– 高灵敏性萤火虫荧光素酶检测试剂盒
Luc-Pair™ Firefly Luciferase HS Assay Kit 是一款高灵敏性荧光素酶检测试剂盒,可高效、快速检测萤火虫(Firefly luciferase,FLuc)荧光素酶的活性。由于其具有极高的灵敏度,可检测极低水平表达、调控和转染不佳的细胞的表达,也可搭配微孔板使用。适用于 GeneCopoeia 及 Promega 的萤火虫荧光素酶表达载体。
产品特点
• 灵敏性高—本试剂盒对萤火虫荧光素酶产生的光信号极度敏感;
• 适用范围广—适用于多种不同的真核生物( 脊椎动物、低等无脊椎动物)细胞系样品在微孔板(如:96孔板) 或单管中检测;
• 背景值低—背景更低,数据更准确;
• 操作简便—实验步骤简便;
• 重复性好—检测数据更可靠,且荧光素酶的浓度线性范围超过 9 个数量级。
同时,GeneCopoeia 还提供 Luc-Pair™ Duo-Luciferase Assay Kit 2.0(高稳定性双荧光素酶检测试剂盒)和 Luc-Pair™ Duo-Luciferase HS Assay Kit(高灵敏性双荧光素酶检测试剂盒)。
图1. 用 GeneCopoeia 的 Luc-Pair™ 高灵敏性萤火虫荧光素酶检测试剂盒检测萤火虫荧光素酶的活性。用 Promega 的 pGL4.13 报告载体转染 HEK293 细胞,转染 48 小时后检测荧光信号,结果如上图所示。Fluc-GeneCopoeia 代表 GeneCopoeia 的检测结果, Fluc-Promega 代表 Promega 品牌试剂盒的检测结果。
图2. 用 GeneCopoeia 的 Luc-Pair™ 高灵敏性萤火虫荧光素酶检测试剂盒检测萤火虫荧光素酶信号的稳定性。用 Promega 的 pGL4.13 报告载体转染 HEK293 细胞,转染 48 小时后检测荧光信号,FLuc(FLuc-GeneCopoeia)的活性如上图所示。用自动加样器分配 Fluc 工作液,设置震动混合为 1 秒,然后立即检测,检测时间为 20 秒。
购买 | 货号 | 产品 | 描述 | 价格(¥) |
ZX001 | Fluc + Rluc reporter clone | pEZX-FR01 包含萤火虫荧光素酶(Fluc)和海肾荧光素酶(Rluc)的报告载体 | 3960 | |
ZX002 | Fluc + Rluc reporter clone | pEZX-FR02 包含萤火虫荧光素酶(Fluc)和海肾荧光素酶(Rluc)的报告载体 | 3960 | |
ZX003 | Fluc + Rluc reporter clone | pEZX-FR03 包含萤火虫荧光素酶(Fluc)和海肾荧光素酶(Rluc)的报告载体 | 3960 |
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