miExpress™ miRNA前体表达克隆

产品介绍

MicroRNA ( miRNA )是真核细胞转录形成的非编码单链小分子RNA。它们的序列高度保守,通常是21到23个核苷酸长度,几乎与所有的细胞功能和基因表达调控有关。它们通过靶定到mRNA 3'非翻译区(3' UTR)来调节基因的表达。MicroRNA靶标基因的表达失调与癌症、 心血管疾病等各种疾病的发生有关。

GeneCopoeia提供eGFP作为报告基因的miExpress™ miRNA前体表达克隆,将miRNA的茎环前体(约150核苷酸)克隆到病毒和非病毒载体可以感染几乎所有细胞。

优势        

覆盖面广

  • 涵盖了miRBase最新数据库中几乎所有已知人、小鼠和大鼠miRNA

灵活的载体选择

  • 慢病毒载体可转导普通细胞和难转染、未分化细胞
  • 非病毒载体可进行稳定或瞬时转染

优化的表达框

  • miRNA表达框已完全测序确证并经过优化,有利于前体miRNA在细胞中的表达和成熟

可选标记和报告基因

  • 载体中包含的eGFP标签可以监测转染或转导效率
  • 新霉素和嘌呤霉素标记可用于稳定细胞株的筛选

图1. miRNA前体表达克隆和miRNA 3' UTR克隆在miRNA调控过程中的作用机理

 

载体

miRNA前体表达克隆载体

 
 
载体
示踪基因
筛选标记
启动子
病毒类型
pEZX-MR03 eGFP Puromycin CMV HIV
pEZX-MR04 eGFP Puromycin CMV Mammalian
 

 

 

 

 

慢病毒载体的miRNA前体表达克隆

GeneCopoeia提供客户定制的慢病毒载体miRNA前体表达克隆,周期为2-5周。

 

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克隆套装

miRNA前体表达克隆套装

GeneCopoeia除了提供单个克隆,还提供预制和定制的miRNA前体表达克隆套装,了解详情,请发邮件到support@igenebio.com或拨打4006-020-200。

货号 产品描述 miRNA 载体 价格
MPC-HG-MR03 Human whole genome miRNA  precursor clone set (lentiviral) 909

pEZX-MR03 Lentiviral

Inquire
Various Human cancer miRNA precursor clone sets (lentiviral) Various
View all cancer sets below

pEZX-MR03 Lentiviral

Inquire
MPC-HG-MR04 Human whole genome miRNA  precursor clone set (non-viral) 671

pEZX-MR04 Non-viral

Inquire
Various Human cancer miRNA precursor clone sets (non-viral) Various
View all cancer sets below

pEZX-MR04 Non-viral

Inquire
MPC-MG-MR03 Mouse genome miRNA precursor clone set (lentiviral) 327

pEZX-MR03 Lentiviral

Inquire
MPC-MG-MR04 Mouse genome miRNA precursor clone set (non-viral) 519 pEZX-MR04 Non-viral Inquire
MPC-RG-MR03 Rat genome miRNA precursor clone set (lentiviral) 163

pEZX-MR03 Lentiviral

Inquire
MPC-RG-MR04 Rat genome miRNA precursor clone set (non-viral) 309 pEZX-MR04 Non-viral Inquire

 

人癌症miRNA前体表达克隆套装(慢病毒载体)

货号 产品描述 癌症 miRNA 价格
MPC-HCY-MR03  Human Bladder Cancer miRNA Precursor Clone Set (lentiviral) Bladder Cancer 56 Inquire
MPC-HCN-MR03  Human Brain Cancer miRNA Precursor Clone Set (lentiviral) Brain Cancer 178 Inquire
MPC-HCB-MR03  Human Breast Cancer miRNA Precursor Clone Set (lentiviral) Breast Cancer 150 Inquire
MPC-HCV-MR03  Human Cervical Cancer miRNA Precursor Clone Set (lentiviral) Cervical Cancer 21 Inquire
MPC-HCI-MR03  Human Cholangiocarcinoma miRNA Precursor Clone Set (lentiviral) Cholangiocarcinoma 28 Inquire
MPC-HCC-MR03  Human Colorectal Cancer miRNA Precursor Clone Set (lentiviral) Colorectal Cancer 145 Inquire
MPC-HCW-MR03  Human Endometrial Cancer miRNA Precursor Clone Set (lentiviral) Endometrial Cancer 72 Inquire
MPC-HCG-MR03  Human Gastric Cancer miRNA Precursor Clone Set (lentiviral) Gastric Cancer 61 Inquire
MPC-HCT-MR03  Human Head and Neck Cancer miRNA Precursor Clone Set (lentiviral) Head and Neck Cancer 144 Inquire
MPC-HCH-MR03  Human Hepatocellular Carcinoma miRNA Precursor Clone Set (lentiviral) Hepatocellular Carcinoma 134 Inquire
MPC-HCK-MR03  Human Kidney Cancer miRNA Precursor Clone Set (lentiviral) Kidney Cancer 64 Inquire
MPC-HCX-MR03  Human Leukemia miRNA Precursor Clone Set (lentiviral) Leukemia 131 Inquire
MPC-HCL-MR03  Human Lung Cancer miRNA Precursor Clone Set (lentiviral) Lung Cancer 136 Inquire
MPC-HCZ-MR03  Human Lymphoma miRNA Precursor Clone Set (lentiviral) Lymphoma 85 Inquire
MPC-HCM-MR03  Human Melanoma miRNA Precursor Clone Set (lentiviral) Melanoma 106 Inquire
MPC-HCE-MR03  Human Mesothelioma miRNA Precursor Clone Set (lentiviral) Mesothelioma 21 Inquire
MPC-HCR-MR03  Human Myeloma miRNA Precursor Clone Set (lentiviral) Myeloma 39 Inquire
MPC-HCO-MR03  Human Ovarian Cancer miRNA Precursor Clone Set (lentiviral) Ovarian Cancer 141 Inquire
MPC-HCP-MR03  Human Pancreatic Cancer miRNA Precursor Clone Set (lentiviral) Pancreatic Cancer 156 Inquire
MPC-HCA-MR03  Human Pituitary Adenoma miRNA Precursor Clone Set (lentiviral) Pituitary Adenoma 28 Inquire
MPC-HCQ-MR03  Human Prostate Cancer miRNA Precursor Clone Set (lentiviral) Prostate Cancer 122 Inquire
MPC-HCS-MR03  Human Sarcoma miRNA Precursor Clone Set (lentiviral) Sarcoma 22 Inquire
MPC-HCJ-MR03  Human Thyroid Carcinoma miRNA Precursor Clone Set (lentiviral) Thyroid Carcinoma 41 Inquire
MPC-HCU-MR03  Human Uterine Leiomyoma miRNA Precursor Clone Set (lentiviral) Uterine Leiomyoma 30 Inquire

