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二抗及链霉亲和素

产品介绍

 
免疫荧光技术是将免疫学方法(抗原抗体特异结合)与荧光标记技术结合起来研究特异蛋白抗原在细胞内分布的方法。由于荧光素所发的荧光可在荧光显微镜下检出,从而可对抗原进行细胞定位。
免疫荧光细胞化学是根据抗原抗体反应的原理,先将已知的抗原或抗体标记上荧光素制成荧光标记物,再用这种荧光抗体(或抗原)作为分子探针检查细胞或组织内的相应抗原(或抗体)。在细胞或组织中形成的抗原抗体复合物上含有荧光素,利用荧光显微镜观察标本,荧光素受激发光的照射而发出明亮的荧光,可以看见荧光所在的细胞或组织,从而对抗原或抗体进行定性、定位或定量检测。
 

免疫荧光检测方法根据待检测抗原的丰度采用不同的荧光标记策略。对于高丰度的抗原,可采用荧光标记一抗直接检测的方式;对于中等丰度的抗原,可采用荧光标记二抗的方式对待检测的抗原进行信号放大;对于低等丰度的抗原,可采用生物素标记抗体和荧光标记链霉亲和素的方式对待检测的抗原进行二级信号放大。在整个免疫荧光检测的过程中,荧光染料的性能(信号强度,稳定性,背景信号)对免疫荧光检测的结果发挥着关键性的作用。早期较常用的FITC荧光素标记抗体由于其信号弱,稳定性差,目前正逐渐被新型荧光染料(如Alexa Fluor, Dylight)所取代。

GeneCopoeia公司最新推出了一系列性能优良的Andy Fluor荧光染料。这些新型荧光染料跟传统的荧光染料(如FITC)相比,具有无可比拟的优越性。公司新推出了一系列Andy Fluor和生物素标记的二抗及相应的标记服务,为广大科研用户提供了广阔的选择。

产品特点

  • 荧光信号强;
  • 背景信号低;
  • 光稳定性好;
  • 多色荧光可供选择;
  • 仪器兼容性好;

 

荧光标记二抗及链霉亲和素选购指南

Secondary Antibody Streptavidin
Andy Fluor™ Dyes Other Fluorescent Labels Biotin & HRP Labels Andy Fluor™ & Cy® Dyes
Andy Fluor™ 350 Cy 3 Biotin Andy Fluor™ 350
Andy Fluor™ 405 Cy 5 HRP Andy Fluor™ 488
Andy Fluor™ 430 Cy 5.5   Andy Fluor™ 555
Andy Fluor™ 488 Cy 7   Andy Fluor™ 594
Andy Fluor™ 555 FITC   Andy Fluor™ 647
Andy Fluor™ 568     Cy®3
Andy Fluor™ 594     Cy®5
Andy Fluor™ 647      
Andy Fluor™ 680      
Andy Fluor™ 750      

Andy Fluor™ dye conjugates have brighter fluorescence than other fluorophores.

Figure 1. Comparison of relative fluorescence of goat anti-rabbit IgG antibody conjugates prepared from Andy Fluor™ 488, 555, and 647 with Cy®2, Cy®3, and Cy®5.

Figure 2. Flow cytometry comparison of the brightness of goat anti-mouse IgG antibody conjugates prepared from Andy Fluor™ 488, 555, and 647 with Cy®2, DyLight™ 488, Cy®3, DyLight™ 550, and DyLight™ 650.

Andy Fluor™ dye conjugates have better photostability than other fluorophores.


Figure 3. Comparison of the photobleaching rates of Andy Fluor™ 488 goat anti-mouse IgG (H+L) (L109B) with FITC goat anti-mouse IgG (H+L) (L146B). The cytoskeleton of HeLa cells was labeled with mouse monoclonal anti-α-tubulin antibody in combination with Andy Fluor™ 488 goat anti-mouse IgG (H+L) antibody (top series) or with mouse monoclonal anti-α-tubulin antibody in combination with FITC goat anti-mouse IgG (H+L) antibody (bottom series). The fluorescence imaging was taken at 60-second intervals (0, 60, and 120 seconds of exposure).

