Vector Datasheet

EZ004 Datasheet

Vector Information

Catalog No.: EZ004

Description: Vector clone.

Whole Plasmid Size: 8873 bp
Vector: pEZ-Lv195
Antibiotic: Ampicillin	Stable Selection Marker: PUROMYCIN
Suggested Sequencing Primers: Forward: 5'-GTTTCGTTTTCTGTTCTGC-3' Reverse: 5'-CTGGAATAGCTCAGAGGC-3'

Vector Information for EZ004

Restriction Enzyme Information for EZ004

Table 1. Restriction Enzymes That Do Not Cleave EZ004

Acc65I

AfeI

ApaI

BclI

BlpI

BsaAI

BstBI

ClaI

EcoRV

HpaI

KpnI

NdeI

PacI

PmeI

PmlI

PshAI

PspOMI

SbfI

SfiI

SgrAI

SnaBI

SpeI

SwaI

XbaI

Table 2. Restriction Enzymes That Cleave EZ004 Once


AasI	2617	Acc16I	3621	Acc36I	8730	AccIII	7623	AdeI	980	AgeI	396	AhdI	3399	AjiI	8520
AleI	5957	Aor13HI	7623	AscI	387	AsiGI	396	AsiSI	6640	AspA2I	330	AspEI	3399	AspI	451
AsuNHI	2121	AviII	3621	AvrII	330	BaeI	4739	BamHI	7377	BfuAI	8730	BlnI	330	BmeRI	3399
BmgBI	8520	BmtI	2125	BsaBI	8499	Bse8I	8499	BseAI	7623	BseJI	8499	BshTI	396	BsiWI	465
Bsp13I	7623	Bsp68I	5209	BspEI	7623	BspMI	8730	BspOI	2125	BssNAI	8150	Bst1107I	8150	BstEII	543
BstPI	543	BstXI	8603	BstZ17I	8150	BtrI	8520	BtuMI	5209	BveI	8730	CciNI	7307	CpoI	525
CspAI	396	CspCI	6959	CspI	525	DraIII	980	DrdI	2617	DriI	3399	DseDI	2617	Eam1105I	3399
Eco91I	543	EcoO65I	543	FseI	5526	FspI	3621	KflI	6314	Kpn2I	7623	MfeI	6712	MluI	1009
MroI	7623	MunI	6712	NheI	2121	NotI	7307	NruI	5209	NsbI	3621	OliI	5957	PaeR7I	1
PalAI	387	PasI	7859	PciI	2509	Pfl23II	465	PflFI	451	PinAI	396	PscI	2509	PspEI	543
PspLI	465	PsyI	451	RgaI	6640	RigI	5526	RruI	5209	Rsr2I	525	RsrII	525	SfaAI	6640
Sfr274I	1	SgfI	6640	SgrDI	4320	SgsI	387	SlaI	1	StrI	1	Tth111I	451	XcmI	4545
XhoI	1	XmaJI	330

