基于HIV骨架的慢病毒颗粒包装试剂 (20 reactions)
基于HIV骨架的慢病毒颗粒包装试剂 (40 reactions)
基于HIV骨架的慢病毒颗粒包装试剂 (5 reactions)
基于qRT-PCR技术的HIV骨架慢病毒颗粒滴度检测试剂 （20次RT反应 +50次PCR反应）
基于qRT-PCR技术的HIV骨架慢病毒颗粒滴度检测试剂 （40次RT反应 +100次PCR反应）
慢病毒浓缩试剂盒（50 ml）
293Ta慢病毒颗粒包装细胞系 (1.5 x 106 cells)
产品介绍
慢病毒包装试剂盒
GeneCopoeia的Lenti-Pac™ HIV 表达包装系统包括优化的慢病毒包装质粒，eGFP阳性对照质粒，EndoFectin™转染试剂，增加病毒产量的TiterBoost™滴度增强剂。该系统与GeneCopoeia的ORF、shRNA、miRNA及Promoter克隆配套使用保证更高滴度和更高表达水平。
HIV包装系统中的包装质粒经过设计调整，确保将重组子包装成具有高感染率的慢病毒颗粒，同时防止了病毒的自我复制。
293Ta慢病毒包装细胞系
293Ta包装细胞系是常用于病毒包装的工具细胞。Lenti-Pac™ 293Ta包装细胞系转染效率高，能够实现病毒蛋白高水平的表达。
慢病毒浓缩试剂
Lenti-Pac™ 慢病毒浓缩试剂是GeneCopoeia为用户设计的用于快速、简便浓缩慢病毒颗粒的慢病毒浓缩试剂。实验操作时只需将浓缩试剂与需浓缩的慢病毒上清液混合，经短暂孵育，普通离心机离心即可。
慢病毒滴度检测试剂盒
GeneCopoeia设计的Lenti-Pac™ HIV qRT-PCR慢病毒滴度检测试剂盒可快速、简便地测定病毒拷贝数，并确定病毒包装是否成功。该试剂盒主要组分包括RNA抽提试剂，qRT-PCR反应试剂以及内参对照。慢病毒的RNA基因组被反转录后用基于SYBR® Green I原理的qRT-PCR技术进行定量。
相关产品
- 慢病毒载体的克隆 — ORF、shRNA、miRNA、miRNA抑制剂以及Promoter克隆
- 预制慢病毒颗粒 — iPSC基因、ORF克隆、阳性和阴性对照等
- 特殊慢病毒包装服务 — 价格低且周期短，包含ORF，miRNA或miRNA inhibitors序列的慢病毒载体
- 客户定制的慢病毒服务 — 40多种载体和多种融合标签，可定制任意ORF，shRNA或miRNA载体
2023已发表的文章
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- Yu, C., et al. (2023). Flot2 acts as a novel mediator of podocyte injury in proteinuric kidney disease. Int J Biol Sci doi: 10.7150/ijbs.78945 [OMICSLINK™ empty vector; GLuc-ON™ promoter reporter clone; Lenti-Pac™ HIV Expression Packing Kit; Secrete-Pair™ Dual Luminescence Assay Kit]
- Li, X., et al. (2023). Ultrasensitive sensors reveal the spatiotemporal landscape of lactate metabolism in physiology and disease. Cell Metabolism doi: 10.1016/j.cmet.2022.10.002 [Lenti-Pac™ lentivirus concentration kit]
- Yang, Q., et al. (2023). N4‐Acetylcytidine Drives Glycolysis Addiction in Gastric Cancer via NAT10/SEPT9/HIF‐1α Positive Feedback Loop. Advanced Science doi: 10.1002/advs.202300898 [Lenti-Pac™ HIV Expression Packaging Kit; OMICSLINK™ lentiviral shRNA clone; miTarget™ 3′ UTR Clone; Luc-Pair™ Duo-Luciferase HS Assay Kit]
- Wong, S.W.K., et al. (2023). Small Extracellular Vesicle-Derived vWF Induces a Positive Feedback Loop between Tumor and Endothelial Cells to Promote Angiogenesis and Metastasis in Hepatocellular Carcinoma. Advanced Science doi: 10.1002/advs.202302677 [Lenti-Pac™ HIV Expression Packaging Kit]
- Li, L., et al. (2023). FSCN1 promotes proliferation; invasion and glycolysis via the IRF4/AKT signaling pathway in oral squamous cell carcinoma. BMC Oral Health doi: 10.1186/s12903-023-03191-9 [OMICSLINK™ FSCN1 shRNA clone; Lenti-Pac™ HIV expression packaging kit]
- Passariello, M., et al. (2023). Comparative Analysis of a Human Neutralizing mAb Specific for SARS-CoV-2 Spike-RBD with Cilgavimab and Tixagevimab for the Efficacy on the Omicron Variant in Neutralizing and Detection Assays. Int J Mol Sci doi: 10.3390/ijms241210053 [Lenti-Pac™ SARS-CoV-2 Truncated Spike protein-pseudotyped (D614G) Lentivirus Packaging Kit]
