Vector Datasheet

EZ008 Datasheet

Vector Information

Catalog No.: EZ008

Description: Vector clone.

Whole Plasmid Size: 7080 bp
Vector: pEZ-M13
Antibiotic: Ampicillin	Stable Selection Marker: Neomycin
Suggested Sequencing Primers: Forward: 5'-GCGGTAGGCGTGTACGGT-3' Reverse: 5'-GTGGCACCTTCCAGGGTC-3'

Vector Information for EZ008

Restriction Enzyme Information for EZ008

Table 1. Restriction Enzymes That Do Not Cleave EZ008

Acc65I

AfeI

AflII

AgeI

ApaI

AscI

BaeI

BlpI

BsgI

BsiWI

BstBI

BstEII

Bsu36I

ClaI

EcoNI

EcoO109I

EcoRV

FseI

HpaI

KpnI

PacI

PmeI

PmlI

PpuMI

PshAI

PspOMI

SacII

SanDI

SbfI

SfiI

SgfI

SgrAI

SwaI

TliI

XcmI

XhoI

XmnI

Table 2. Restriction Enzymes That Cleave EZ008 Once


AccB7I	6062	AccIII	5826	AdeI	568	AhdI	3547	AhlI	4719	AjiI	6735	AloI	636	Aor13HI	5826
ArsI	6097	AspA2I	1091	AspEI	3547	AspI	1419	AsuNHI	22	AvrII	1091	BasI	6062	BbsI	254
BbvCI	6555	BcgI	4052	BcuI	4719	BlnI	1091	BmeRI	3547	BmgBI	6735	BmtI	26	BpiI	254
BpuAI	254	BpvUI	3917	BseAI	5826	BseRI	1087	BsmBI	6054	Bsp13I	5826	BspEI	5826	BspOI	26
BstSNI	5060	BstV2I	254	BstXI	6818	BtrI	6735	CciNI	5510	CpoI	1817	CsiI	858	CspCI	5096
CspI	1817	DinI	1302	DraIII	568	DriI	3547	Eam1105I	3547	Ecl136II	5286	Eco105I	5060	Eco147I	1090
Eco53kI	5286	EcoICRI	5286	EcoRI	5830	EgeI	1302	EheI	1302	Esp3I	6054	FauNDI	4954	HindIII	5372
KasI	1300	Kpn2I	5826	MabI	858	MfeI	4631	MluI	4698	Mly113I	1301	MroI	5826	MunI	4631
MvrI	3917	NarI	1301	NdeI	4954	NheI	22	NotI	5510	PasI	6062	PceI	1090	PciI	2654
PflFI	1419	PflMI	6062	PfoI	2078	Ple19I	3917	PluTI	1304	PscI	2654	Psp124BI	5288	PsyI	1419
PvuI	3917	Rsr2I	1817	RsrII	1817	SacI	5288	SexAI	858	SfoI	1302	SgrDI	4468	SnaBI	5060
SpeI	4719	SseBI	1090	SspDI	1300	SstI	5288	StuI	1090	Tth111I	1419	Van91I	6062	XbaI	13
XmaJI	1091