Figure 4. Immunofluorescent stain of α-tubulin in BEAS2BNNK cells. α-Tubulin in fixed and permeabilized BEAS2BNNK cells was labeled with anti-α-tubulin primary antibody, and then visualized with goat anti-mouse IgG antibodies conjugated with either Andy Fluor™ 488 (top, left), Andy Fluor™ 555 (top, right), Andy Fluor™ 568 (bottom, left), or Andy Fluor™ 647 (bottom, right). Nuclei are counterstained with DAPI (blue).

Figure 5. Immunofluorescent stain of CCSP in mouse lung tissue. FFPE samples of mouse lung were labeled with rabbit anti-CCSP primary antibody, and then visualized with green-fluorescent Andy Fluor™ 488 goat anti-rabbit IgG antibody (green). Nuclei were counterstained with DAPI (blue).

Figure 6. Immunofluorescent stain of CCSP in mouse lung tissue. FFPE samples of mouse lung were labeled with rabbit anti-CCSP primary antibody, and then visualized with either Andy Fluor™ 555 (red, left), or Andy Fluor™ 568 (red, right). Nuclei were counterstained with DAPI (blue).

Figure 7. Immunofluorescent stain of α-tubulin in BEAS2BNNK cells and CCSP in mouse lung tissue. α-Tubulin in fixed and permeabilized BEAS2BNNK cells was labeled with anti-α-tubulin primary antibody, followed by incubation with biotin goat anti-mouse IgG antibody, and then visualized with Andy Fluor™ 488 Streptavidin (green, left). FFPE samples of mouse lung were labeled with rabbit anti-CCSP primary antibody, followed by incubation with biotin goat anti-mouse IgG antibody, and then visualized with Andy Fluor™ 488 Streptavidin (green, middle and right). Nuclei were counterstained with DAPI (blue).

订购

Andy Fluor™ 标记二抗

春季促销,Andy Fluor™标记二抗全线产品6折优惠,促销期间:3月1日至5月31日。

购买 货号 产品名称 激发/发射波长 (nm) 规格 价格(¥) 促销价(¥)

L100A
L100B
Andy Fluor™ 350 Goat Anti-Mouse IgG (H+L) Antibody 345/440 100 µg
500 µg
300
600

180
360


L101A
L101B
Andy Fluor™ 350 Goat Anti-Rabbit IgG (H+L) Antibody 345/440 100 µg
500 µg
300
600
180
360

L102A
L102B
Andy Fluor™ 350 Goat Anti-Human IgG (H+L) Antibody 345/440 100 µg
500 µg
300
600
180
360

L103A
L103B
Andy Fluor™ 405M Goat Anti-Mouse IgG (H+L) Antibody 405/450 100 µg
500 µg
300
600
180
360

L104A
L104B
Andy Fluor™ 405M Goat Anti-Rabbit IgG (H+L) Antibody 405/450 100 µg
500 µg
300
600
180
360

L105A
L105B
Andy Fluor™ 405M Goat Anti-Human IgG (H+L) Antibody 405/450 100 µg
500 µg
300
600
180
360

L106A
L106B
Andy Fluor™ 430 Goat Anti-Mouse IgG (H+L) Antibody 430/540 100 µg
500 µg
300
600
180
360

L107A
L107B
Andy Fluor 430 Goat Anti-Rabbit IgG (H+L) Antibody 430/540 100 µg
500 µg
300
600
180
360

L108A
L108B
Andy Fluor™ 430 Goat Anti-Human IgG (H+L) Antibody 430/540 100 µg
500 µg
300
600
180
360

L109A
L109B
Andy Fluor™ 488 Goat Anti-Mouse IgG (H+L) Antibody 495/520 100 µg
500 µg
300
600
180
360

L110A
L110B
Andy Fluor™ 488 Goat Anti-Rabbit IgG (H+L) Antibody 495/520 100 µg
500 µg
300
600
180
360

