Vector Datasheet

EZ014 Datasheet

Vector Information

Catalog No.: EZ014

Description: Custom clone

Whole Plasmid Size: 7212 bp
Vector: pEZ-B01
Antibiotic: Ampicillin	Stable Selection Marker: N/A
Suggested Sequencing Primers: Forward: 5'-TAATACGACTCACTATAGGG-3' Reverse: 5'-TTCACTTCTGAGTTCGGCATG-3'

Vector Information for EZ014

Restriction Enzyme Information for EZ014

Table 1. Restriction Enzymes That Do Not Cleave EZ014

AatII

Acc65I

AflII

AgeI

AscI

BaeI

BglII

BplI

BseRI

BsiWI

Bsu36I

Ecl136II

EcoRV

FseI

HindIII

KpnI

MfeI

NheI

NruI

PmeI

PmlI

PshAI

PstI

RsrII

SacI

SacII

SalI

SanDI

SbfI

SexAI

SfiI

SgfI

SnaBI

SpeI

StuI

SwaI

XmnI

Table 2. Restriction Enzymes That Cleave EZ014 Once


AanI	932	Acc16I	1439	Acc36I	7096	AccI	3054	AdeI	807	AhdI	1217	AjiI	6886	AloI	875
ApaI	4483	ArsI	6260	AspA2I	75	AspEI	1217	AspI	3080	AsuII	5542	AviII	1439	AvrII	75
BamHI	5743	BarI	5497	BbvCI	6706	BclI	4672	BfuAI	7096	BglI	1337	BlnI	75	BlpI	479
BmeRI	1217	BmgBI	6886	Bpu1102I	479	Bpu14I	5542	BpvUI	1587	Bsa29I	5411	BseCI	5411	BseX3I	5673
BshVI	5411	Bsp119I	5542	Bsp120I	4479	Bsp1720I	479	Bsp19I	6294	BspDI	5411	BspMI	7096	BspT104I	5542
BssNAI	3055	Bst1107I	3055	BstAPI	5010	BstBI	5542	BstEII	4504	BstENI	5154	BstPI	4504	BstZ17I	3055
BstZI	5673	Bsu15I	5411	BtrI	6886	BveI	7096	CciNI	5673	CelII	479	Cfr9I	6850	ClaI	5411
DraIII	807	DriI	1217	EagI	5673	Eam1105I	1217	EclXI	5673	Eco52I	5673	Eco91I	4504	EcoNI	5154
EcoO65I	4504	EcoRI	5993	EcoT22I	5524	FauNDI	5518	FbaI	4672	FblI	3054	FspI	1439	HpaI	4184
Ksp22I	4672	KspAI	4184	MluI	4686	Mph1103I	5524	MvrI	1587	NcoI	6294	NdeI	5518	NotI	5673
NsbI	1439	NsiI	5524	NspV	5542	PacI	61	PaeI	5219	PaeR7I	28	PasI	6225	PciI	2822
PflFI	3080	Ple19I	1587	PpuMI	3817	PscI	2822	PsiI	932	Psp5II	3817	PspEI	4504	PspOMI	4479
PspPPI	3817	PsyI	3080	PvuI	1587	Sfr274I	28	SfuI	5542	SgrAI	5367	SlaI	28	SmaI	6852
SphI	5219	StrI	28	TspMI	6850	Tth111I	3080	XagI	5154	XbaI	5478	XhoI	28	XmaI	6850
XmaJI	75	XmiI	3054	Zsp2I	5524

