GeneCopoeia 的Lentifect™ SARS-CoV-2 S 蛋白假慢病毒由特定优化的包装体系制备而成。以SARS-CoV-2 Spike糖蛋白替代慢病毒包装体系中的囊泡性口炎病毒G糖蛋白(VSV-G)为衣壳蛋白进行包装制备成“假病毒”,该病毒可有效感染含ACE2(SARS-CoV-2的人类受体)的细胞。(Ou, X., et al., 2020. Nature Communications doi: 10.1038/s41467-020-15562-9; Hoffman, M., et al., 2020. Cell doi: 10.1016/j.cell.2020.02.052)。
Lentifect™ SARS-CoV-2 S 蛋白假慢病毒应用:
- 预防SARS-CoV-2病毒感染的疫苗研制
- 抗SARS-CoV-2病毒中和抗体的作用和机制研究
- 开发抗病毒治疗药物
- 研究病毒-受体相互作用的机制
- 以新冠病毒S蛋白作为表面衣壳糖蛋白
- S蛋白多样性,全长、截短、点突变可供选择
- 提供eGFP和luc蛋白标记,同时满足不同研究需求
- 不具备致病性,安全可靠,只需P2实验室环境
订购信息
SARS-CoV-2 S 蛋白假慢病毒
可从下方的 “Spike突变位点列表”了解突变位点信息。
SARS-CoV-2 截短S蛋白慢病毒(去除了19个氨基酸内质网保留信号,体外级别)
Spike突变位点列表
GeneCopoeia使用GISAID数据库得到每个变异体中最常见的突变信息,从而设计我们的假病毒颗粒。这些突变分布见下表。 请注意,每个突变株都有不同的亚型,以至于可能与我们所提供的假病毒颗粒的突变位点略有不同。如果您想定制与我们目前提供的不同的突变株假病毒,请联系我们4006-020-200或sales@igenebio.com。SARS-CoV-2 variant | Spike mutations |
Wild type (catalog numbers SP001-100, SP101-100) | None |
D614G (catalog numbers SP003-100, SP103-100) | D614G |
N439K (catalog numbers SP004-100, SP104-100) | N439K |
N439K & D614G (catalog numbers SP005-100, SP105-100) | N439K, D614G |
B.1.1.7 (first identified in the U.K.; catalog numbers SP006-100, SP106-100) | Δ69-70, Δ144, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H |
B.1.351 (first identified in South Africa; catalog numbers SP007-100, SP107-100) | L18F, D80A, D215G,Δ242-244, K417N, E484K, N501Y, D614G, A701V |
P.1 (first identified in Brazil; catalog numbers SP008-100, SP108-100) | L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, T1027I, V1176F |
B.1.617.1 (first identified in India; catalog numbers SP009-100, SP109-100) | G142D, E154K, L452R, E484Q, D614G, P681R, Q1071H |
B.1.427/B.1.429 (first identified in California, USA; catalog numbers SP010-100, SP110-100) | S13I, W152C, L452R, D614G |
B.1.617.2 (first identified in India; catalog numbers SP011-100, SP111-100) | T19R, G142D, 156del, 157del, R158G, L452R, T478K, D614G, P681R, D950N |
Lambda C.37 (first identified in Peru; catalog numbers SP012-100, SP112-100) | G75V, T76I, del246/253, L452Q, F490S, D614G, T859N |
Mu B.1.621 (first identified in Columbia; catalog numbers SP013-100, SP113-100) | T95I, Y144S, Y145N, R346K, E484K, N501Y, D614G, P681H, D950N |
Omicron B.1.1.529 (first identified in South Africa; catalog numbers SP015-100, SP115-100) | A67V, Del 69-70HV, T95I, G142D, Del 143-145VYY, N211I, del 212L, insert215-217 EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H, N764K, D796Y, N856K, Q954H, N969K, L981F |
Omicron BA.2 (first identified in South Africa; catalog numbers SP016-100, SP116-100) | T19I, L24S, del25/27, G142D, V213G, G339D, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, S477N, T478K, E484A, Q493R, Q498R, N501Y, Y505H, D614G, H655Y, N679K, P681H, N764K, D796Y, Q954H, N969K |
Omicron BA.2.12.1 (first identified in South Africa; catalog numbers SP017-100, SP117-100) | T19I, L24S, del25/27, G142D, V213G, G339D, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, L452Q, S477N, T478K, E484A, Q493R, Q498R, N501Y, Y505H, D614G, H655Y, N679K, P681H, S704L, N764K, D796Y, Q954H, N969K |