 

人癌症miRNA前体表达克隆套装(非病毒载体)

货号 产品描述 癌症 miRNA 价格
MPC-HCY-MR04  Human Bladder Cancer Precursor miRNA Clone Set (non-viral) Bladder Cancer 56 Inquire
MPC-HCN-MR04  Human Brain Cancer Precursor miRNA Clone Set (non-viral) Brain Cancer 173 Inquire
MPC-HCB-MR04  Human Breast Cancer Precursor miRNA Clone Set (non-viral) Breast Cancer 143 Inquire
MPC-HCV-MR04  Human Cervical Cancer Precursor miRNA Clone Set (non-viral) Cervical Cancer 20 Inquire
MPC-HCI-MR04  Human Cholangiocarcinoma Precursor miRNA Clone Set (non-viral) Cholangiocarcinoma 26 Inquire
MPC-HCC-MR04  Human Colorectal Cancer Precursor miRNA Clone Set (non-viral) Colorectal Cancer 140 Inquire
MPC-HCW-MR04  Human Endometrial Cancer Precursor miRNA Clone Set (non-viral) Endometrial Cancer 72 Inquire
MPC-HCG-MR04  Human Gastric Cancer Precursor miRNA Clone Set (non-viral) Gastric Cancer 59 Inquire
MPC-HCT-MR04  Human Head and Neck Cancer Precursor miRNA Clone Set (non-viral) Head and Neck Cancer 140 Inquire
MPC-HCH-MR04  Human Hepatocellular Carcinoma Precursor miRNA Clone Set (non-viral) Hepatocellular Carcinoma 129 Inquire
MPC-HCK-MR04  Human Kidney Cancer Precursor miRNA Clone Set (non-viral) Kidney Cancer 61 Inquire
MPC-HCX-MR04  Human Leukemia Precursor miRNA Clone Set (non-viral) Leukemia 125 Inquire
MPC-HCL-MR04  Human Lung Cancer Precursor miRNA Clone Set (non-viral) Lung Cancer 133 Inquire
MPC-HCZ-MR04  Human Lymphoma Precursor miRNA Clone Set (non-viral) Lymphoma 80 Inquire
MPC-HCM-MR04  Human Melanoma Precursor miRNA Clone Set (non-viral) Melanoma 102 Inquire
MPC-HCE-MR04  Human Mesothelioma Precursor miRNA Clone Set (non-viral) Mesothelioma 20 Inquire
MPC-HCR-MR04  Human Myeloma Precursor miRNA Clone Set (non-viral) Myeloma 37 Inquire
MPC-HCO-MR04  Human Ovarian Cancer Precursor miRNA Clone Set (non-viral) Ovarian Cancer 136 Inquire
MPC-HCP-MR04  Human Pancreatic Cancer Precursor miRNA Clone Set (non-viral) Pancreatic Cancer 154 Inquire
MPC-HCA-MR04  Human Pituitary Adenoma Precursor miRNA Clone Set (non-viral) Pituitary Adenoma 27 Inquire
MPC-HCQ-MR04  Human Prostate Cancer Precursor miRNA Clone Set (non-viral) Prostate Cancer 120 Inquire
MPC-HCS-MR04  Human Sarcoma Precursor miRNA Clone Set (non-viral) Sarcoma 22 Inquire
MPC-HCJ-MR04  Human Thyroid Carcinoma Precursor miRNA Clone Set (non-viral) Thyroid Carcinoma 40 Inquire
MPC-HCU-MR04  Human Uterine Leiomyoma Precursor miRNA Clone Set (non-viral) Uterine Leiomyoma 30 Inquire

 

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