L111A
L111B
Andy Fluor™ 488 Goat Anti-Human IgG (H+L) Antibody 495/520 100 µg
500 µg
300
600
180
360

L112A
L112B
Andy Fluor™ 488 Rabbit Anti-Goat IgG (H+L) Antibody 495/520 100 µg
500 µg
300
600
180
360

L113A
L113B
Andy Fluor™ 555 Goat Anti-Mouse IgG (H+L) Antibody 553/565 100 µg
500 µg
300
600
180
360

L114A
L114B
Andy Fluor™ 555 Goat Anti-Rabbit IgG (H+L) Antibody 553/565 100 µg
500 µg
300
600
180
360

L115A
L115B
Andy Fluor™ 555 Goat Anti-Human IgG (H+L) Antibody 553/565 100 µg
500 µg
300
600
180
360

L116A
L116B
Andy Fluor™ 568 Goat Anti-Mouse IgG (H+L) Antibody 578/605 100 µg
500 µg
300
600
180
360

L117A
L117B
Andy Fluor™ 568 Goat Anti-Rabbit IgG (H+L) Antibody 578/605 100 µg
500 µg
300
600
180
360

L118A
L118B
Andy Fluor™ 568 Goat Anti-Human IgG (H+L) Antibody 578/605 100 µg
500 µg
300
600
180
360

L119A
L119B
Andy Fluor™ 594 Goat Anti-Mouse IgG (H+L) Antibody 590/615 100 µg
500 µg
300
600
180
360

L120A
L120B
Andy Fluor™ 594 Goat Anti-Rabbit IgG (H+L) Antibody 590/615 100 µg
500 µg
300
600
180
360

L121A
L121B
Andy Fluor™ 594 Goat Anti-Human IgG (H+L) Antibody 590/615 100 µg
500 µg
300
600
180
360

L125A
L125B
Andy Fluor™ 647 Goat Anti-Mouse IgG (H+L) Antibody 650/665 100 µg
500 µg
300
600
180
360

L126A
L126B
Andy Fluor™ 647 Goat Anti-Rabbit IgG (H+L) Antibody 650/665 100 µg
500 µg
300
600
180
360

L127A
L127B
Andy Fluor™ 647 Goat Anti-Human IgG (H+L) Antibody 650/665 100 µg
500 µg
300
600
180
360

L128A
L128B
Andy Fluor™ 680 Goat Anti-Mouse IgG (H+L) Antibody: 680/700 100 µg
500 µg
300
600
180
360

L129A
L129B
Andy Fluor™ 680 Goat Anti-Rabbit IgG (H+L) Antibody 680/700 100 µg
500 µg
300
600
180
360

L130A
L130B
Andy Fluor™ 680 Goat Anti-Human IgG (H+L) Antibody 680/700 100 µg
500 µg
300
600
180
360

L131A
L131B
Andy Fluor™ 750 Goat Anti-Mouse IgG (H+L) Antibody 750/770 100 µg
500 µg
300
600
180
360

L132A
L132B
Andy Fluor™ 750 Goat Anti-Rabbit IgG (H+L) Antibody 750/770 100 µg
500 µg
300
600
180
360

L133A
L133B
Andy Fluor™ 750 Goat Anti-Human IgG (H+L) Antibody 750/770 100 µg
500 µg
300
600
180
360

 

 

Cy 标记二抗

购买 货号 产品名称 激发/发射波长 (nm) 规格 价格¥ 促销价¥

L134A
L134B
Cy® 3 Goat Anti-Mouse IgG (H+L) Antibody 550/565 100 µg
500 µg
300
600
180
360

L135A
L135B
Cy® 3 Goat Anti-Rabbit IgG (H+L) Antibody 550/565 100 µg
500 µg
300
600
180
360

L136A
L136B
Cy® 3 Goat Anti-Human IgG (H+L) Antibody 550/565 100 µg
500 µg
300
600
180
360