- Tien, J., et al. (2023). Modifying antibody-FcRn interactions to increase the transport of antibodies through the blood-brain barrier. MAbs doi: 10.1080/19420862.2023.2229098 [OMICSLINK™ mouse Fcgrt, B2m Lentiviral ORF expression clone]
- Cruz, M.A., et al. (2023). Micronutrient optimization for tissue engineered articular cartilage production of type II collagen. Front Bioeng Biotechnol doi: 10.3389/fbioe.2023.1179332 [Lenti-Pac™ HIV Expression Packaging Kit; GLuc-ON™ COL2A1 promoter-reporter clone]
- Guo, L., et al. (2023). Directed natural evolution generates a next-generation oncolytic virus with a high potency and safety profile. Nat Commun doi: 10.1038/s41467-023-39156-3 [OMICSLINK™ nsP3 Lentiviral ORF expression clone]
- Li, K., et al. (2023). Dysregulation of PLOD2 Promotes Tumor Metastasis and Invasion in Hepatocellular Carcinoma. J Clin Transl Hepatol doi: 10.14218/JCTH.2022.00401 [Lenti-Pac™ HIV Expression Packaging Kit; EndoFectin™ Lenti]
- Hosaka, K., et al. (2023). Perivascular localized cells commit erythropoiesis in PDGF‐B‐expressing solid tumors. Cancer Commun (Lond) doi: 10.1002/cac2.12423 [Lenti-Pac™ HIV Expression Packaging Kit]
- Tsukui, D., et al. (2023). GM-CSF receptor/SYK/JNK/FOXO1/CD11c signaling promotes atherosclerosis. iScience doi: 10.1016/j.isci.2023.107293 [OMICSLINK™ Lentiviral expression clone; Secrete-Pair™ Dual Luminescence Assay Kit]
- Yuan, S-S.F., et al. (2023). IL-1RA promotes oral squamous cell carcinoma malignancy through mitochondrial metabolism-mediated EGFR/JNK/SOX2 pathway. J Transl Med doi: 10.1186/s12967-023-04343-9 [OMICSLINK™ IL1RN Lentiviral ORF expression clone]
- Muoio, M.G., et al. (2023). RAGE inhibition blunts insulin-induced oncogenic signals in breast cancer. Breast Cancer Res doi: 10.1186/s13058-023-01686-5 [Lenti-Pac™ 293Ta cell line]
- Yoshizue, T., et al. (2023). Antisera Produced Using an E. coli-Expressed SARS-CoV-2 RBD and Complemented with a Minimal Dose of Mammalian-Cell-Expressed S1 Subunit of the Spike Protein Exhibits Improved Neutralization. Int J Mol Sci doi: 10.3390/ijms241310583[Lenti-Pac™ SARS-CoV-2 Truncated Spike protein-pseudotyped (D614G) Lentivirus Packaging Kit]
- Wang, X-X., et al. (2023). CCNB1 is involved in bladder cancer pathogenesis and silencing CCNB1 decelerates tumor growth and improves prognosis of bladder cancer. Exp Ther Med doi: 10.3892/etm.2023.12081 [Lenti-Pac™ HIV Expression Packaging Kit]
- Li, F., et al. (2023). RIPK1-dependent necroptosis promotes vasculogenic mimicry formation via eIF4E in triple-negative breast cancer. Cell Death Dis doi: 10.1038/s41419-023-05841-w [Lenti-Pac™ HIV Expression Packaging Kit; OMICSLINK™ RIPK1, EIF4E ORF expression clone; OMICSLINK™ RIPK1 shRNA expression clones]
- Wei, Y., et al. (2023). LncRNA XIST promotes adjuvant-induced arthritis by increasing the expression of YY1 via miR-34a-5p. Arch Rheumatol doi: 10.46497/ArchRheumatol.2022.9250 [miExpress™ inhibitor clone; OMICSLINK™ lentiviral shRNA clone; OMICSLINK™ ORF expression clones; Lenti-Pac™ HIV Expression Packaging Kit]
