Vector Datasheet

EZ010 Datasheet

Vector Information

Catalog No.: EZ010

Description: Vector clone.

Whole Plasmid Size: 7238 bp
Vector: pEZ-M68
Antibiotic: Ampicillin	Stable Selection Marker: Neomycin
Suggested Sequencing Primers: Forward: 5'-CAGCCTCCGGACTCTAGC-3' Reverse: 5'-TAATACGACTCACTATAGGG-3'

Vector Information for EZ010

Restriction Enzyme Information for EZ010

Table 1. Restriction Enzymes That Do Not Cleave EZ010

Acc65I

AfeI

AgeI

ApaI

AscI

BaeI

BaeI

BlpI

BsgI

Bsu36I

EcoNI

EcoRV

FseI

KpnI

MluI

NruI

PacI

PmeI

PmlI

PpuMI

PshAI

PspOMI

SanDI

SbfI

SgfI

SgrAI

SpeI

SwaI

TliI

XbaI

XcmI

XhoI

XmnI

Table 2. Restriction Enzymes That Cleave EZ010 Once


AccB7I	6220	AdeI	984	AflII	1715	AflIII	4520	AhdI	3632	AjiI	6893	AjuI	367	AloI	1052
Ama87I	6857	ArsI	6255	AspA2I	1667	AspEI	3632	AspI	1775	AsuII	5535	AsuNHI	108	AvaI	6857
AvrII	1667	BarI	119	BasI	6220	BbvCI	6713	BclI	384	BfrI	1715	BlnI	1667	BmeRI	3632
BmeT110I	6857	BmgBI	6893	BmtI	112	Bpu14I	5535	BseRI	1663	BsiHKCI	6857	BsiWI	1789	BsoBI	6857
Bsp119I	5535	BspOI	112	BspQI	4637	BspT104I	5535	BspTI	1715	BssNAI	6523	Bst1107I	6523	BstAFI	1715
BstBI	5535	BstEII	1867	BstPI	1867	BstSNI	5203	BstZ17I	6523	BtrI	6893	CciNI	5668	Cfr42I	1947
Cfr9I	6857	CpoI	1849	CspCI	5239	CspI	1849	DraIII	984	DriI	3632	Eam1105I	3632	Ecl136II	5429
Eco105I	5203	Eco53kI	5429	Eco88I	6857	Eco91I	1867	EcoICRI	5429	EcoO65I	1867	EcoRI	5988	FbaI	384
HindIII	1683	HpaI	490	KroI	876	Ksp22I	384	KspAI	490	KspI	1947	LguI	4637	MfeI	477
MroNI	876	MspCI	1715	MunI	477	NaeI	878	NgoMIV	876	NheI	108	NotI	5668	NspV	5535
PciI	4520	PciSI	4637	PdiI	878	Pfl23II	1789	PflFI	1775	PflMI	6220	PscI	4520	Psp124BI	5431
PspEI	1867	PspLI	1789	PstI	7114	PsyI	1775	Rsr2I	1849	RsrII	1849	SacI	5431	SacII	1947
SalI	7116	SapI	4637	SfiI	1620	Sfr303I	1947	SfuI	5535	SgrBI	1947	SmaI	6859	SnaBI	5203
SstI	5431	TspMI	6857	Tth111I	1775	Van91I	6220	Vha464I	1715	XmaI	6857	XmaJI	1667

