GeneCopoeia 的Lentifect™ SARS-CoV-2 S 蛋白假慢病毒由特定优化的包装体系制备而成。以SARS-CoV-2 Spike糖蛋白替代慢病毒包装体系中的囊泡性口炎病毒G糖蛋白(VSV-G)为衣壳蛋白进行包装制备成“假病毒”,该病毒可有效感染含ACE2(SARS-CoV-2的人类受体)的细胞。(Ou, X., et al., 2020. Nature Communications doi: 10.1038/s41467-020-15562-9; Hoffman, M., et al., 2020. Cell doi: 10.1016/j.cell.2020.02.052)。
Lentifect™ SARS-CoV-2 S 蛋白假慢病毒应用:
- 预防SARS-CoV-2病毒感染的疫苗研制
- 抗SARS-CoV-2病毒中和抗体的作用和机制研究
- 开发抗病毒治疗药物
- 研究病毒-受体相互作用的机制
Lentifect™ SARS-CoV-2 S 蛋白假慢病毒优势:
- 以新冠病毒S蛋白作为表面衣壳糖蛋白
- S蛋白多样性,全长、截短、点突变可供选择
- 提供eGFP和luc蛋白标记,同时满足不同研究需求
- 不具备致病性,安全可靠,只需P2实验室环境
注意:SARS-CoV-2 S 蛋白假慢病毒仅用于研究目的。它不用于人类的诊断或治疗。
可从下方的 “Spike突变位点列表”了解突变位点信息。 GeneCopoeia使用GISAID数据库得到每个变异体中最常见的突变信息,从而设计我们的假病毒颗粒。这些突变分布见下表。 请注意,每个突变株都有不同的亚型,以至于可能与我们所提供的假病毒颗粒的突变位点略有不同。如果您想定制与我们目前提供的不同的突变株假病毒,请联系我们4006-020-200或sales@igenebio.com。 GeneCopoeia已生产出单独表达人ACE2或ACE2与TMPRSS2联合表达的HEK293T细胞系,TMPRSS2是一种促进Spike-ACE2受体相互作用的蛋白酶。这些细胞系高水平表达ACE2和/或TMPRRSS2 mRNA(图1)。由于细胞表面显示ACE2蛋白(图2)和/或TMPRSS2蛋白(图3),这些细胞易于被其包膜中表达SARS-CoV-2 S蛋白的病毒感染。 mCherry/ Puromycin, Hygromycin 欲了解更多信息或询价,请联系GeneCopoeia技术支持support@igenebio.com或拨打4006-020-200 Warranty statement and limited use license 购买须知 性能数据 GeneCopoeia的研发团队测试了SARS-CoV-2 S 蛋白假慢病毒感染ACE2表达细胞的能力,如下图4所示。 图4. 用表达eGFP/hLuc的SARS-CoV-2 S假慢病毒(假病毒)转导表达ACE2-或ACE2/TMPRSS2的HEK293T细胞。用10 μl假病毒在96孔板中转导每个细胞系。转导72小时后,在荧光显微镜下对细胞进行拍照,随后测量荧光素酶活性或进行FACS分选。SL221:表达ACE2的HEK293T细胞。SL222:表达ACE2和TMPRSS2的HEK293T细胞。SP101:全长S蛋白,D614变体。SP103:全长S蛋白,D614G变体。SP001:截短S蛋白,D614变体。SP003:截短S蛋白,D614G变体。A. 荧光显微镜。B. 荧光素酶活性测定。使用GeneCopoeia的Luc-Pair™ Firefly Luciferase HS Assay Kit(货号LF007),从假病毒转导细胞培养物中提取20 μl细胞裂解液,分析萤火虫荧光素酶活性,用FACS分选法测定转导的单位滴度units/ml。 相关产品及服务 在线研讨会 标题:来自GeneCopoeia的COVID-19冠状病毒研究的先进工具 2020年9月9日,星期三 由SARS-CoV-2冠状病毒引起的新冠肺炎全球大流行凸显了对诊断试剂、抗病毒治疗和疫苗开发的迫切需求。在本次网络研讨会中,我们将讨论GeneCopoeia先进的解决方案,包括检测试剂盒、SARS-CoV-2假病毒以及研究对抗这种致命疾病所需的其他工具。 观看录制的网络研讨会/下载幻灯片 已发表的文章 查看近期使用GeneCopoeia SARS-CoV-2 S蛋白假慢病毒产品的已发表文章 2022 2021 2020 SARS-CoV-2 截短S蛋白慢病毒(去除了19个氨基酸内质网保留信号,体外级别)
Spike突变位点列表
SARS-CoV-2 variant
Spike mutations
Wild type (catalog numbers SP001-100, SP101-100)
None
D614G (catalog numbers SP003-100, SP103-100)
D614G
N439K (catalog numbers SP004-100, SP104-100)
N439K
N439K & D614G (catalog numbers SP005-100, SP105-100)
N439K, D614G
B.1.1.7 (first identified in the U.K.; catalog numbers SP006-100, SP106-100)
Δ69-70, Δ144, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H
B.1.351 (first identified in South Africa; catalog numbers SP007-100, SP107-100)
L18F, D80A, D215G,Δ242-244, K417N, E484K, N501Y, D614G, A701V
P.1 (first identified in Brazil; catalog numbers SP008-100, SP108-100)