- Huang, X., et al. (2023). Downregulation of LncRNA GCLC-1 Promotes Microcystin-LR-Induced Malignant Transformation of Human Liver Cells by Regulating GCLC Expression. Toxics doi: 10.3390/toxics11020162 [OMICSLINK™ shRNA clones targeting LOC101927136; Lenti-Pac™ HIV Expression Packaging Kit]
- Mansouri, M., et al. (2023). Protein Networks Associated with Native Metabotropic Glutamate 1 Receptors (mGlu1) in the Mouse Cerebellum. Cells doi: 10.3390/cells12091325 [OMICSLINK™ mouse Glu1α ORF expression clone; OMICSLINK™ mouse KCTD12 lentiviral ORF expression clone]
- Parween, F., et al. (2023). Chemokine positioning determines mutually exclusive roles for their receptors in extravasation of pathogenic human T cells. bioRxiv doi: 10.1101/2023.01.25.525561 [Lentifect™ lentiviral particles]
- Singh, S.P., et al. (2023). Human CCR6+ Th cells show both an extended stable gradient of Th17 activity and imprinted plasticity. bioRxiv doi: 10.1101/2023.01.05.522630 [Lentifect™ lentiviral particles; OMICSLINK™ Lentiviral ORF expression clone]
- Kim, G.S., et al. (2023). Single-cell analysis identifies Ifi27l2a as a novel gene regulator of microglial inflammation in the context of aging and stroke. Res Sq doi: 10.21203/rs.3.rs-2557290/v1 [Lentifect™ lentiviral particles]
- Zhu, Y., et al. (2023). Research on Radiosensitivity of the Protein Kinase B Signaling Pathway in Cervical Cancer. Comput Math Methods Med doi: 10.1155/2021/6350038 [Lenti-Pac™ HIV Expression Packaging Kit; OMICSLINK™ shRNA clone]
- Liu, Q., et al. (2023). A long non-coding RNA H19/microRNA-138/TLR3 network is involved in high phosphorus-mediated vascular calcification and chronic kidney disease. Cell Cycle doi: 10.1080/15384101.2022.2064957 [Lentifect™ lentiviral particles]
- Li, X., et al. (2023). Beclin1- and Atg13-dependent autophagy activation and morroniside have synergistic effect on osteoblastogenesis. Exp Biol Med (Maywood) doi: 10.1177/15353702221116879 [Lenti-Pac™ HIV Expression Packaging Kit; OMICSLINK™ Lentiviral ORF expression clone]
- Han, W., et al. (2023). Exploiting the tumor-suppressive activity of the androgen receptor by CDK4/6 inhibition in castration-resistant prostate cancer. Mol Ther doi: 10.1016/j.ymthe.2022.01.039 [Lentifect™ shRNA lentiviral particles]
- Hasan, K.M., et al. (2023). Fatty Acid Excess Dysregulates CARF to Initiate the Development of Hepatic Steatosis. Cells doi: 10.3390/cells12071069 [AAVPrime™ AAV particles expressing CARF and AAV-empty vector; OMICSLINK™ CARF Lentiviral ORF expression clone]
- Zhang, X., et al. (2023). IGFBP3 induced by the TGF-β/EGFRvIII transactivation contributes to the malignant phenotype of glioblastoma. iScience doi: 10.1016/j.isci.2023.106639 [shIGFBP3 lentiviral particles and control (psi-LvRU6rLP) ; Lentifect™ shRNA lentiviral particles]
