Lentifect™慢病毒
慢病毒载体是将目的基因序列导入普通细胞与难转染细胞中的一种强有力手段。慢病毒通过感染细胞,将目的基因转导至靶细胞的基因组,使目的基因在活体及体外实验均能持续表达。为获得高滴度和稳定均一的慢病毒,慢病毒的包装、纯化和滴度测定都需要较长时间和足够的经验,而GeneCopoeia拥有专业的慢病毒包装团队,保证产品的高质量。 Lentifect™ 纯化慢病毒既提供预制产品,也可根据客户需求进行定制。慢病毒生产经过标准的实验流程,使用高质量质粒DNA提取试剂盒、EndoFectin™ 转染试剂及TiterBoost™ 慢病毒滴度增强试剂。高质量的试剂和GeneCopoeia的专业技能可保证高滴度的慢病毒出品。
Lentifect™ 慢病毒产品优势:
- 慢病毒可即时用于实验,节约时间与成本;
- 严格的质量控制,保证高品质与高滴度;
- 可有效转导目的基因至难转染细胞和动物模型。
GeneCopoeia eGFP慢病毒感染H1299细胞
图1. 于24孔板中,使用图示用量的eGFP纯化慢病毒(catalog number LP303)感染H1299细胞(ATCC),添加5 µg/ml polybrene试剂。感染72小时后,使用荧光显微镜观察eGFP表达情况。
GeneCopoeia mCherry慢病毒感染H1299细胞
图2. 于24孔板中,使用图示用量的eGFP纯化慢病毒(catalog number LP151)感染H1299细胞(ATCC),添加5 µg/ml polybrene试剂。感染72小时后,使用荧光显微镜观察mCherry表达情况。
预制慢病毒产品
纯化慢病毒产品包括以下几种,可立即用于实验:
- 阳性和阴性慢病毒对照:eGFP, eYFP, eCFP, mCherry, PLUM, Firefly luciferase, Renilla luciferase, Gaussia luciferase
- 慢病毒载体的ORF表达克隆:可从数百种低价的慢病毒载体ORF克隆中获得您的目的基因。
- CRISPR相关预制慢病毒:Cas9 nuclease,sgRNA for PD-1、PD-L1、GFP、HUWE and EMX1.
- 诱导IPSC重编程预制慢病毒: c-Myc, Klf4, Oct4, Sox2, Nanog, Lin28A, CRE-excisable CMYC, CRE-excisable GLIS1.
- 细胞永生化预制慢病毒:SV40 small T & large T antigen, SV40 large T antigen, hTERT, CDK4, HOX, c-Myc, Bmi1 and HPV-16 E6-E7.
- 外泌体标记预制慢病毒:CD9, CD81 and CD63.
- 其它常用预制慢病毒:HIV tat, Cell structure lentivirus, CRE recombinase and FLP recombinase.
纯化慢病毒滴度可达108 TU/ ml。Lentifect™慢病毒产品均经过严格的质量控制,插入序列均经过测序验证和qPCR滴度验证。H1299细胞的转导效率可通过检测eGFP或mCherry的表达量获得。
若您需要的慢病毒不在以上表格,请浏览特殊慢病毒产品或定制慢病毒服务。
您所需的慢病毒不在以上表格,或者您想了解更多再下单吗? 可直接致电 4006-020-200 / (020) 28069233 或发送邮件至 sales@igenebio.com 技术支持第一时间与您联系,并为您完成详细查找!
下载 |
Lentifect™ Lentiviral Particles Cancellation, QA, License, and Warranty |
Positive and negative control lentiviral particle datasheets |
特殊慢病毒产品
– 价格低、周期短
特殊慢病毒产品——按照插入片段分类:
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插入人ORF序列的OmicsLink™ 慢病毒载体pReceiver-Lv105 |
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包含人、小鼠及大鼠miRNA的miExpress™ 慢病毒载体pEZX-MR03 |
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包含人、小鼠和大鼠miRNA 抑制子的miArrest™ 慢病毒载体pEZX-AM03 |
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包含人类癌症相关的miRNA或其抑制子的慢病毒载体pEZX-MR03 或 pEZX-AM03 |
发货周期:2-4周。 自行搜索太繁冗?以下还有方法轻松下单。 拿起电话,致电 4006-020-200 / (020) 28069233 或发送邮件至 sales@igenebio.com 技术支持第一时间与您联系,并为您完成详细查找。 查找交给我们,慢病毒交给您! 如需了解更多信息,请浏览预制慢病毒产品或定制慢病毒服务。
客户定制慢病毒服务 |
GeneCopoeia为您提供包括ORF、 cDNA、 shRNA、miRNA前体和miRNA抑制剂在内的慢病毒包装服务,多种包含或不含融合标签的表达载体可供选择。 GeneCopoeia的专业团队包装高质量纯化慢病毒,病毒滴度可达10^8 TU/ml。 服务周期根据服务内容不同而有所差异,最短为20个工作日,最终产品规格为200 µl、400 µl 或1 ml。 如需定制慢病毒,请点击此处,填写服务表格。
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- Li, X., et al. (2023). Ultrasensitive sensors reveal the spatiotemporal landscape of lactate metabolism in physiology and disease. Cell Metabolism doi: 10.1016/j.cmet.2022.10.002 [Lenti-Pac™ lentivirus concentration kit]
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- Yoshizue, T., et al. (2023). Antisera Produced Using an E. coli-Expressed SARS-CoV-2 RBD and Complemented with a Minimal Dose of Mammalian-Cell-Expressed S1 Subunit of the Spike Protein Exhibits Improved Neutralization. Int J Mol Sci doi: 10.3390/ijms241310583[Lenti-Pac™ SARS-CoV-2 Truncated Spike protein-pseudotyped (D614G) Lentivirus Packaging Kit]
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- Wei, Y., et al. (2023). LncRNA XIST promotes adjuvant-induced arthritis by increasing the expression of YY1 via miR-34a-5p. Arch Rheumatol doi: 10.46497/ArchRheumatol.2022.9250 [miExpress™ inhibitor clone; OMICSLINK™ lentiviral shRNA clone; OMICSLINK™ ORF expression clones; Lenti-Pac™ HIV Expression Packaging Kit]