Omicron BA.4/BA.5/BA.5.1 (first identified in South Africa; catalog numbers SP018-100, SP118-100) | T19I, L24S, del25/27, del69/70, G142D, V213G, G339D, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, L452R, S477N, T478K, E484A, F486V, Q498R, N501Y, Y505H, D614G, H655Y, N679K, P681H, N764K, D796Y, Q954H, N969K |
Omicron BA.4.1 (first identified in South Africa; catalog numbers SP019-100, SP119-100) | V3G, T19I, L24S, del25/27, del69/70, G142D, V213G, G339D, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, L452R, S477N, T478K, E484A, F486V, Q498R, N501Y, Y505H, D614G, H655Y, N679K, P681H, N764K, D796Y, Q954H, N969K |
Omicron BF.7 (first identified in South Africa; catalog numbers SP020-100, SP120-100) | T19I, L24S, del25/27, del69/70, G142D, V213G, G339D, R346T, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, L452R, S477N, T478K, E484A, F486V, Q498R, N501Y, Y505H, D614G, H655Y, N679K, P681H, N764K, D796Y, Q954H, N969K |
Omicron BQ.1 (first identified in South Africa; catalog numbers SP021-100, SP121-100) | T19I, L24S, del25/27, del69/70, G142D, V213G, G339D, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, K444T, L452R, N460K, S477N, T478K, E484A, F486V, Q498R, N501Y, Y505H, D614G, H655Y, N679K, P681H, N764K, D796Y, Q954H, N969K |
Omicron BQ.1.1 (first identified in South Africa; catalog numbers SP022-100, SP122-100) | T19I, L24S, del25/27, del69/70, G142D, V213G, G339D, R346T, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, K444T, L452R, N460K, S477N, T478K, E484A, F486V, Q498R, N501Y, Y505H, D614G, H655Y, N679K, P681H, N764K, D796Y, Q954H, N969K |
Omicron XBB (first identified in South Africa; catalog numbers SP023-100, SP123-100) | T19I, L24S, del25/27, V83A, G142D, del144/144, H146Q, Q183E, V213E, G339H, R346T, L368I, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, V445P, G446S, N460K, S477N, T478K, E484A, F486S, F490S, Q498R, N501Y, Y505H, D614G, H655Y, N679K, P681H, N764K, D796Y, Q954H, N969K |
Omicron XBB.1 (first identified in South Africa; catalog numbers SP024-100, SP124-100) | T19I, L24S, del25/27, V83A, G142D, del144/144, H146Q, Q183E, V213E, G252V, G339H, R346T, L368I, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, V445P, G446S, N460K, S477N, T478K, E484A, F486S, F490S, Q498R, N501Y, Y505H, D614G, H655Y, N679K, P681H, N764K, D796Y, Q954H, N969K |
Omicron XBB.1.5 (first identified in South Africa; catalog numbers SP025-100, SP125-100) | T19I, L24S, del25/27, V83A, G142D, del144/144, H146Q, Q183E, V213E, G252V, G339H, R346T, L368I, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, V445P, G446S, N460K, S477N, T478K, E484A, F486P, F490S, Q498R, N501Y, Y505H, D614G, H655Y, N679K, P681H, N764K, D796Y, Q954H, N969K |
表达ACE2和TMPRSS2的HEK293T细胞系
GeneCopoeia已生产出单独表达人ACE2或ACE2与TMPRSS2联合表达的HEK293T细胞系,TMPRSS2是一种促进Spike-ACE2受体相互作用的蛋白酶。这些细胞系高水平表达ACE2和/或TMPRRSS2 mRNA(图1)。由于细胞表面显示ACE2蛋白(图2)和/或TMPRSS2蛋白(图3),这些细胞易于被其包膜中表达SARS-CoV-2 S蛋白的病毒感染。
您可以从下表中订购这些细胞系。
除以下产品外,我们还提供稳定细胞系的定制。更多有关信息,请联系请联系GeneCopoeia技术支持support@igenebio.com或拨打4006-020-200
欲了解更多信息或询价,请联系GeneCopoeia技术支持support@igenebio.com或拨打4006-020-200
Warranty statement and limited use license
购买须知
性能数据

