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蛋白染料及定量检测试剂盒

产品介绍

蛋白凝胶电泳及相关的Western-Blot是蛋白分析的主流技术 (Figure 1)。Applied BioProbes 公司提供两种蛋白染料: 1) Coomassie® Blue Fast Stain, 一种即用型的蛋白染料,无需甲醇或乙酸固定、或脱色; 2) eLuminol™ Protein Gel Stain,一种具有极高灵敏度的蛋白荧光染料,用于检测SDS-PAGE凝胶电泳中的总蛋白。Applied BioProbes 公司也提供一种专门检测磷酸化蛋白的荧光染料Phos-Tag™ Phosphoprotein Gel Stain。Applied BioProbes提供的 ECL Western Blotting Detection Kit用于WB检测HRP标记的抗体及其关联的抗原,具有快速检测、灵敏度极高等特点。另外,Applied BioProbes 也提供了两种蛋白定量分析试剂盒: 1) Bradford Protein Assay Kit;和 2) BCA Protein Assay Kit

Figure 1. Protein gel analysis workflow

蛋白染料选购指南

  Coomassie® Blue Fast Stain eLuminol™ Protein Gel Stain Phos-Tag™ Phosphoprotein Gel Stain
灵敏度 20 ng 0.5 ng 1 ng
操作性 Ready to use Supplied as stock solution Ready to use
耗时 2 hr (standard)
20 min (microwave)
90 min (standard)
30 min (microwave)
4 hr (standard)
2 hr (microwave)
检测方法 比色法 荧光 荧光
质谱兼容

 

Coomassie  

Figure 2. Gel stain results with Coomassie® Blue Fast Stain (left), eLuminol™ Protein Gel Stain (middle), and Phos-Tag™ Phosphoprotein Gel Stain, followed by eLuminol™ total protein stain (right). Two-fold serial dilutions (from left to right) of protein marker were separated via SDS-PAGE and then stained with above stains.

ECL化学发光检测试剂盒

                            

Figure 3. Detection of protein in Western Blots with ABP kit (left) and Millipore Kit (right).

                  Bradford Protein Assay Kit BCA Protein Assay Kit

Figure 4. BSA standard curve with Bradford Protein Assay Kit (left) and BCA Protein Assay Kit (right).

 

订购

Buy 货号 产品名称 描述 规格 价格(¥)
P001 Coomassie® Blue Fast Stain Colorimetric protein gel stain 500 mL 300

P003A
P003B
eLuminol™ Protein Gel Stain (1,000X) Fluorescent protein gel stain 500 µL
1 mL
600
1000
P005A Phos-Tag™ Phosphoprotein Gel Stain Phosphoprotein gel stain 500 mL 1000
P005B Phos-Tag™ Phosphoprotein Destain Solution Destain solution 500 mL 200
P007 ECL Western Blotting Detection Kit Western blotting reagent 100 mL 400
P010 Bradford Protein Assay Kit Protein quantitation 1000 assays 350
P011 BCA Protein Assay Kit Protein quantitation 1000 assays 500
P013 SDS-PAGE Sample Loading Buffer (5X) Loading buffer 5 mL 70