DOI: 10.1074/jbc.M116.732248 [human XIRP1 promoter clone]
- Li, L., et al. (2016). Inhibition of EZH2 via activation of SAPK/JNK and reduction of p65 and DNMT1 as a novel mechanism in inhibition of human lung cancer cells by polyphyllin I. Journal of Experimental & Clinical Cancer Research. DOI: 10.1186/s13046-016-0388-x [EZH2 promoter-reporter clone]
- Mao, C., et al. (2016). Double-edged-sword effect of IL-1β on the osteogenesis of periodontal ligament stem cells via crosstalk between the NF-κB, MAPK and BMP/Smad signaling pathways. Cell Death and Disease. DOI: 10.1038/cddis.2016.204 [BMP2 promoter-reporter clone]
- Gujar, R., et al. (2016). c-Src Suppresses Dendritic Cell Antitumor Activity via T Cell Ig and Mucin Protein-3 Receptor. The Journal of Immunology. DOI: 10.4049/jimmunol.1600104 [mouse wild tyoe and mutant HAVCR2 promoter-reporter clones]
- Chen, J., et al. (2016). Vitamin D Deficiency Promotes Liver Tumor Growth in Transforming Growth Factor-β/Smad3-Deficient Mice Through Wnt and Toll-like Receptor 7 Pathway Modulation. Scientific Reports. DOI: 10.1038/srep30217 [TLR7 Promoter-reporter clone]
- Nakajima, S., et al. (2016). Fungal-derived neoechinulin B as a novel antagonist of liver X receptor, identified by chemical genetics using hepatitis C virus cell culture system . Journal of Virology. DOI: 10.1128/JVI.00856-16 [SCD-1 promoter reporter clone]
- Zhu, L., et al. (2016). Knockdown of Sall4 inhibits intrahepatic cholangiocarcinoma cell migration and invasion in ICC-9810 cells. OncoTargets and Therapy. DOI: 10.2147/OTT.S107214 [PTEN and Bmi-1 promoter clones]
- Rehman, M., et al. (2016). PlexinD1 Is a Novel Transcriptional Target and Effector of Notch Signaling in Cancer Cells. PLoS ONE. DOI: 10.1371/journal.pone.0164660 [Hes1, PlexinD1promoter clones]
- Trueba-Saiz, A., et al. (2016). Circulating Insulin-like Growth Factor I Regulates Its Receptor in the Brain of Male Mice. Endocrinology. DOI: 10.1210/en.2016-1468 [IGF-IR promoter-Gaussia luciferase reporter lentiviral clone]
- Chu, Q., et al. (2016). Extracellular serglycin upregulates the CD44 receptor in an autocrine manner to maintain self-renewal in nasopharyngeal carcinoma cells by reciprocally activating the MAPK/β-catenin axis. Cell Death and Disease. DOI: 10.1038/cddis.2016.287 [GLuc-ON™ CD44 promoter-reporter clone]
- Xiang, S., et al. (2016). Activation of AMPKα mediates additive effects of solamargine and metformin on suppressing MUC1 expression in castration-resistant prostate cancer cells. Scientific Reports. DOI:10.1038/srep36721 [GLuc-ON™ MUC1 promoter-reporter clone]
- Holler, et al. (2016). Trehalose upregulates progranulin expression in human and mouse models of GRN haploinsufficiency: a novel therapeutic lead to treat frontotemporal dementia. Molecular Neurodegeneration. DOI: 10.1186/s13024-016-0114-3 [GLuc-ON™ PGRN promoter-reporter clone]
- Revandkar, A., et al. (2016). Inhibition of Notch pathway arrests PTEN-deficient advanced prostate cancer by triggering p27-driven cellular senescence. Nature Communications. DOI: 10.1038/ncomms13719 [GLuc-ON™ ADAM7 promoter clone]
- Xie, X., et al. (2016). c-Jun N-terminal kinase promotes stem cell phenotype in triple-negative breast cancer through upregulation of Notch1 via activation of c-Jun. Oncogene. DOI: 10.1038/onc.2016.417 [GLuc-ON™ Notch1 promoter-reporter clone]
< 2015
- Natunen, T., et al. (2015) Relationship between ubiquilin-1 and BACE1 in human Alzheimer's disease and APdE9 transgenic mouse brain and cell-based models. Neurobiology of disease,DOI: 10.1016/j.nbd.2015.11.005 [human BACE1 containing endogenous 5′UTR, BACE1-Myc (human BACE1 with a CMV promoter and a 3′-myc-tag sequence producing BACE1 protein containing a myc tag in the C-terminus)]