Table 3. Restriction Enzymes That Cleave EZ010 Twice


Acc36I	2374	Acc36I	7103	AccI	6522	AccI	7117	AcuI	2966	AcuI	3978	AlwNI	4111	AlwNI	6767
AssI	3151	AssI	6405	BbsI	3032	BbsI	5497	BfuAI	2374	BfuAI	7103	BglII	5529	BglII	6435
BmcAI	3151	BmcAI	6405	BpiI	3032	BpiI	5497	BplI	1602	BplI	1634	Bpu10I	5761	Bpu10I	6713
BpuAI	3032	BpuAI	5497	BpvUI	710	BpvUI	3263	Bsa29I	624	Bsa29I	2437	BsaAI	981	BsaAI	5203
BseCI	624	BseCI	2437	BsePI	2188	BsePI	6482	BseX3I	2181	BseX3I	5668	BshVI	624	BshVI	2437
BspDI	624	BspDI	2437	BspMI	2374	BspMI	7103	BssHII	2188	BssHII	6482	BstBAI	981	BstBAI	5203
BstV2I	3032	BstV2I	5497	BstXI	2377	BstXI	6976	BstZI	2181	BstZI	5668	Bsu15I	624	Bsu15I	2437
BveI	2374	BveI	7103	CaiI	4111	CaiI	6767	ClaI	624	ClaI	2437	CsiI	1434	CsiI	2300
DinI	1942	DinI	2073	EagI	2181	EagI	5668	EclXI	2181	EclXI	5668	Eco147I	1666	Eco147I	2064
Eco52I	2181	Eco52I	5668	Eco57I	2966	Eco57I	3978	EgeI	1942	EgeI	2073	EheI	1942	EheI	2073
FalI	1292	FalI	1324	FauNDI	238	FauNDI	5097	FblI	6522	FblI	7117	KasI	1940	KasI	2071
MabI	1434	MabI	2300	Mly113I	1941	Mly113I	2072	MvrI	710	MvrI	3263	NarI	1941	NarI	2072
NdeI	238	NdeI	5097	PasI	1782	PasI	6220	PauI	2188	PauI	6482	PceI	1666	PceI	2064
PfoI	2208	PfoI	2593	Ple19I	710	Ple19I	3263	PluTI	1944	PluTI	2075	Ppu21I	981	Ppu21I	5203
PstNI	4111	PstNI	6767	PteI	2188	PteI	6482	PvuI	710	PvuI	3263	PvuII	1343	PvuII	5888
ScaI	3151	ScaI	6405	SexAI	1434	SexAI	2300	SfoI	1942	SfoI	2073	SseBI	1666	SseBI	2064
SspDI	1940	SspDI	2071	StuI	1666	StuI	2064	XmiI	6522	XmiI	7117	ZrmI	3151	ZrmI	6405

Table 4. Restriction Enzymes That Cleave EZ010 Three Times


AanI	59	AanI	510	AanI	1109	Acc16I	729	Acc16I	1271	Acc16I	3409	AccIII	1846	AccIII	5514
AccIII	5984	AclI	1194	AclI	3403	AclI	6075	Aor13HI	1846	Aor13HI	5514	Aor13HI	5984	AseI	635
AseI	3457	AseI	4692	AviII	729	AviII	1271	AviII	3409	BamHI	2450	BamHI	5738	BamHI	6441
BauI	2656	BauI	2963	BauI	4347	BcgI	2174	BcgI	2286	BcgI	3128	BciVI	2795	BciVI	4322
BciVI	4759	BfuI	2795	BfuI	4322	BfuI	4759	BsaI	2207	BsaI	3566	BsaI	6997	Bse3DI	3398
Bse3DI	3572	Bse3DI	6007	BseAI	1846	BseAI	5514	BseAI	5984	BseMI	3398	BseMI	3572	BseMI	6007
BseYI	1388	BseYI	1460	BseYI	4216	Bso31I	2207	Bso31I	3566	Bso31I	6997	Bsp13I	1846	Bsp13I	5514
Bsp13I	5984	Bsp1407I	5549	Bsp1407I	6832	Bsp1407I	7234	BspEI	1846	BspEI	5514	BspEI	5984	BsrDI	3398
BsrDI	3572	BsrDI	6007	BsrGI	5549	BsrGI	6832	BsrGI	7234	BssSI	2656	BssSI	2963	BssSI	4347
Bst2BI	2656	Bst2BI	2963	Bst2BI	4347	BstAPI	1412	BstAPI	1484	BstAPI	4703	BstAUI	5549	BstAUI	6832
BstAUI	7234	BsuI	2795	BsuI	4322	BsuI	4759	BtsI	566	BtsI	3213	BtsI	3233	DraII	357
DraII	2652	DraII	4898	Eco31I	2207	Eco31I	3566	Eco31I	6997	EcoO109I	357	EcoO109I	2652	EcoO109I	4898
EcoT22I	1417	EcoT22I	1489	EcoT22I	4920	FspI	729	FspI	1271	FspI	3409	GsaI	1392	GsaI	1464
GsaI	4220	HincII	490	HincII	6580	HincII	7118	HindII	490	HindII	6580	HindII	7118	Kpn2I	1846
Kpn2I	5514	Kpn2I	5984	MmeI	1006	MmeI	4127	MmeI	4311	Mph1103I	1417	Mph1103I	1489	Mph1103I	4920
MroI	1846	MroI	5514	MroI	5984	NsbI	729	NsbI	1271	NsbI	3409	NsiI	1417	NsiI	1489
NsiI	4920	PaeI	22	PaeI	1415	PaeI	1487	PshBI	635	PshBI	3457	PshBI	4692	PsiI	59
PsiI	510	PsiI	1109	Psp1406I	1194	Psp1406I	3403	Psp1406I	6075	SphI	22	SphI	1415	SphI	1487
VspI	635	VspI	3457	VspI	4692	Zsp2I	1417	Zsp2I	1489	Zsp2I	4920