Table 3. Restriction Enzymes That Cleave EZ004 Twice


AanI	2085	AanI	4358	AccB7I	1263	AccB7I	7859	AflII	1933	AflII	4893	AflIII	1009	AflIII	2509
ArsI	7017	ArsI	7894	AssI	3879	AssI	8044	AxyI	1743	AxyI	6922	BasI	1263	BasI	7859
BauI	2682	BauI	4063	BbsI	1550	BbsI	1848	BbvCI	5804	BbvCI	8340	BcgI	850	BcgI	3904
BfrI	1933	BfrI	4893	BmcAI	3879	BmcAI	8044	BpiI	1550	BpiI	1848	BpuAI	1550	BpuAI	1848
Bse21I	1743	Bse21I	6922	BsmBI	801	BsmBI	7851	BspHI	3226	BspHI	4234	BspQI	2393	BspQI	5478
BspTI	1933	BspTI	4893	BssSI	2682	BssSI	4063	Bst2BI	2682	Bst2BI	4063	BstAFI	1933	BstAFI	4893
BstV2I	1550	BstV2I	1848	Bsu36I	1743	Bsu36I	6922	CciI	3226	CciI	4234	Cfr42I	623	Cfr42I	1525
CsiI	97	CsiI	976	DraII	1747	DraII	6314	Eco147I	329	Eco147I	740	Eco81I	1743	Eco81I	6922
EcoO109I	1747	EcoO109I	6314	EcoRI	6622	EcoRI	7627	EcoT22I	80	EcoT22I	152	Esp3I	801	Esp3I	7851
KspI	623	KspI	1525	LguI	2393	LguI	5478	MabI	97	MabI	976	Mph1103I	80	Mph1103I	152
MspCI	1933	MspCI	4893	NsiI	80	NsiI	152	PagI	3226	PagI	4234	PceI	329	PceI	740
PciSI	2393	PciSI	5478	PflMI	1263	PflMI	7859	PfoI	884	PfoI	1295	PpuMI	1747	PpuMI	6314
PsiI	2085	PsiI	4358	Psp5II	1747	Psp5II	6314	PspPPI	1747	PspPPI	6314	PstI	1627	PstI	8741
RcaI	3226	RcaI	4234	SacII	623	SacII	1525	SapI	2393	SapI	5478	ScaI	3879	ScaI	8044
SexAI	97	SexAI	976	Sfr303I	623	Sfr303I	1525	SgrBI	623	SgrBI	1525	SseBI	329	SseBI	740
SspI	4203	SspI	7939	StuI	329	StuI	740	Van91I	1263	Van91I	7859	Vha464I	1933	Vha464I	4893
ZrmI	3879	ZrmI	8044	Zsp2I	80	Zsp2I	152

Table 4. Restriction Enzymes That Cleave EZ004 Three Times


Asp700I	1618	Asp700I	3998	Asp700I	5220	BciVI	1091	BciVI	2712	BciVI	4236	BfuI	1091	BfuI	2712
BfuI	4236	BglI	625	BglI	756	BglI	3519	BpvUI	3769	BpvUI	4721	BpvUI	6640	BsaXI	787
BsaXI	2384	BsaXI	6228	BseYI	51	BseYI	123	BseYI	2813	Bsp1407I	7188	Bsp1407I	8459	Bsp1407I	8860
Bsp19I	237	Bsp19I	1430	Bsp19I	7928	BsrGI	7188	BsrGI	8459	BsrGI	8860	BstAUI	7188	BstAUI	8459
BstAUI	8860	BstENI	5546	BstENI	6730	BstENI	6921	BsuI	1091	BsuI	2712	BsuI	4236	Cfr9I	351
Cfr9I	458	Cfr9I	8484	Ecl136II	1905	Ecl136II	4865	Ecl136II	5056	Eco53kI	1905	Eco53kI	4865	Eco53kI	5056
EcoICRI	1905	EcoICRI	4865	EcoICRI	5056	EcoNI	5546	EcoNI	6730	EcoNI	6921	GsaI	55	GsaI	127
GsaI	2817	KroI	1534	KroI	5522	KroI	7071	MroNI	1534	MroNI	5522	MroNI	7071	MroXI	1618
MroXI	3998	MroXI	5220	MvrI	3769	MvrI	4721	MvrI	6640	NaeI	1536	NaeI	5524	NaeI	7073
NcoI	237	NcoI	1430	NcoI	7928	NgoMIV	1534	NgoMIV	5522	NgoMIV	7071	PaeI	78	PaeI	150
PaeI	4693	PdiI	1536	PdiI	5524	PdiI	7073	PdmI	1618	PdmI	3998	PdmI	5220	Ple19I	3769
Ple19I	4721	Ple19I	6640	Psp124BI	1907	Psp124BI	4867	Psp124BI	5058	PvuI	3769	PvuI	4721	PvuI	6640
SacI	1907	SacI	4867	SacI	5058	SmaI	353	SmaI	460	SmaI	8486	SphI	78	SphI	150
SphI	4693	SstI	1907	SstI	4867	SstI	5058	TspMI	351	TspMI	458	TspMI	8484	XagI	5546
XagI	6730	XagI	6921	XmaI	351	XmaI	458	XmaI	8484	XmnI	1618	XmnI	3998	XmnI	5220