- Huang, X., et al. (2023). Downregulation of LncRNA GCLC-1 Promotes Microcystin-LR-Induced Malignant Transformation of Human Liver Cells by Regulating GCLC Expression. Toxics doi: 10.3390/toxics11020162 [OMICSLINK™ shRNA clones targeting LOC101927136; Lenti-Pac™ HIV Expression Packaging Kit]
- Mansouri, M., et al. (2023). Protein Networks Associated with Native Metabotropic Glutamate 1 Receptors (mGlu1) in the Mouse Cerebellum. Cells doi: 10.3390/cells12091325 [OMICSLINK™ mouse Glu1α ORF expression clone; OMICSLINK™ mouse KCTD12 lentiviral ORF expression clone]
- Parween, F., et al. (2023). Chemokine positioning determines mutually exclusive roles for their receptors in extravasation of pathogenic human T cells. bioRxiv doi: 10.1101/2023.01.25.525561 [Lentifect™ lentiviral particles]
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- Kim, G.S., et al. (2023). Single-cell analysis identifies Ifi27l2a as a novel gene regulator of microglial inflammation in the context of aging and stroke. Res Sq doi: 10.21203/rs.3.rs-2557290/v1 [Lentifect™ lentiviral particles]
- Zhu, Y., et al. (2023). Research on Radiosensitivity of the Protein Kinase B Signaling Pathway in Cervical Cancer. Comput Math Methods Med doi: 10.1155/2021/6350038 [Lenti-Pac™ HIV Expression Packaging Kit; OMICSLINK™ shRNA clone]
- Liu, Q., et al. (2023). A long non-coding RNA H19/microRNA-138/TLR3 network is involved in high phosphorus-mediated vascular calcification and chronic kidney disease. Cell Cycle doi: 10.1080/15384101.2022.2064957 [Lentifect™ lentiviral particles]
- Li, X., et al. (2023). Beclin1- and Atg13-dependent autophagy activation and morroniside have synergistic effect on osteoblastogenesis. Exp Biol Med (Maywood) doi: 10.1177/15353702221116879 [Lenti-Pac™ HIV Expression Packaging Kit; OMICSLINK™ Lentiviral ORF expression clone]
- Han, W., et al. (2023). Exploiting the tumor-suppressive activity of the androgen receptor by CDK4/6 inhibition in castration-resistant prostate cancer. Mol Ther doi: 10.1016/j.ymthe.2022.01.039 [Lentifect™ shRNA lentiviral particles]
- Hasan, K.M., et al. (2023). Fatty Acid Excess Dysregulates CARF to Initiate the Development of Hepatic Steatosis. Cells doi: 10.3390/cells12071069 [AAVPrime™ AAV particles expressing CARF and AAV-empty vector; OMICSLINK™ CARF Lentiviral ORF expression clone]
- Zhang, X., et al. (2023). IGFBP3 induced by the TGF-β/EGFRvIII transactivation contributes to the malignant phenotype of glioblastoma. iScience doi: 10.1016/j.isci.2023.106639 [shIGFBP3 lentiviral particles and control (psi-LvRU6rLP) ; Lentifect™ shRNA lentiviral particles]
- Lang, L., et al. (2023). Adaptive c-Met-PLXDC2 Signaling Axis Mediates Cancer Stem Cell Plasticity to Confer Radioresistance-associated Aggressiveness in Head and Neck Cancer. Cancer Res Commun doi: 10.1158/2767-9764.CRC-22-0289 [Lenti-Pac™ 293Ta Cell Line]
- Georgakopoulos-Soares, L., et al. (2023). Transcription factor binding site orientation and order are major drivers of gene regulatory activity. Nat Commun doi: 10.1038/s41467-023-37960-5 [Lenti-Pac™ HIV Expression Packaging Kit]
- Huang, W-J., et al. (2023). The β-catenin-LINC00183-miR-371b-5p-Smad2/LEF1 axis promotes adult T-cell lymphoblastic lymphoma progression and chemoresistance. J Exp Clin Cancer Res doi: 10.1186/s13046-023-02670-9 [8 mg/mL polybrene]
- Shi, Y., et al. (2023). PRMT3-mediated arginine methylation of IGF2BP1 promotes oxaliplatin resistance in liver cancer. Nat Commun doi: 10.1038/s41467-023-37542-5 [dCas9 lentivirus]
