• 易锦生物提供的所有人、小鼠和大鼠ORF 克隆及其慢病毒、AAV 颗粒与重组蛋白均使用基于毛细管电泳的 Sanger 法“金标准”全长测序，保证氨基酸序列与NCBI 数据库一致。
    All human and mice ORF clones and related Lentivirus, AAV particles and recombinant proteins provided by iGeneBio are fully sequenced using Sanger sequencing by capillary electrophoresis and Amino Acid Sequences are guaranteed to be matched with NCBI database.
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COVID-19 慢病毒 & AAV

GeneCopoeia有使用慢病毒和腺相关病毒(AAV)进行基因传递的全套优质的产品。

慢病毒的解决方法：

  • 慢病毒质粒​，可用于表达ORF cDNAs、基因启动子、miRNA前体和抑制剂、CRISPR sgRNA和 shRNA，第三代慢病毒包装。
  • 预制慢病毒，用于感染大多数哺乳动物细胞（包病毒包膜质粒为VSV-G基因），或用于感染表达ACE2细胞（包病毒包膜质粒为 SARS-CoV-2 S基因）。
  • 包装试剂盒， 用VSV-G基因作为包膜质粒的慢病毒包装试剂盒和用SARS-CoV-2 S作为包包质粒的慢病毒包装试剂盒

AAV解决方案：

  • 预制病毒， 用于多种血清型中许多哺乳动物细胞类型的感染。
  • 血清型检测包， 帮你确定哪种AAV血清型最适合感染你的目的细胞。

表达SARS-CoV-2病毒蛋白的慢病毒

用于表达SARS-CoV-2病毒蛋白的慢病毒颗粒有两个主要用途:

1、Lentifect™ SARS-CoV-2 S 蛋白假慢病毒，目前常用的慢病毒是由病毒包膜中表达囊泡状气孔病毒G糖蛋白(VSV-G)产生的，这类慢病毒能够广泛进入哺乳动物细胞。然而在慢病毒包装过程中，编码VSV-G的包装质粒可以被表达SARS-CoV-2 S 蛋白的质粒所替代，这类病毒针对性的感染表达ACE2的细胞，而ACE2是人类SARS-CoV-2感染受体 (Ou, X., et al., 2020. Nature Communications doi: 10.1038/s41467-020-15562-9; Hoffman, M., et al., 2020. Cell doi: 10.1016/j.cell.2020.02.052)。这些病毒可用于疫苗开发和阻止SARS-CoV-2进入细胞的抗体测试。如果你想订购带有SARS-CoV-2 S蛋白的假慢病毒颗粒，请联系我们。

2、Expression of SARS-CoV-2 病毒蛋白慢病毒（VSV-G​标准型），我们有80多种慢病毒载体类型，要订购表达SARS-CoV-2病毒蛋白的高滴度、即用慢病毒颗粒，请从下表中选择产品ID。