- Lang, L., et al. (2023). Adaptive c-Met-PLXDC2 Signaling Axis Mediates Cancer Stem Cell Plasticity to Confer Radioresistance-associated Aggressiveness in Head and Neck Cancer. Cancer Res Commun doi: 10.1158/2767-9764.CRC-22-0289 [Lenti-Pac™ 293Ta Cell Line]
- Georgakopoulos-Soares, L., et al. (2023). Transcription factor binding site orientation and order are major drivers of gene regulatory activity. Nat Commun doi: 10.1038/s41467-023-37960-5 [Lenti-Pac™ HIV Expression Packaging Kit]
- Huang, W-J., et al. (2023). The β-catenin-LINC00183-miR-371b-5p-Smad2/LEF1 axis promotes adult T-cell lymphoblastic lymphoma progression and chemoresistance. J Exp Clin Cancer Res doi: 10.1186/s13046-023-02670-9 [8 mg/mL polybrene]
- Shi, Y., et al. (2023). PRMT3-mediated arginine methylation of IGF2BP1 promotes oxaliplatin resistance in liver cancer. Nat Commun doi: 10.1038/s41467-023-37542-5 [dCas9 lentivirus]
- Jiang, L., et al. (2023). Chaperoning of specific tau structure by immunophilin FKBP12 regulates the neuronal resilience to extracellular stress. Sci Adv doi: 10.1126/sciadv.add9789 [OMICSLINK™ NEUROG2 Lentiviral ORF expression clone]
- Zhang, R., et al. (2023). Mesenchymal stem cell suppresses the efficacy of CAR-T toward killing lymphoma cells by modulating the microenvironment through stanniocalcin-1. eLife doi: 10.7554/eLife.82934 [Lenti-Pac™ HIV expression packaging kit]
- Deng,Y., et al. (2023). NUF2 Promotes Breast Cancer Development as a New Tumor Stem Cell Indicator. Int J Mol Sci doi: 10.3390/ijms24044226 [Lenti-Pac™ HIV qRT-PCR Titration Kit]
- Stierschneider, A., et al. (2023). Comparative and Temporal Characterization of LPS and Blue-Light-Induced TLR4 Signal Transduction and Gene Expression in Optogenetically Manipulated Endothelial Cells. Cells doi: 10.3390/cells12050697 [Lenti-Pac™ 293Ta Cell Line]
- Koesterich, J., et al. (2023). Characterization of De Novo Promoter Variants in Autism Spectrum Disorder with Massively Parallel Reporter Assays. Int J Mol Sci doi: 10.3390/ijms24043509 [Lenti-PAC™ HIV Expression Packaging Kit]
- Jia, Y., et al. (2023). Tripartite motif containing 69 elicits ERK2-dependent EYA4 turnover to impart pancreatic tumorigenesis. J Cancer doi: 10.7150/jca.79905 [TRIM69 sgRNA lentivirus]
- Luo, F., et al. (2023). Hypermethylation of HIC2 is a potential prognostic biomarker and tumor suppressor of glioma based on bioinformatics analysis and experiments. CNS Neurosci Ther doi: 10.1111/cns.14093 [Lenti-PAC™ HIV Expression Packaging Kit]
- Rao, X., et al. (2023). Dipeptidyl Peptidase 4/Midline‐1 Axis Promotes T Lymphocyte Motility in Atherosclerosis. Adv Sci (Weinh) doi: 10.1002/advs.202204194 [Lentifect™ Lentiviral particles expressing Mid1]
