Vector Datasheet

ZX002 Datasheet

Vector Information

Catalog No.: ZX002

Description: Vector clone.

Whole Plasmid Size: 6300 bp
Vector: pEZX-FR02
Antibiotic: Ampicillin	Stable Selection Marker: N/A
Suggested Sequencing Primers: Forward: 5'-GATCCGCGAGATCCTGAT-3' Reverse: 5'-CCTATTGGCGTTACTATG-3'

Vector Information for ZX002

Restriction Enzyme Information for ZX002

Table 1. Restriction Enzymes That Do Not Cleave ZX002

AflIII

AgeI

AscI

BaeI

BbvCI

BlpI

Bpu10I

BssHII

BstBI

BstEII

BstZ17I

MluI

NotI

PacI

PciI

PflMI

PmlI

PpuMI

PshAI

RsrII

SacII

SalI

SanDI

SapI

SbfI

SgrAI

SmaI

SrfI

SwaI

XcmI

XmaI

Table 2. Restriction Enzymes That Cleave ZX002 Once


AccI	1235	AccIII	3190	AdeI	3407	AfeI	1622	AhdI	5340	AhlI	2347	AleI	1133	AloI	1465
AlwNI	5819	Aor13HI	3190	Aor51HI	1622	ApaI	665	AseI	5165	AsiSI	2314	AspA2I	343	AspEI	5340
AspI	3915	AsuNHI	623	AvrII	343	BarI	3238	BcuI	2347	BlnI	343	BmeRI	5340	BmtI	627
BseAI	3190	BsiWI	2324	BsmBI	3128	Bsp120I	661	Bsp13I	3190	Bsp1407I	1125	BspEI	3190	BspOI	627
BsrGI	1125	BstAUI	1125	BstSNI	2879	BstXI	1753	CaiI	5819	CspCI	2915	DraIII	3407	DriI	5340
Eam1105I	5340	Ecl136II	3105	Eco105I	2879	Eco147I	342	Eco32I	3704	Eco47III	1622	Eco53kI	3105	EcoICRI	3105
EcoRI	2318	EcoRV	3704	Esp3I	3128	FauNDI	2773	FblI	1235	HindIII	359	Kpn2I	3190	MroI	3190
MssI	2333	NdeI	2773	NheI	623	OliI	1133	PaeR7I	2341	PceI	342	Pfl23II	2324	PflFI	3915
PfoI	3499	PmeI	2333	PshBI	5165	Psp124BI	3107	PspLI	2324	PspOMI	661	PstNI	5819	PsyI	3915
RgaI	2314	SacI	3107	SfaAI	2314	SfiI	296	Sfr274I	2341	SgfI	2314	SlaI	2341	SnaBI	2879
SpeI	2347	SseBI	342	SspI	4535	SstI	3107	StrI	2341	StuI	342	Tth111I	3915	VspI	5165
XhoI	2341	XmaJI	343	XmiI	1235