- C Authors, R Trivedi ., et al. (2015) JBC Papers in Press. Published on December 9, 2015 as Manuscript M115. 687152. JBC,http://www.jbc.org/cgi/doi/10.1074/jbc.M115.687152 [Runx2 promoter clones (Cat# MPRM13038)]
- Muralidharan, R., et al. (2015) HuR-targeted nanotherapy in combination with AMD3100 suppresses CXCR4 expression, cell growth, migration and invasion in lung cancer. Cancer Gene Therapy, ancer Gene Therapy (2015) 22, 581–590. DOI:10.1038/cgt.2015.55 [Promotor reporter clone for CXCR4]
- Maeng, YS., et al. (2015) TGFBIp regulates differentiation of EPC (CD133+ C‐kit+ Lin‐cells) to EC through activation of the Notch signaling pathway. STEM CELLS DOI: 10.1002/stem.2003. [pEZX-DLL1 pro- moter luciferase plasmids (-1.2 Kbp)]
- Li, C., et al. (2015) Noncanonical STAT3 Activation Regulates Excess TGF-β1 and Collagen I Expression in Muscle of Stricturing Crohn's Disease. The Journal of Immunology. DOI: 10.4049/jimmunol.1401779 [promoter reporter clone for human TGFB1 (HPRM13178-PG04), EndoFectin Lenti transfection reagent (EFP1003)]
- Zhang , Z., et al. (2015) BET Bromodomain Inhibition as a Therapeutic Strategy in Ovarian Cancer by Downregulating FoxM1. Theranostics. DOI:10.7150/thno.13178 [human FoxM1 promoter clone]
< 2014
- Ryu , JM., et al. (2014) Autotaxin‐LPA Axis regulates hMSC migration by adherent junction disruption and cytoskeletal rearrangement via LPAR1/3‐dependent PKC/GSK3β/β‐catenin and PKC/Rho GTPase Pathways . STEM CELLS. DOI: 10.1002/stem.1882 [CDH-1 promoter construct]
- Zhang, H., et al. (2014) The critical role of myeloid-derived suppressor cells and FXR activation in immune-mediated liver injury. Journal of Autoimmunity. DOI: 10.1016/j.jaut.2014.02.010 [MPRM11909-PG04]
- Rubie, C., et al. (2014) Chemokine receptor CCR6 expression is regulated by miR-518a-5p in colorectal cancer cells. Journal of translational medicine, 2014 Feb 21;12:48. DOI: 10.1186/1479-5876-12-48 [Luc CCR6 vector, Luc No CCR6 vector]
< 2013
- Klimosch, SN., et al. (2013) Functional TLR5 genetic variants affect human colorectal cancer survival. Cancer Research, 73(24); 7232–42 [Promoter Reporter ( MYD88 and ACAA1)]
- Shen, Q., et al. (2013) Adipocyte reporter assays: Application for identification of anti‐inflammatory and antioxidant properties of mangosteen xanthones. Molecular Nutrition & Food Research, DOI: 10.1002/mnfr.201300181 [Human Fabp4 promoter vector with eCFP reporter]
- Celardo, I., et al. (2013) Caspase-1 is a novel target of p63 in tumor suppression. Cell Death and Disease (2013) 4, e645;. DOI:10.1038/cddis.2013.175 [caspase-1 promoter plasmid in pEZX-PG02 vector]
- Zheng, H., et al. (2013) Glycogen synthase kinase-3 beta regulates Snail and β-catenin expression during Fas-induced epithelial–mesenchymal transition in gastrointestinal cancer. European Journal of Cancer, Available online 9 April 2013 [CDH1 promoter construct]
- Mills, L.D., et al. (2013) Loss of the Transcription Factor GLI1 Identifies a Signaling Network in the Tumor Microenvironment Mediating KRAS-Induced. Journal of Biological Chemistry, March 12, 2013,. DOI: 10.1074/jbc.M112.438846 [mouse SHH promoter clone]
- Schank, JR., et al (2013) Tacr1 Gene Variation and Neurokinin 1 Receptor Expression Is Associated with Antagonist Efficacy in Genetically Selected Alcohol-Preferring Rats. Biological Psychiatry Journal, PII: S0006-3223(13)00043-7. DOI:10.1016/j.biopsych.2012.12.027 [custom Tacr1 promoter luciferase construct]
< 2012
- Petrella, B., et al. (2012) Interleukin-1 beta and transforming growth factor-beta 3 cooperate to activate matrix metalloproteinase expression and invasiveness in A549 lung adenocarcinoma Cancer Letters, Volume 325, Issue 2, 28 December 2012, Pages 220–226 [MMP10 promoter clone]