- Fuller, M.J., et al. (2023). Investigating role of ASIC2 in synaptic and behavioral responses to drugs of abuse. Front Mol Biosci doi: 10.3389/fmolb.2023.1118754 [Lentifect™ Lentiviral particles expressing Asic2a and Asic2b]
- Huang, Z., et al. (2023). CaMKII may regulate renal tubular epithelial cell apoptosis through YAP/NFAT2 in acute kidney injury mice. Ren Fail doi: 10.1080/0886022X.2023.2172961 [Lenti-Pac™ HIV Expression Packing Kit]
- Zhang, C., et al. (2023). Carbonic Anhydrase IX Controls Vulnerability to Ferroptosis in Gefitinib-Resistant Lung Cancer. Oxid Med Cell Longev doi: 10.1155/2023/1367938 [lentiviral vector]
- Zhang, Y., et al. (2023). Extracellular Vesicles Expressing CD19 Antigen Improve Expansion and Efficacy of CD19-Targeted CAR-T Cells. Int J Nanomedicine doi: 10.2147/IJN.S390720 [lentiviral vector pCDH-CMV-CD19]
- Pallichankandy, S., et al. (2023). Targeting oxeiptosis-mediated tumor suppression: a novel approach to treat colorectal cancers by sanguinarine. Cell Death Discov doi: 10.1038/s41420-023-01376-3 [Lenti-Pac™ Expression Packaging Kit]
- Chen, Y., et al. (2023). LncRNA TGFB2-OT1 Promotes Progression and Angiogenesis in Hepatocellular Carcinoma by Dephosphorylating β-Catenin. J Hepatocell Carcinoma doi: 10.2147/JHC.S404008 [empty control vector]
- Zhang, R., et al. (2023). Knockdown SENP1 Suppressed the Angiogenic Potential of Mesenchymal Stem Cells by Impacting CXCR4-Regulated MRTF-A SUMOylation and CCN1 Expression. Biomedicines doi: 10.3390/biomedicines11030914 [Lenti-Pac™ HIV Expression Packaging Kit]
- Ji, Q., et al. (2023). PYGL-mediated glucose metabolism reprogramming promotes EMT phenotype and metastasis of pancreatic cancer. Int J Biol Sci doi: 10.7150/ijbs.76756 [OMICSLINK™ PYGL lentiviral overexpression clone]
- Lu, J., et al. (2023). LZTFL1 inhibits kidney tumor cell growth by destabilizing AKT through ZNRF1-mediated ubiquitin proteosome pathway. Oncogene doi: 10.1038/s41388-023-02666-x [Lenti-PAC™ HIV Expression Packaging Kit; Lenti-Pac™ lentivirus concentration kit]
- Subramaniyan, B., et al. (2023). The Isolation and In Vitro Differentiation of Primary Fetal Baboon Tracheal Epithelial Cells for the Study of SARS-CoV-2 Host-Virus Interactions. Viruses doi: 10.3390/v15040862 [OMICSLINK™ ACE2 lentiviral ORF expression clone; empty vector control]
- Nieddu, V., et al. (2023). Matrix Gla Protein drives stemness and tumor initiation in ovarian cancer. Cell Death Dis doi: 10.1038/s41419-023-05760-w [OMICSLINK™ MGP ORF expression clone]
- Wang, S., et al. (2023). Myc derived circRNA promotes triple-negative breast cancer progression via reprogramming fatty acid metabolism. Discov Oncol doi: 10.1007/s12672-023-00679-2 [lentivirus vector psi-LVRU6GP]
- Hung, Y-W., et al. (2023). Extracellular arginine availability modulates eIF2α O-GlcNAcylation and heme oxygenase 1 translation for cellular homeostasis. J Biomed Sci doi: 10.1186/s12929-023-00924-4 [lentiviral vectors]
