EZ011 Datasheet

Vector Information
Catalog No.:  EZ011
Description:  Vector clone.
Whole Plasmid Size:  7982 bp
Vector:  pEZ-M98 
Antibiotic:    AmpicillinStable Selection Marker: Neomycin

Suggested Sequencing Primers: 
Forward: 5'-GCGGTAGGCGTGTACGGT-3'
Reverse: 5'-CCGGACACGCTGAACTTGT-3'

Vector Information for EZ011

Restriction Enzyme Information for EZ011

Table 1. Restriction Enzymes That Do Not Cleave EZ011

Acc65IAfeIAflIIAgeIApaIAscIBaeIBaeI
BsiWIBstBIBstEIIBsu36IClaIEcoNIEcoO109IEcoRV
FseIHpaIKpnIPacIPmeIPmlIPpuMIPshAI
PspOMISacIISanDISbfISfiISgfISgrAISwaI
TliIXcmIXhoI


Table 2. Restriction Enzymes That Cleave EZ011 Once
AccB7I6964AccIII6728AdeI1470AhdI4449AhlI5621AjiI7637AloI1538Aor13HI6728
AspA2I1993AspEI4449AspI2321AsuNHI22AvrII1993BasI6964BbsI1156BbvCI7457
BcuI5621BlnI1993BlpI646BmeRI4449BmgBI7637BmtI26BpiI1156Bpu1102I646
BpuAI1156BpvUI4819BseAI6728BsmBI6956Bsp13I6728Bsp1720I646BspEI6728BspOI26
BstSNI5962BstV2I1156BstXI7720BtrI7637CciNI6412CelII646CpoI2719CsiI1760
CspCI5998CspI2719DinI2204DraIII1470DriI4449Eam1105I4449Eco105I5962Eco147I1992
EcoRI6732EgeI2204EheI2204Esp3I6956FauNDI5856HindIII6274KasI2202Kpn2I6728
MabI1760MluI5600Mly113I2203MroI6728MvrI4819NarI2203NdeI5856NheI22
NotI6412PasI6964PceI1992PciI3556PflFI2321PflMI6964Ple19I4819PluTI2206
PscI3556PsyI2321PvuI4819Rsr2I2719RsrII2719SexAI1760SfoI2204SgrDI5370
SnaBI5962SpeI5621SseBI1992SspDI2202StuI1992Tth111I2321Van91I6964XbaI13
XmaJI1993


Table 3. Restriction Enzymes That Cleave EZ011 Twice
AanI1595AanI3064Acc16I2305Acc16I4671AflIII3556AflIII5600Ama87I2014Ama87I7601
ArsI319ArsI6999Asp700I1665Asp700I5048AssI4929AssI7149AvaI2014AvaI7601
AviII2305AviII4671BamHI6482BamHI7185BcgI115BcgI4954BglII5384BglII7179
BmcAI4929BmcAI7149BmeT110I2014BmeT110I7601BsaXI1536BsaXI3431BsePI2600BsePI7226
BseRI55BseRI1989BseX3I2109BseX3I6412BsiHKCI2014BsiHKCI7601BsoBI2014BsoBI7601
Bsp68I2887Bsp68I5580BspHI4276BspHI5284BssHII2600BssHII7226BssNAI3177BssNAI7267
Bst1107I3177Bst1107I7267BstAPI1738BstAPI1810BstZ17I3177BstZ17I7267BstZI2109BstZI6412
BtuMI2887BtuMI5580CciI4276CciI5284Cfr9I2014Cfr9I7601EagI2109EagI6412
Ecl136II927Ecl136II6188EclXI2109EclXI6412Eco52I2109Eco52I6412Eco53kI927Eco53kI6188
Eco88I2014Eco88I7601EcoICRI927EcoICRI6188EcoT22I1743EcoT22I1815FspI2305FspI4671
MfeI782MfeI5533Mph1103I1743Mph1103I1815MroXI1665MroXI5048MunI782MunI5533
NruI2887NruI5580NsbI2305NsbI4671NsiI1743NsiI1815PagI4276PagI5284
PauI2600PauI7226PdmI1665PdmI5048PfoI305PfoI2980PsiI1595PsiI3064
Psp124BI929Psp124BI6190PstI2256PstI7858PteI2600PteI7226RcaI4276RcaI5284
RruI2887RruI5580SacI929SacI6190ScaI4929ScaI7149SmaI2016SmaI7603
SspI5253SspI7044SstI929SstI6190TspMI2014TspMI7601XmaI2014XmaI7601
XmnI1665XmnI5048ZrmI4929ZrmI7149Zsp2I1743Zsp2I1815

Table 4. Restriction Enzymes That Cleave EZ011 Three Times
AclI4675AclI5048AclI6819AleI55AleI205AleI382AlwNI3972AlwNI5452
AlwNI7511BclI954BclI2044BclI6279BsaAI1467BsaAI2507BsaAI5962BsaBI2062
BsaBI7184BsaBI7616BsaI4510BsaI6248BsaI7741Bse8I2062Bse8I7184Bse8I7616
BseJI2062BseJI7184BseJI7616BsgI153BsgI250BsgI574BsmI3125BsmI6725
BsmI7132Bso31I4510Bso31I6248Bso31I7741BspQI2547BspQI2757BspQI3440BstBAI1467
BstBAI2507BstBAI5962CaiI3972CaiI5452CaiI7511Eco31I4510Eco31I6248Eco31I7741
FbaI954FbaI2044FbaI6279KroI1362KroI2703KroI2988Ksp22I954Ksp22I2044
Ksp22I6279LguI2547LguI2757LguI3440MroNI1362MroNI2703MroNI2988Mva1269I3125
Mva1269I6725Mva1269I7132NaeI1364NaeI2705NaeI2990NgoMIV1362NgoMIV2703NgoMIV2988
OliI55OliI205OliI382PciSI2547PciSI2757PciSI3440PctI3125PctI6725
PctI7132PdiI1364PdiI2705PdiI2990Ppu21I1467Ppu21I2507Ppu21I5962Psp1406I4675
Psp1406I5048Psp1406I6819PstNI3972PstNI5452PstNI7511SapI2547SapI2757SapI3440