Table 3. Restriction Enzymes That Cleave EZ008 Twice


AanI	693	AanI	2162	Acc16I	1403	Acc16I	3769	AclI	3773	AclI	5917	AflIII	2654	AflIII	4698
Ama87I	1112	Ama87I	6699	AssI	4027	AssI	6247	AvaI	1112	AvaI	6699	AviII	1403	AviII	3769
BamHI	5580	BamHI	6283	BglII	4482	BglII	6277	BmcAI	4027	BmcAI	6247	BmeT110I	1112	BmeT110I	6699
BsaXI	634	BsaXI	2529	BsePI	1698	BsePI	6324	BseX3I	1207	BseX3I	5510	BsiHKCI	1112	BsiHKCI	6699
BsoBI	1112	BsoBI	6699	Bsp68I	1985	Bsp68I	4678	BspHI	3374	BspHI	4382	BssHII	1698	BssHII	6324
BssNAI	2275	BssNAI	6365	Bst1107I	2275	Bst1107I	6365	BstAPI	836	BstAPI	908	BstZ17I	2275	BstZ17I	6365
BstZI	1207	BstZI	5510	BtuMI	1985	BtuMI	4678	CciI	3374	CciI	4382	Cfr9I	1112	Cfr9I	6699
EagI	1207	EagI	5510	EclXI	1207	EclXI	5510	Eco52I	1207	Eco52I	5510	Eco88I	1112	Eco88I	6699
EcoT22I	841	EcoT22I	913	FalI	1609	FalI	1641	FspI	1403	FspI	3769	Mph1103I	841	Mph1103I	913
NruI	1985	NruI	4678	NsbI	1403	NsbI	3769	NsiI	841	NsiI	913	PagI	3374	PagI	4382
PauI	1698	PauI	6324	PsiI	693	PsiI	2162	Psp1406I	3773	Psp1406I	5917	PstI	1354	PstI	6956
PteI	1698	PteI	6324	RcaI	3374	RcaI	4382	RruI	1985	RruI	4678	ScaI	4027	ScaI	6247
SmaI	1114	SmaI	6701	SspI	4351	SspI	6142	TspMI	1112	TspMI	6699	XmaI	1112	XmaI	6699
ZrmI	4027	ZrmI	6247	Zsp2I	841	Zsp2I	913

Table 4. Restriction Enzymes That Cleave EZ008 Three Times


AasI	612	AasI	1328	AasI	2762	AlwNI	3070	AlwNI	4550	AlwNI	6609	AvaII	1817	AvaII	3685
AvaII	3907	BclI	52	BclI	1142	BclI	5377	Bme18I	1817	Bme18I	3685	Bme18I	3907	Bpu10I	4650
Bpu10I	5603	Bpu10I	6555	BsaAI	565	BsaAI	1605	BsaAI	5060	BsaBI	1160	BsaBI	6282	BsaBI	6714
BsaI	3608	BsaI	5346	BsaI	6839	Bse8I	1160	Bse8I	6282	Bse8I	6714	BseJI	1160	BseJI	6282
BseJI	6714	BsmI	2223	BsmI	5823	BsmI	6230	Bso31I	3608	Bso31I	5346	Bso31I	6839	Bsp1407I	5391
Bsp1407I	6674	Bsp1407I	7076	BsrGI	5391	BsrGI	6674	BsrGI	7076	BstAUI	5391	BstAUI	6674	BstAUI	7076
BstBAI	565	BstBAI	1605	BstBAI	5060	CaiI	3070	CaiI	4550	CaiI	6609	DrdI	612	DrdI	1328
DrdI	2762	DseDI	612	DseDI	1328	DseDI	2762	Eco31I	3608	Eco31I	5346	Eco31I	6839	Eco47I	1817
Eco47I	3685	Eco47I	3907	FbaI	52	FbaI	1142	FbaI	5377	KroI	460	KroI	1801	KroI	2086
Ksp22I	52	Ksp22I	1142	Ksp22I	5377	MroNI	460	MroNI	1801	MroNI	2086	Mva1269I	2223	Mva1269I	5823
Mva1269I	6230	NaeI	462	NaeI	1803	NaeI	2088	NgoMIV	460	NgoMIV	1801	NgoMIV	2086	NmeAIII	1058
NmeAIII	1526	NmeAIII	3696	PctI	2223	PctI	5823	PctI	6230	PdiI	462	PdiI	1803	PdiI	2088
Ppu21I	565	Ppu21I	1605	Ppu21I	5060	PstNI	3070	PstNI	4550	PstNI	6609	SalI	2280	SalI	4468
SalI	6958	VpaK11BI	1817	VpaK11BI	3685	VpaK11BI	3907