L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, T1027I, V1176F
B.1.617.1 (first identified in India; catalog numbers SP009-100, SP109-100)
G142D, E154K, L452R, E484Q, D614G, P681R, Q1071H
B.1.427/B.1.429 (first identified in California, USA; catalog numbers SP010-100, SP110-100)
S13I, W152C, L452R, D614G
B.1.617.2 (first identified in India; catalog numbers SP011-100, SP111-100)
T19R, G142D, 156del, 157del, R158G, L452R, T478K, D614G, P681R, D950N
Lambda C.37 (first identified in Peru; catalog numbers SP012-100, SP112-100)
G75V, T76I, del246/253, L452Q, F490S, D614G, T859N
Mu B.1.621 (first identified in Columbia; catalog numbers SP013-100, SP113-100)
T95I, Y144S, Y145N, R346K, E484K, N501Y, D614G, P681H, D950N
Omicron B.1.1.529 (first identified in South Africa; catalog numbers SP015-100, SP115-100)
A67V, Del 69-70HV, T95I, G142D, Del 143-145VYY, N211I, del 212L, insert215-217 EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H, N764K, D796Y, N856K, Q954H, N969K, L981F
Omicron BA.2 (first identified in South Africa; catalog numbers SP016-100, SP116-100)
T19I, L24S, del25/27, G142D, V213G, G339D, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, S477N, T478K, E484A, Q493R, Q498R, N501Y, Y505H, D614G, H655Y, N679K, P681H, N764K, D796Y, Q954H, N969K
Omicron BA.2.12.1 (first identified in South Africa; catalog numbers SP017-100, SP117-100)
T19I, L24S, del25/27, G142D, V213G, G339D, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, L452Q, S477N, T478K, E484A, Q493R, Q498R, N501Y, Y505H, D614G, H655Y, N679K, P681H, S704L, N764K, D796Y, Q954H, N969K
Omicron BA.4/BA.5/BA.5.1 (first identified in South Africa; catalog numbers SP018-100, SP118-100)
T19I, L24S, del25/27, del69/70, G142D, V213G, G339D, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, L452R, S477N, T478K, E484A, F486V, Q498R, N501Y, Y505H, D614G, H655Y, N679K, P681H, N764K, D796Y, Q954H, N969K
Omicron BA.4.1 (first identified in South Africa; catalog numbers SP019-100, SP119-100)
V3G, T19I, L24S, del25/27, del69/70, G142D, V213G, G339D, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, L452R, S477N, T478K, E484A, F486V, Q498R, N501Y, Y505H, D614G, H655Y, N679K, P681H, N764K, D796Y, Q954H, N969K
Omicron BF.7 (first identified in South Africa; catalog numbers SP020-100, SP120-100)
T19I, L24S, del25/27, del69/70, G142D, V213G, G339D, R346T, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, L452R, S477N, T478K, E484A, F486V, Q498R, N501Y, Y505H, D614G, H655Y, N679K, P681H, N764K, D796Y, Q954H, N969K