Table 3. Restriction Enzymes That Cleave ZX002 Twice


AanI	2468	AanI	4295	AasI	380	AasI	6126	Acc36I	415	Acc36I	1566	Acc65I	3213	Acc65I	6293
AccBSI	3478	AccBSI	4498	AclI	4738	AclI	5111	AflII	391	AflII	588	AjiI	3404	AjiI	3662
Alw44I	4668	Alw44I	5914	Ama87I	2341	Ama87I	3240	ApaLI	4668	ApaLI	5914	ArsI	524	ArsI	1295
Asp700I	3753	Asp700I	4740	Asp718I	3213	Asp718I	6293	AvaI	2341	AvaI	3240	BalI	1616	BalI	2141
BamHI	2590	BamHI	4417	BbsI	499	BbsI	3173	BciVI	4503	BciVI	6030	BfrI	391	BfrI	588
BfuAI	415	BfuAI	1566	BfuI	4503	BfuI	6030	BglII	3205	BglII	6240	BmeT110I	2341	BmeT110I	3240
BmgBI	3404	BmgBI	3662	BpiI	499	BpiI	3173	BpuAI	499	BpuAI	3173	BpvUI	2314	BpvUI	4971
Bsa29I	2583	Bsa29I	4410	BsaAI	1837	BsaAI	2879	BseCI	2583	BseCI	4410	BseX3I	4168	BseX3I	4172
BshVI	2583	BshVI	4410	BsiHKCI	2341	BsiHKCI	3240	BsoBI	2341	BsoBI	3240	Bsp68I	2316	Bsp68I	3880
BspDI	2583	BspDI	4410	BspMI	415	BspMI	1566	BspTI	391	BspTI	588	BsrBI	3478	BsrBI	4498
BstAFI	391	BstAFI	588	BstBAI	1837	BstBAI	2879	BstV2I	499	BstV2I	3173	BstZI	4168	BstZI	4172
Bsu15I	2583	Bsu15I	4410	BsuI	4503	BsuI	6030	BtgZI	1253	BtgZI	2873	BtrI	3404	BtrI	3662
BtuMI	2316	BtuMI	3880	BveI	415	BveI	1566	ClaI	2583	ClaI	4410	CsiI	110	CsiI	1974
DrdI	380	DrdI	6126	DseDI	380	DseDI	6126	EagI	4168	EagI	4172	EclXI	4168	EclXI	4172
Eco52I	4168	Eco52I	4172	Eco88I	2341	Eco88I	3240	FalI	6268	FalI	6300	FseI	2046	FseI	4174
HincII	2488	HincII	4315	HindII	2488	HindII	4315	HpaI	2488	HpaI	4315	KpnI	3217	KpnI	6297
KspAI	2488	KspAI	4315	MabI	110	MabI	1974	MbiI	3478	MbiI	4498	MfeI	2497	MfeI	4324
MlsI	1616	MlsI	2141	MluNI	1616	MluNI	2141	MroXI	3753	MroXI	4740	MscI	1616	MscI	2141
Msp20I	1616	Msp20I	2141	MspCI	391	MspCI	588	MunI	2497	MunI	4324	MvrI	2314	MvrI	4971
NruI	2316	NruI	3880	PaeI	91	PaeI	163	PdmI	3753	PdmI	4740	PfeI	365	PfeI	3330
Ple19I	2314	Ple19I	4971	Ppu21I	1837	Ppu21I	2879	PsiI	2468	PsiI	4295	Psp1406I	4738	Psp1406I	5111
PvuI	2314	PvuI	4971	PvuII	19	PvuII	709	RigI	2046	RigI	4174	RruI	2316	RruI	3880
SexAI	110	SexAI	1974	SphI	91	SphI	163	TfiI	365	TfiI	3330	Vha464I	391	Vha464I	588
VneI	4668	VneI	5914	XbaI	2292	XbaI	4155	XmnI	3753	XmnI	4740

Table 4. Restriction Enzymes That Cleave ZX002 Three Times


Acc16I	1457	Acc16I	5117	Acc16I	6247	AssI	601	AssI	800	AssI	4859	AviII	1457	AviII	5117
AviII	6247	AxyI	1248	AxyI	1848	AxyI	3805	BauI	1562	BauI	4671	BauI	6055	BmcAI	601
BmcAI	800	BmcAI	4859	BsaI	453	BsaI	3759	BsaI	5274	BsaXI	951	BsaXI	1463	BsaXI	3366
Bse21I	1248	Bse21I	1848	Bse21I	3805	Bso31I	453	Bso31I	3759	Bso31I	5274	BspHI	1218	BspHI	4500
BspHI	5508	BssSI	1562	BssSI	4671	BssSI	6055	Bst2BI	1562	Bst2BI	4671	Bst2BI	6055	BstAPI	88
BstAPI	160	BstAPI	3407	BstENI	824	BstENI	1247	BstENI	2252	Bsu36I	1248	Bsu36I	1848	Bsu36I	3805
CciI	1218	CciI	4500	CciI	5508	Eco31I	453	Eco31I	3759	Eco31I	5274	Eco81I	1248	Eco81I	1848
Eco81I	3805	EcoNI	824	EcoNI	1247	EcoNI	2252	EcoT22I	93	EcoT22I	165	EcoT22I	3438	FspI	1457
FspI	5117	FspI	6247	KroI	2042	KroI	2063	KroI	4170	Mph1103I	93	Mph1103I	165	Mph1103I	3438
MroNI	2042	MroNI	2063	MroNI	4170	NaeI	2044	NaeI	2065	NaeI	4172	NgoMIV	2042	NgoMIV	2063
NgoMIV	4170	NsbI	1457	NsbI	5117	NsbI	6247	NsiI	93	NsiI	165	NsiI	3438	PagI	1218
PagI	4500	PagI	5508	PasI	1390	PasI	1777	PasI	3993	PdiI	2044	PdiI	2065	PdiI	4172
RcaI	1218	RcaI	4500	RcaI	5508	ScaI	601	ScaI	800	ScaI	4859	TspGWI	2312	TspGWI	4559
TspGWI	4901	XagI	824	XagI	1247	XagI	2252	ZrmI	601	ZrmI	800	ZrmI	4859	Zsp2I	93
Zsp2I	165	Zsp2I	3438