Product ID Protein accession Symbol Alias Description
NV201 YP_009724389.1 orf1ab GU280_gp01 orf1ab polyprotein [Severe acute respiratory syndrome coronavirus 2}, full-length gene
NV202 YP_009725297.1 orf1ab GU280_gp01 leader protein [Severe acute respiratory syndrome coronavirus 2], mature peptide*
NV203 YP_009725298.1 orf1ab GU280_gp01 nsp2n [Severe acute respiratory syndrome coronavirus 2], mature peptide*
NV204 YP_009725299.1 orf1ab GU280_gp01 nsp3n [Severeacute respiratory syndrome coronavirus 2], mature peptide*
NV205 YP_009725300.1 orf1ab GU280_gp01 nsp4n [Severe acute respiratory syndrome coronavirus 2], mature peptide*
NV206 YP_009725301.1 orf1ab GU280_gp01 3C-like proteinasen [Severe acute respiratory syndrome coronavirus 2], mature peptide*
NV207 YP_009725302.1 orf1ab GU280_gp01 nsp6n [Severe acute respiratory syndrome coronavirus 2], mature peptide*
NV208 YP_009725303.1 orf1ab GU280_gp01 nsp7n [Severe acute respiratory syndrome coronavirus 2], mature peptide*
NV209 YP_009725304.1 orf1ab GU280_gp01 nsp8n [Severe acute respiratory syndrome coronavirus 2], mature peptide*
NV210 YP_009725305.1 orf1ab GU280_gp01 nsp9n [Severe acute respiratory syndrome coronavirus 2], mature peptide*
NV211 YP_009725306.1 orf1ab GU280_gp01 nsp10n [Severe acute respiratory syndrome coronavirus 2], mature peptide*
NV212 YP_009725307.1 orf1ab GU280_gp01 RNA-dependent RNA polymerasen [Severe acute respiratory syndrome coronavirus 2], mature peptide*
NV213 YP_009725308.1 orf1ab GU280_gp01 helicasen [Severe acute respiratory syndrome coronavirus 2], mature peptide*
NV214 YP_009725309.1 orf1ab GU280_gp01 3′-to-5′ exonucleasen [Severe acute respiratory syndrome coronavirus 2], mature peptide*
NV215 YP_009725310.1 orf1ab GU280_gp01 endoRNAsen [Severe acute respiratory syndrome coronavirus 2], mature peptide*
NV216 YP_009725311.1 orf1ab GU280_gp01 2′-O-ribose methyltransferasen [Severe acute respiratory syndrome coronavirus 2], mature peptide*
NV217 YP_009725295.1 orf1a GU280_gp01 orf1a polyproteinn [Severe acute respiratory syndrome coronavirus 2], full-length gene
NV218 YP_009725312.1 orf1a GU280_gp01 produced by pp1a onlyn [Severe acute respiratory syndrome coronavirus 2], mature peptide*
NV219 YP_009724390.1 S GU280_gp02 surface glycoprotein;structural protein; spike proteinn [Severe acute respiratory syndrome coronavirus 2], full-length gene
NV220 YP_009724390.1 S1 GU280_gp02 surface glycoprotein subunit 1 [Severe acute respiratory syndrome coronavirus 2], mature peptide
NV221 YP_009724390.1 S2 GU280_gp02 surface glycoprotein subunit 2 [Severe acute respiratory syndrome coronavirus 2], mature peptide*
NV222 YP_009724390.1 RBD GU280_gp02 Receptor binding domain [Severe acute respiratory syndrome coronavirus 2], mature peptide*
NV223 YP_009724391.1 ORF3a GU280_gp03 ORF3a protein [Severe acute respiratory syndrome coronavirus 2], full-length gene
NV224 YP_009724392.1 E GU280_gp04 envelope protein [Severe acute respiratory syndrome coronavirus 2], full-length gene
NV225 YP_009724393.1 M GU280_gp05 membrane glycoprotein [Severe acute respiratory syndrome coronavirus 2], full-length gene
NV226 YP_009724394.1 ORF6 GU280_gp06 ORF6 protein [Severe acute respiratory syndrome coronavirus 2], full-length gene
NV227 YP_009724395.1 ORF7a GU280_gp07 ORF7a protein [Severe acute respiratory syndrome coronavirus 2], full-length gene
NV228 YP_009725318.1 ORF7b GU280_gp08 ORF7b [Severe acute respiratory syndrome coronavirus 2], full-length gene
NV229 YP_009724396.1 ORF8 GU280_gp09 ORF8 protein [Severe acute respiratory syndrome coronavirus 2], full-length gene
NV230 YP_009724397.2 N GU280_gp10 nucleocapsid phosphoprotein [Severe acute respiratory syndrome coronavirus 2], full-length gene
NV231 YP_009725255.1 ORF10 GU280_gp11 ORF10 protein [Severe acute respiratory syndrome coronavirus 2], full-length gene

*注：成熟肽是前体蛋白的加工而成。为了使这些蛋白在细胞中表达，在ORF序列的5’端添加了ATG。

表达SARS-CoV-2宿主蛋白的慢病毒

预制表达ACE2慢病毒

订购 货号 规格 滴度 启动子 筛选标记 标记 载体 价格
LPP-U1285-Lv152-100 100 μl >108 TU/mL纯化 CMV
Hygromycin
 无标记 pReceiver-Lv152 2000

 

Warranty statement and limited use license

定制表达ACE2的慢病毒

  • 可为你提供80多种载体类型的慢病毒颗粒来选择表达ACE2。

定制表达TMPRSS2的慢病毒

  • 可提供80多种载体类型的慢病毒颗粒来选择表达TMPRSS2。

 

AAV

可提供80多种载体类型的的AAV颗粒来选择表达目的基因。

  • 定制表达ACE2 的AAV颗粒
  • 定制表达TMPRSS2 的AAV颗粒
  • 定制表达SARS-CoV-2病毒蛋白的AAV颗粒
 

我们也提供其它冠状病毒（SARS-CoV、MERS-CoV）蛋白的慢病毒和AAV解决方案。

 

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