Table 3. Restriction Enzymes That Cleave EZ014 Twice


AccB7I	5111	AccB7I	6225	AcuI	1884	AcuI	2280	AfeI	3572	AfeI	5285	AflIII	2822	AflIII	4686
AlwNI	2413	AlwNI	6760	Ama87I	28	Ama87I	6850	Aor51HI	3572	Aor51HI	5285	AssI	1697	AssI	6410
AvaI	28	AvaI	6850	BasI	5111	BasI	6225	BauI	1881	BauI	2649	BmcAI	1697	BmcAI	6410
BmeT110I	28	BmeT110I	6850	BsaAI	804	BsaAI	3074	BsaI	187	BsaI	1278	BsaXI	873	BsaXI	4048
BsePI	4275	BsePI	6475	BsiHKCI	28	BsiHKCI	6850	BsmI	5986	BsmI	6393	Bso31I	187	Bso31I	1278
BsoBI	28	BsoBI	6850	BspQI	1825	BspQI	2939	BssHII	4275	BssHII	6475	BssSI	1881	BssSI	2649
Bst2BI	1881	Bst2BI	2649	BstBAI	804	BstBAI	3074	BstDSI	5249	BstDSI	6294	BtgI	5249	BtgI	6294
CaiI	2413	CaiI	6760	Eco31I	187	Eco31I	1278	Eco47III	3572	Eco47III	5285	Eco57I	1884	Eco57I	2280
Eco88I	28	Eco88I	6850	HincII	4184	HincII	6573	HindII	4184	HindII	6573	LguI	1825	LguI	2939
Mva1269I	5986	Mva1269I	6393	NmeAIII	1366	NmeAIII	4487	PauI	4275	PauI	6475	PciSI	1825	PciSI	2939
PctI	5986	PctI	6393	PflMI	5111	PflMI	6225	PfoI	3179	PfoI	5118	Ppu21I	804	Ppu21I	3074
PstNI	2413	PstNI	6760	PteI	4275	PteI	6475	SapI	1825	SapI	2939	ScaI	1697	ScaI	6410
SspI	1012	SspI	6305	Van91I	5111	Van91I	6225	ZrmI	1697	ZrmI	6410

Table 4. Restriction Enzymes That Cleave EZ014 Three Times


AasI	851	AasI	2720	AasI	3135	AccIII	556	AccIII	3633	AccIII	5989	Aor13HI	556	Aor13HI	3633
Aor13HI	5989	BalI	6260	BalI	6877	BalI	6973	BbsI	3704	BbsI	4201	BbsI	4540	BcgI	1722
BcgI	3217	BcgI	4400	BpiI	3704	BpiI	4201	BpiI	4540	Bpu10I	3717	Bpu10I	5766	Bpu10I	6706
BpuAI	3704	BpuAI	4201	BpuAI	4540	BseAI	556	BseAI	3633	BseAI	5989	BseYI	2518	BseYI	4152
BseYI	4287	BsmBI	3181	BsmBI	4071	BsmBI	6217	Bsp13I	556	Bsp13I	3633	Bsp13I	5989	Bsp1407I	14
Bsp1407I	5554	Bsp1407I	6825	BspEI	556	BspEI	3633	BspEI	5989	BsrGI	14	BsrGI	5554	BsrGI	6825
BssT1I	75	BssT1I	501	BssT1I	6294	BstAUI	14	BstAUI	5554	BstAUI	6825	BstV2I	3704	BstV2I	4201
BstV2I	4540	DraII	506	DraII	3817	DraII	5254	DrdI	851	DrdI	2720	DrdI	3135	DseDI	851
DseDI	2720	DseDI	3135	Eco130I	75	Eco130I	501	Eco130I	6294	EcoO109I	506	EcoO109I	3817	EcoO109I	5254
EcoT14I	75	EcoT14I	501	EcoT14I	6294	ErhI	75	ErhI	501	ErhI	6294	Esp3I	3181	Esp3I	4071
Esp3I	6217	GsaI	2522	GsaI	4156	GsaI	4291	Kpn2I	556	Kpn2I	3633	Kpn2I	5989	KroI	699
KroI	5376	KroI	6981	MlsI	6260	MlsI	6877	MlsI	6973	MluNI	6260	MluNI	6877	MluNI	6973
MmeI	829	MmeI	2429	MmeI	2613	MroI	556	MroI	3633	MroI	5989	MroNI	699	MroNI	5376
MroNI	6981	MscI	6260	MscI	6877	MscI	6973	Msp20I	6260	Msp20I	6877	Msp20I	6973	NaeI	701
NaeI	5378	NaeI	6983	NgoMIV	699	NgoMIV	5376	NgoMIV	6981	PdiI	701	PdiI	5378	PdiI	6983
StyI	75	StyI	501	StyI	6294	XcmI	4301	XcmI	4319	XcmI	4835