Vector Datasheet

EZ006 Datasheet

Vector Information

Catalog No.: EZ006

Description: Vector clone.

Whole Plasmid Size: 10262 bp
Vector: pEZ-Lv201
Antibiotic: Ampicillin	Stable Selection Marker: Puromycin
Suggested Sequencing Primers: Forward: 5'-GCGGTAGGCGTGTACGGT-3' Reverse: 5'-CTGGAATAGCTCAGAGGC-3'

Vector Information for EZ006

Restriction Enzyme Information for EZ006

Table 1. Restriction Enzymes That Do Not Cleave EZ006

AfeI

BclI

BlpI

BstBI

BstZ17I

ClaI

EcoRV

HpaI

MfeI

PacI

PmeI

PshAI

SbfI

SfiI

SgfI

SgrAI

SpeI

SwaI

XbaI

Table 2. Restriction Enzymes That Cleave EZ006 Once


AarI	3178	AasI	5653	Acc16I	6657	Acc65I	3280	AccIII	444	AcvI	3155	AgeI	2090	AhdI	6435
Aor13HI	444	ApaI	2956	AscI	2081	AsiGI	2090	Asp718I	3280	AspEI	6435	AspI	3487	AsuNHI	5157
AviII	6657	AxyI	4779	BaeI	7775	BamHI	198	BbrPI	3155	BmeRI	6435	BmtI	5161	BsaBI	1320
Bse21I	4779	Bse8I	1320	BseAI	444	BseJI	1320	BshTI	2090	BsiWI	3501	Bsp120I	2952	Bsp13I	444
Bsp68I	8245	BspEI	444	BspOI	5161	BstEII	3579	BstENI	8582	BstPI	3579	BstSNI	9993	BstXI	1424
Bsu36I	4779	BtuMI	8245	CpoI	3561	CspAI	2090	CspCI	10029	CspI	3561	DrdI	5653	DriI	6435
DseDI	5653	Eam1105I	6435	Eco105I	9993	Eco72I	3155	Eco81I	4779	Eco91I	3579	EcoNI	8582	EcoO65I	3579
FauNDI	9887	FseI	8562	FspI	6657	KflI	9350	Kpn2I	444	KpnI	3284	MluI	4045	MroI	444
NdeI	9887	NheI	5157	NruI	8245	NsbI	6657	PaeR7I	1695	PalAI	2081	PasI	680	Pfl23II	3501
PflFI	3487	PinAI	2090	PmaCI	3155	PmlI	3155	PspCI	3155	PspEI	3579	PspLI	3501	PspOMI	2952
PstI	4663	PsyI	3487	RigI	8562	RruI	8245	Rsr2I	3561	RsrII	3561	Sfr274I	1695	SgrDI	7356
SgsI	2081	SlaI	1695	SnaBI	9993	StrI	1695	Tth111I	3487	XagI	8582	XcmI	7581	XhoI	1695