图1. GeneCopoeia稳定细胞系中ACE2和TMPRSS2的相对mRNA表达水平。利用对每个基因特异的引物,通过RT-qPCR扩增mRNA。ACE2和TMPRSS2的Ct值被归一化为管家基因GAPDH的Ct值。相对表达水平根据2^ΔΔCt计算。A. SL221,ACE2 mRNA在亲本细胞系HEK293T和过表达HEK293T细胞系中的表达。B. SL222,ACE2和TMPRSS2 mRNA在亲本细胞系HEK293T中的表达和ACE2-和TMPRSS2-过表达细胞系。

图2. 表达ACE2的HEK293T细胞(Cat.#SL221)、表达ACE2/TMPRSS2的HEH293T细胞和HEK293T细胞的细胞表面上ACE2蛋白表达的免疫荧光(IF)分析。将未渗透的细胞固定、阻断并与抗ACE2的一抗孵育,然后洗涤,并与有荧光标记的二抗孵育,再次洗涤并用DAPI染色。

图3. 表达TMPRSS2的HEK293T细胞(Cat.#SL222)和HEK293T细胞的细胞表面上TMPRSS2蛋白表达的免疫荧光(IF)分析。将未渗透的细胞固定、阻断并与抗TMPRSS2的一抗孵育,然后洗涤,并与有荧光标记的二抗孵育,再次洗涤并用DAPI染色。
您可以从下表中订购这些细胞系。
除以下产品外,我们还提供稳定细胞系的定制。更多有关信息,请联系请联系GeneCopoeia技术支持support@igenebio.com或拨打4006-020-200
订购 | 货号 | 细胞系 | 描述 | 细胞类型 | Selection marker | 价格(¥) |
SL221 | HEK293T | HEK293T细胞稳定表达人ACE2 (NM_021804.2),2×106 cells | Embryonic kidney | Hygromycin | 询价 | |
SL222 | HEK293T | HEK293T细胞稳定表达人ACE2 (NM_021804.2)和TMPRSS2 (NM_005656.3),2×106 cells | Embryonic kidney | mCherry/ Puromycin, Hygromycin | 询价 |
|
性能数据

图1. GeneCopoeia稳定细胞系中ACE2和TMPRSS2的相对mRNA表达水平。利用对每个基因特异的引物,通过RT-qPCR扩增mRNA。ACE2和TMPRSS2的Ct值被归一化为管家基因GAPDH的Ct值。相对表达水平根据2^ΔΔCt计算。A. SL221,ACE2 mRNA在亲本细胞系HEK293T和过表达HEK293T细胞系中的表达。B. SL222,ACE2和TMPRSS2 mRNA在亲本细胞系HEK293T中的表达和ACE2-和TMPRSS2-过表达细胞系。

图2. 表达ACE2的HEK293T细胞(Cat.#SL221)、表达ACE2/TMPRSS2的HEH293T细胞和HEK293T细胞的细胞表面上ACE2蛋白表达的免疫荧光(IF)分析。将未渗透的细胞固定、阻断并与抗ACE2的一抗孵育,然后洗涤,并与有荧光标记的二抗孵育,再次洗涤并用DAPI染色。

图3. 表达TMPRSS2的HEK293T细胞(Cat.#SL222)和HEK293T细胞的细胞表面上TMPRSS2蛋白表达的免疫荧光(IF)分析。将未渗透的细胞固定、阻断并与抗TMPRSS2的一抗孵育,然后洗涤,并与有荧光标记的二抗孵育,再次洗涤并用DAPI染色。
性能数据
GeneCopoeia的研发团队测试了SARS-CoV-2 S 蛋白假慢病毒感染ACE2表达细胞的能力,如下图4所示。
A.

B.

C.

图4. 用表达eGFP/hLuc的SARS-CoV-2 S假慢病毒(假病毒)转导表达ACE2-或ACE2/TMPRSS2的HEK293T细胞。用10 μl假病毒在96孔板中转导每个细胞系。转导72小时后,在荧光显微镜下对细胞进行拍照,随后测量荧光素酶活性或进行FACS分选。SL221:表达ACE2的HEK293T细胞。SL222:表达ACE2和TMPRSS2的HEK293T细胞。SP101:全长S蛋白,D614变体。SP103:全长S蛋白,D614G变体。SP001:截短S蛋白,D614变体。SP003:截短S蛋白,D614G变体。A. 荧光显微镜。B. 荧光素酶活性测定。使用GeneCopoeia的Luc-Pair™ Firefly Luciferase HS Assay Kit(货号LF007),从假病毒转导细胞培养物中提取20 μl细胞裂解液,分析萤火虫荧光素酶活性,用FACS分选法测定转导的单位滴度units/ml。
A.

B.

C.