- Jiang, L., et al. (2023). Chaperoning of specific tau structure by immunophilin FKBP12 regulates the neuronal resilience to extracellular stress. Sci Adv doi: 10.1126/sciadv.add9789 [OMICSLINK™ NEUROG2 Lentiviral ORF expression clone]
- Zhang, R., et al. (2023). Mesenchymal stem cell suppresses the efficacy of CAR-T toward killing lymphoma cells by modulating the microenvironment through stanniocalcin-1. eLife doi: 10.7554/eLife.82934 [Lenti-Pac™ HIV expression packaging kit]
- Deng,Y., et al. (2023). NUF2 Promotes Breast Cancer Development as a New Tumor Stem Cell Indicator. Int J Mol Sci doi: 10.3390/ijms24044226 [Lenti-Pac™ HIV qRT-PCR Titration Kit]
- Stierschneider, A., et al. (2023). Comparative and Temporal Characterization of LPS and Blue-Light-Induced TLR4 Signal Transduction and Gene Expression in Optogenetically Manipulated Endothelial Cells. Cells doi: 10.3390/cells12050697 [Lenti-Pac™ 293Ta Cell Line]
- Koesterich, J., et al. (2023). Characterization of De Novo Promoter Variants in Autism Spectrum Disorder with Massively Parallel Reporter Assays. Int J Mol Sci doi: 10.3390/ijms24043509 [Lenti-PAC™ HIV Expression Packaging Kit]
- Jia, Y., et al. (2023). Tripartite motif containing 69 elicits ERK2-dependent EYA4 turnover to impart pancreatic tumorigenesis. J Cancer doi: 10.7150/jca.79905 [TRIM69 sgRNA lentivirus]
- Luo, F., et al. (2023). Hypermethylation of HIC2 is a potential prognostic biomarker and tumor suppressor of glioma based on bioinformatics analysis and experiments. CNS Neurosci Ther doi: 10.1111/cns.14093 [Lenti-PAC™ HIV Expression Packaging Kit]
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- Fuller, M.J., et al. (2023). Investigating role of ASIC2 in synaptic and behavioral responses to drugs of abuse. Front Mol Biosci doi: 10.3389/fmolb.2023.1118754 [Lentifect™ Lentiviral particles expressing Asic2a and Asic2b]
- Huang, Z., et al. (2023). CaMKII may regulate renal tubular epithelial cell apoptosis through YAP/NFAT2 in acute kidney injury mice. Ren Fail doi: 10.1080/0886022X.2023.2172961 [Lenti-Pac™ HIV Expression Packing Kit]
- Zhang, C., et al. (2023). Carbonic Anhydrase IX Controls Vulnerability to Ferroptosis in Gefitinib-Resistant Lung Cancer. Oxid Med Cell Longev doi: 10.1155/2023/1367938 [lentiviral vector]
- Zhang, Y., et al. (2023). Extracellular Vesicles Expressing CD19 Antigen Improve Expansion and Efficacy of CD19-Targeted CAR-T Cells. Int J Nanomedicine doi: 10.2147/IJN.S390720 [lentiviral vector pCDH-CMV-CD19]
- Pallichankandy, S., et al. (2023). Targeting oxeiptosis-mediated tumor suppression: a novel approach to treat colorectal cancers by sanguinarine. Cell Death Discov doi: 10.1038/s41420-023-01376-3 [Lenti-Pac™ Expression Packaging Kit]
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- Zhang, R., et al. (2023). Knockdown SENP1 Suppressed the Angiogenic Potential of Mesenchymal Stem Cells by Impacting CXCR4-Regulated MRTF-A SUMOylation and CCN1 Expression. Biomedicines doi: 10.3390/biomedicines11030914 [Lenti-Pac™ HIV Expression Packaging Kit]
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- Nieddu, V., et al. (2023). Matrix Gla Protein drives stemness and tumor initiation in ovarian cancer. Cell Death Dis doi: 10.1038/s41419-023-05760-w [OMICSLINK™ MGP ORF expression clone]
- Wang, S., et al. (2023). Myc derived circRNA promotes triple-negative breast cancer progression via reprogramming fatty acid metabolism. Discov Oncol doi: 10.1007/s12672-023-00679-2 [lentivirus vector psi-LVRU6GP]
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