Table 3. Restriction Enzymes That Cleave EZ006 Twice


AanI	5121	AanI	7394	Acc36I	1551	Acc36I	3178	AdeI	3202	AdeI	4016	AflII	4969	AflII	7929
AjiI	1341	AjiI	3382	ArsI	715	ArsI	2397	AspA2I	2024	AspA2I	2990	AssI	865	AssI	6915
AvrII	2024	AvrII	2990	BbvCI	1161	BbvCI	8840	BfrI	4969	BfrI	7929	BfuAI	1551	BfuAI	3178
BlnI	2024	BlnI	2990	BmcAI	865	BmcAI	6915	BmgBI	1341	BmgBI	3382	BpvUI	6805	BpvUI	7757
BsaAI	3155	BsaAI	9993	BspHI	6262	BspHI	7270	BspMI	1551	BspMI	3178	BspQI	5429	BspQI	8514
BspTI	4969	BspTI	7929	BstAFI	4969	BstAFI	7929	BstBAI	3155	BstBAI	9993	BtrI	1341	BtrI	3382
BveI	1551	BveI	3178	CciI	6262	CciI	7270	CciNI	128	CciNI	2825	Cfr42I	3659	Cfr42I	4561
CsiI	1791	CsiI	4012	DraIII	3202	DraIII	4016	Eco147I	2023	Eco147I	3776	EcoRI	448	EcoRI	9658
EcoT22I	1774	EcoT22I	1846	KspI	3659	KspI	4561	LguI	5429	LguI	8514	MabI	1791	MabI	4012
Mph1103I	1774	Mph1103I	1846	MspCI	4969	MspCI	7929	MvrI	6805	MvrI	7757	NotI	128	NotI	2825
NsiI	1774	NsiI	1846	PagI	6262	PagI	7270	PceI	2023	PceI	3776	PciI	3329	PciI	5545
PciSI	5429	PciSI	8514	Ple19I	6805	Ple19I	7757	Ppu21I	3155	Ppu21I	9993	PpuMI	4783	PpuMI	9350
PscI	3329	PscI	5545	PsiI	5121	PsiI	7394	Psp5II	4783	Psp5II	9350	PspPPI	4783	PspPPI	9350
PvuI	6805	PvuI	7757	RcaI	6262	RcaI	7270	SacII	3659	SacII	4561	SapI	5429	SapI	8514
ScaI	865	ScaI	6915	SexAI	1791	SexAI	4012	Sfr303I	3659	Sfr303I	4561	SgrBI	3659	SgrBI	4561
SseBI	2023	SseBI	3776	SspI	760	SspI	7239	StuI	2023	StuI	3776	Vha464I	4969	Vha464I	7929
XmaJI	2024	XmaJI	2990	ZrmI	865	ZrmI	6915	Zsp2I	1774	Zsp2I	1846

Table 4. Restriction Enzymes That Cleave EZ006 Three Times


AccB7I	680	AccB7I	3292	AccB7I	4299	BasI	680	BasI	3292	BasI	4299	BauI	2283	BauI	5718
BauI	7099	BcgI	2193	BcgI	3886	BcgI	6940	BciVI	4127	BciVI	5748	BciVI	7272	BfuI	4127
BfuI	5748	BfuI	7272	BsaXI	3823	BsaXI	5420	BsaXI	9264	BseYI	1745	BseYI	1817	BseYI	5849
BsmBI	672	BsmBI	3426	BsmBI	3837	BssSI	2283	BssSI	5718	BssSI	7099	Bst2BI	2283	Bst2BI	5718
Bst2BI	7099	BsuI	4127	BsuI	5748	BsuI	7272	Cfr9I	1305	Cfr9I	2045	Cfr9I	3494	DinI	3654
DinI	3785	DinI	8051	Ecl136II	4941	Ecl136II	7901	Ecl136II	8092	Eco53kI	4941	Eco53kI	7901	Eco53kI	8092
EcoICRI	4941	EcoICRI	7901	EcoICRI	8092	EgeI	3654	EgeI	3785	EgeI	8051	EheI	3654	EheI	3785
EheI	8051	Esp3I	672	Esp3I	3426	Esp3I	3837	GsaI	1749	GsaI	1821	GsaI	5853	KasI	3652
KasI	3783	KasI	8049	KroI	1436	KroI	4570	KroI	8558	Mly113I	3653	Mly113I	3784	Mly113I	8050
MroNI	1436	MroNI	4570	MroNI	8558	NaeI	1438	NaeI	4572	NaeI	8560	NarI	3653	NarI	3784
NarI	8050	NgoMIV	1436	NgoMIV	4570	NgoMIV	8558	PaeI	1772	PaeI	1844	PaeI	7729	PdiI	1438
PdiI	4572	PdiI	8560	PflMI	680	PflMI	3292	PflMI	4299	PfoI	2383	PfoI	3920	PfoI	4331
PluTI	3656	PluTI	3787	PluTI	8053	Psp124BI	4943	Psp124BI	7903	Psp124BI	8094	PvuII	348	PvuII	4813
PvuII	5369	SacI	4943	SacI	7903	SacI	8094	SalI	3553	SalI	5171	SalI	7356	SfoI	3654
SfoI	3785	SfoI	8051	SmaI	1307	SmaI	2047	SmaI	3496	SphI	1772	SphI	1844	SphI	7729
SspDI	3652	SspDI	3783	SspDI	8049	SstI	4943	SstI	7903	SstI	8094	TspMI	1305	TspMI	2045
TspMI	3494	Van91I	680	Van91I	3292	Van91I	4299	XmaI	1305	XmaI	2045	XmaI	3494