图4. 用表达eGFP/hLuc的SARS-CoV-2 S假慢病毒(假病毒)转导表达ACE2-或ACE2/TMPRSS2的HEK293T细胞。用10 μl假病毒在96孔板中转导每个细胞系。转导72小时后,在荧光显微镜下对细胞进行拍照,随后测量荧光素酶活性或进行FACS分选。SL221:表达ACE2的HEK293T细胞。SL222:表达ACE2和TMPRSS2的HEK293T细胞。SP101:全长S蛋白,D614变体。SP103:全长S蛋白,D614G变体。SP001:截短S蛋白,D614变体。SP003:截短S蛋白,D614G变体。A. 荧光显微镜。B. 荧光素酶活性测定。使用GeneCopoeia的Luc-Pair™ Firefly Luciferase HS Assay Kit(货号LF007),从假病毒转导细胞培养物中提取20 μl细胞裂解液,分析萤火虫荧光素酶活性,用FACS分选法测定转导的单位滴度units/ml。
相关产品
相关产品及服务
资源
在线研讨会
标题:来自GeneCopoeia的COVID-19冠状病毒研究的先进工具 2020年9月9日,星期三
由SARS-CoV-2冠状病毒引起的新冠肺炎全球大流行凸显了对诊断试剂、抗病毒治疗和疫苗开发的迫切需求。在本次网络研讨会中,我们将讨论GeneCopoeia先进的解决方案,包括检测试剂盒、SARS-CoV-2假病毒以及研究对抗这种致命疾病所需的其他工具。
观看录制的网络研讨会/下载幻灯片
已发表的文章
查看近期使用GeneCopoeia SARS-CoV-2 S蛋白假慢病毒产品的已发表文章
2022
在线研讨会
标题:来自GeneCopoeia的COVID-19冠状病毒研究的先进工具 2020年9月9日,星期三
由SARS-CoV-2冠状病毒引起的新冠肺炎全球大流行凸显了对诊断试剂、抗病毒治疗和疫苗开发的迫切需求。在本次网络研讨会中,我们将讨论GeneCopoeia先进的解决方案,包括检测试剂盒、SARS-CoV-2假病毒以及研究对抗这种致命疾病所需的其他工具。
观看录制的网络研讨会/下载幻灯片
已发表的文章
查看近期使用GeneCopoeia SARS-CoV-2 S蛋白假慢病毒产品的已发表文章
2022
- Hwang, J-Y., et al. (2022). Humoral and Cellular Responses to COVID-19 Vaccines in SARS-CoV-2 Infection-Naïve and -Recovered Korean Individuals. Vaccines doi: 10.3390/vaccines10020332 [ACE2-expressing HEK293T stable cell line]
- Park, H., et al. (2022). TMED3 Complex Mediates ER Stress-Associated Secretionof CFTR, Pendrin, and SARS-CoV-2 Spike. Advanced Science doi: 10.1002/advs.202105320 [ACE2- and TMPRSS2-expressing HEK293T stable cell line]
- Sim, J-R., et al. (2022). Amelioration of SARS-CoV-2 infection by ANO6 phospholipid scramblase inhibition. Cell Reports doi: 10.1016/j.celrep.2022.111117 [ACE2- and TMPRSS2-expressing HEK293T stable cell line]
- Grodzki, M., et al. (2022). Genome-scale CRISPR screens identify host factors that promote human coronavirus infection. Genome Medicine doi: 10.1186/s13073-022-01013-1 [ACE2-expressing HEK293T stable cell line]
- Ariumi, Y. (2022). Host Cellular RNA Helicases Regulate SARS-CoV-2 Infection. Journal of Virology doi: 10.1128/jvi.00002 [ACE2-expressing HEK293T stable cell line]
- Pivniouk, V., et al. (2022). The OM-85 bacterial lysate inhibits SARS-CoV-2 infection of epithelial cells by downregulating SARS-CoV-2 receptor expression. Journal of Allergy and Clinical Immunology doi: 10.1016/j.jaci.2021.11.019 [ACE2-expressing HEK293T stable cell line]
- Travis, B.J., et al. (2022). Significance of chlorine-dioxide-based oral rinses in preventing SARS-CoV-2 cell entry. Oral Diseases doi: 10.1111/odi.14319 [ACE2-expressing HEK293T stable cell line]
- Hashizume, M., et al. (2021). Population-Specific ACE2 Single-Nucleotide Polymorphisms Have Limited Impact on SARS-CoV-2 Infectivity In Vitro. Viruses doi: 10.3390/v13010067 [ACE2-expressing HEK293T stable cell line]
- Ao, Z., et al .(2021). Identification and evaluation of the inhibitory effect of Prunella vulgaris extract on SARS-coronavirus 2 virus entry. PLOS ONE doi: 10.1371/journal.pone.0251649 [ACE2-expressing HEK293T stable cell line]
- Paidi, R.K., et al. (2021). Eugenol, a Component of Holy Basil (Tulsi) and Common Spice Clove, Inhibits the Interaction Between SARS-CoV-2 Spike S1 and ACE2 to Induce Therapeutic Responses. Journal of Neuroimmune Pharmacology doi: 10.1007/s11481-021-10028-1 [ACE2-expressing HEK293T stable cell line]
- Paidi, R.K., et al. (2021). Selective Inhibition of the Interaction between SARS-CoV-2 Spike S1 and ACE2 by SPIDAR Peptide Induces Anti-Inflammatory Therapeutic Responses. Journal of Immunology doi: 10.4049/jimmunol.2100144 [ACE2-expressing HEK293T stable cell line]
- Reznikov, L.R., et al. (2020). Identification of antiviral antihistamines for COVID-19 repurposing. Biochemical and Biophysical Research Communications doi: 10.1016/j.bbrc.2020.11.095 [ACE2-expressing HEK293T stable cell line]