L137A
L137B
Cy® 5 Goat Anti-Mouse IgG (H+L) Antibody 650/667 100 µg
500 µg
300
600
180
360

L138A
L138B
Cy® 5 Goat Anti-Rabbit IgG (H+L) Antibody 650/667 100 µg
500 µg
300
600
180
360

L139A
L139B
Cy® 5 Goat Anti-Human IgG (H+L) Antibody 650/667 100 µg
500 µg
300
600
180
360

L140A
L140B
Cy® 5.5 Goat Anti-Mouse IgG (H+L) Antibody 680/700 100 µg
500 µg
300
600
180
360

L141A
L141B
Cy® 5.5 Goat Anti-Rabbit IgG (H+L) Antibody 680/700 100 µg
500 µg
300
600
180
360

L142A
L142B
Cy® 5.5 Goat Anti-Human IgG (H+L) Antibody 680/700 100 µg
500 µg
300
600
180
360

L143A
L143B
Cy® 7 Goat Anti-Mouse IgG (H+L) Antibody 750/770 100 µg
500 µg
300
600
180
360

L144A
L144B
Cy® 7 Goat Anti-Rabbit IgG (H+L) Antibody 750/770 100 µg
500 µg
300
600
180
360

L145A
L145B
Cy® 7 Goat Anti-Human IgG (H+L) Antibody 750/770 100 µg
500 µg
300
600
180
360

 

 

FITC 标记二抗

购买 货号 产品名称 激发/发射波长(nm) 规格 价格¥ 促销价¥

L146A
L146B
FITC Goat Anti-Mouse IgG (H+L) Antibody 495/518 100 µg
500 µg
150
300
90
180

L147A
L147B
FITC Goat Anti-Rabbit IgG (H+L) Antibody 495/518 100 µg
500 µg
150
300
90
180

L148A
L148B
FITC Goat Anti-Human IgG (H+L) Antibody 495/518 100 µg
500 µg
150
300
90
180

 

 

Biotin 标记二抗

购买 货号 产品名称 激发/发射波长(nm) 规格 价格¥ 促销价¥

L149A
L149B
Biotin Goat Anti-Mouse IgG (H+L) Antibody 100 µg
500 µg
150
300
90
180

L150A
L150B
Biotin Goat Anti-Rabbit IgG (H+L) Antibody 100 µg
500 µg
150
300
90
180

L151A
L151B
Biotin Goat Anti-Human IgG (H+L) Antibody 100 µg
500 µg
150
300
90
180

 

 

HRP 标记二抗

购买 货号 产品名称 激发/发射波长(nm) 规格 价格¥ 促销价¥

L152A
L152B
HRP Goat Anti-Mouse IgG (H+L) Antibody 50 µg
250 µg
200
400
120
240

L153A
L153B
HRP Goat Anti-Rabbit IgG (H+L) Antibody 50 µg
250 µg
200
400
120
240

L154A
L154B
HRP Goat Anti-Human IgG (H+L) Antibody 50 µg
250 µg
200
400
120
240

 

 

链霉亲和素耦合物

购买 货号 产品名称 激发/发射波长(nm) 规格 价格¥ 促销价¥

L155A
L155B
Andy Fluor™ 350 Streptavidin 345/440 100 µg
500 µg
300
600
180
360

L156A
L156B
Andy Fluor™ 488 Streptavidin 495/520 100 µg
500 µg
300
600
180
360

L157A
L157B
Andy Fluor™ 555 Streptavidin 553/565 100 µg
500 µg
300
600
180
360

L158A
L158B
Andy Fluor™ 594 Streptavidin 590/615 100 µg
500 µg
300
600
180
360

L159A
L159B
Andy Fluor™ 647 Streptavidin 650/665 100 µg
500 µg
300
600
180
360

L160A
L160B
Cy®3 Streptavidin 555/568 100 µg
500 µg
300
600
180
360

L161A
L161B
Cy®5 Streptavidin 650/665 100 µg
500 µg
300
600
180
360