Omicron BQ.1 (first identified in South Africa; catalog numbers SP021-100, SP121-100)
T19I, L24S, del25/27, del69/70, G142D, V213G, G339D, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, K444T, L452R, N460K, S477N, T478K, E484A, F486V, Q498R, N501Y, Y505H, D614G, H655Y, N679K, P681H, N764K, D796Y, Q954H, N969K
Omicron BQ.1.1 (first identified in South Africa; catalog numbers SP022-100, SP122-100)
T19I, L24S, del25/27, del69/70, G142D, V213G, G339D, R346T, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, K444T, L452R, N460K, S477N, T478K, E484A, F486V, Q498R, N501Y, Y505H, D614G, H655Y, N679K, P681H, N764K, D796Y, Q954H, N969K
Omicron XBB (first identified in South Africa; catalog numbers SP023-100, SP123-100)
T19I, L24S, del25/27, V83A, G142D, del144/144, H146Q, Q183E, V213E, G339H, R346T, L368I, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, V445P, G446S, N460K, S477N, T478K, E484A, F486S, F490S, Q498R, N501Y, Y505H, D614G, H655Y, N679K, P681H, N764K, D796Y, Q954H, N969K
Omicron XBB.1 (first identified in South Africa; catalog numbers SP024-100, SP124-100)
T19I, L24S, del25/27, V83A, G142D, del144/144, H146Q, Q183E, V213E, G252V, G339H, R346T, L368I, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, V445P, G446S, N460K, S477N, T478K, E484A, F486S, F490S, Q498R, N501Y, Y505H, D614G, H655Y, N679K, P681H, N764K, D796Y, Q954H, N969K
Omicron XBB.1.5 (first identified in South Africa; catalog numbers SP025-100, SP125-100)
T19I, L24S, del25/27, V83A, G142D, del144/144, H146Q, Q183E, V213E, G252V, G339H, R346T, L368I, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, V445P, G446S, N460K, S477N, T478K, E484A, F486P, F490S, Q498R, N501Y, Y505H, D614G, H655Y, N679K, P681H, N764K, D796Y, Q954H, N969K
您可以从下表中订购这些细胞系。
除以下产品外,我们还提供稳定细胞系的定制。更多有关信息,请联系请联系GeneCopoeia技术支持support@igenebio.com或拨打4006-020-200
订购
货号
细胞系
描述
细胞类型
Selection marker
价格(¥)
SL221
HEK293T
HEK293T细胞稳定表达人ACE2 (NM_021804.2),2×106 cells
Embryonic kidney
Hygromycin
询价
SL222
HEK293T
HEK293T细胞稳定表达人ACE2 (NM_021804.2)和TMPRSS2 (NM_005656.3),2×106 cells
Embryonic kidney
询价
图1. GeneCopoeia稳定细胞系中ACE2和TMPRSS2的相对mRNA表达水平。利用对每个基因特异的引物,通过RT-qPCR扩增mRNA。ACE2和TMPRSS2的Ct值被归一化为管家基因GAPDH的Ct值。相对表达水平根据2^ΔΔCt计算。A. SL221,ACE2 mRNA在亲本细胞系HEK293T和过表达HEK293T细胞系中的表达。B. SL222,ACE2和TMPRSS2 mRNA在亲本细胞系HEK293T中的表达和ACE2-和TMPRSS2-过表达细胞系。
图2. 表达ACE2的HEK293T细胞(Cat.#SL221)、表达ACE2/TMPRSS2的HEH293T细胞和HEK293T细胞的细胞表面上ACE2蛋白表达的免疫荧光(IF)分析。将未渗透的细胞固定、阻断并与抗ACE2的一抗孵育,然后洗涤,并与有荧光标记的二抗孵育,再次洗涤并用DAPI染色。
图3. 表达TMPRSS2的HEK293T细胞(Cat.#SL222)和HEK293T细胞的细胞表面上TMPRSS2蛋白表达的免疫荧光(IF)分析。将未渗透的细胞固定、阻断并与抗TMPRSS2的一抗孵育,然后洗涤,并与有荧光标记的二抗孵育,再次洗涤并用DAPI染色。
A.