GeneCopoeia有使用慢病毒和腺相关病毒(AAV)进行基因传递的全套优质的产品。
慢病毒的解决方法:
- 慢病毒质粒,可用于表达ORF cDNAs、基因启动子、miRNA前体和抑制剂、CRISPR sgRNA和 shRNA,第三代慢病毒包装。
- 预制慢病毒,用于感染大多数哺乳动物细胞(包病毒包膜质粒为VSV-G基因),或用于感染表达ACE2细胞(包病毒包膜质粒为 SARS-CoV-2 S基因)。
- 包装试剂盒, 用VSV-G基因作为包膜质粒的慢病毒包装试剂盒和用SARS-CoV-2 S作为包包质粒的慢病毒包装试剂盒
AAV解决方案:
- 预制病毒, 用于多种血清型中许多哺乳动物细胞类型的感染。
- 血清型检测包, 帮你确定哪种AAV血清型最适合感染你的目的细胞。
表达SARS-CoV-2病毒蛋白的慢病毒
用于表达SARS-CoV-2病毒蛋白的慢病毒颗粒有两个主要用途:
1、Lentifect™ SARS-CoV-2 S 蛋白假慢病毒,目前常用的慢病毒是由病毒包膜中表达囊泡状气孔病毒G糖蛋白(VSV-G)产生的,这类慢病毒能够广泛进入哺乳动物细胞。然而在慢病毒包装过程中,编码VSV-G的包装质粒可以被表达SARS-CoV-2 S 蛋白的质粒所替代,这类病毒针对性的感染表达ACE2的细胞,而ACE2是人类SARS-CoV-2感染受体 (Ou, X., et al., 2020. Nature Communications doi: 10.1038/s41467-020-15562-9; Hoffman, M., et al., 2020. Cell doi: 10.1016/j.cell.2020.02.052)。这些病毒可用于疫苗开发和阻止SARS-CoV-2进入细胞的抗体测试。如果你想订购带有SARS-CoV-2 S蛋白的假慢病毒颗粒,请联系我们。
2、Expression of SARS-CoV-2 病毒蛋白慢病毒(VSV-G标准型),我们有80多种慢病毒载体类型,要订购表达SARS-CoV-2病毒蛋白的高滴度、即用慢病毒颗粒,请从下表中选择产品ID。
Product ID | Protein accession | Symbol | Alias | Description |
NV201 | YP_009724389.1 | orf1ab | GU280_gp01 | orf1ab polyprotein [Severe acute respiratory syndrome coronavirus 2}, full-length gene |
NV202 | YP_009725297.1 | orf1ab | GU280_gp01 | leader protein [Severe acute respiratory syndrome coronavirus 2], mature peptide* |
NV203 | YP_009725298.1 | orf1ab | GU280_gp01 | nsp2n [Severe acute respiratory syndrome coronavirus 2], mature peptide* |
NV204 | YP_009725299.1 | orf1ab | GU280_gp01 | nsp3n [Severeacute respiratory syndrome coronavirus 2], mature peptide* |
NV205 | YP_009725300.1 | orf1ab | GU280_gp01 | nsp4n [Severe acute respiratory syndrome coronavirus 2], mature peptide* |
NV206 | YP_009725301.1 | orf1ab | GU280_gp01 | 3C-like proteinasen [Severe acute respiratory syndrome coronavirus 2], mature peptide* |
NV207 | YP_009725302.1 | orf1ab | GU280_gp01 | nsp6n [Severe acute respiratory syndrome coronavirus 2], mature peptide* |
NV208 | YP_009725303.1 | orf1ab | GU280_gp01 | nsp7n [Severe acute respiratory syndrome coronavirus 2], mature peptide* |
NV209 | YP_009725304.1 | orf1ab | GU280_gp01 | nsp8n [Severe acute respiratory syndrome coronavirus 2], mature peptide* |
NV210 | YP_009725305.1 | orf1ab | GU280_gp01 | nsp9n [Severe acute respiratory syndrome coronavirus 2], mature peptide* |
NV211 | YP_009725306.1 | orf1ab | GU280_gp01 | nsp10n [Severe acute respiratory syndrome coronavirus 2], mature peptide* |
NV212 | YP_009725307.1 | orf1ab | GU280_gp01 | RNA-dependent RNA polymerasen [Severe acute respiratory syndrome coronavirus 2], mature peptide* |
NV213 | YP_009725308.1 | orf1ab | GU280_gp01 | helicasen [Severe acute respiratory syndrome coronavirus 2], mature peptide* |
NV214 | YP_009725309.1 | orf1ab | GU280_gp01 | 3'-to-5' exonucleasen [Severe acute respiratory syndrome coronavirus 2], mature peptide* |
NV215 | YP_009725310.1 | orf1ab | GU280_gp01 | endoRNAsen [Severe acute respiratory syndrome coronavirus 2], mature peptide* |
NV216 | YP_009725311.1 | orf1ab | GU280_gp01 | 2'-O-ribose methyltransferasen [Severe acute respiratory syndrome coronavirus 2], mature peptide* |
NV217 | YP_009725295.1 | orf1a | GU280_gp01 | orf1a polyproteinn [Severe acute respiratory syndrome coronavirus 2], full-length gene |
NV218 | YP_009725312.1 | orf1a | GU280_gp01 | produced by pp1a onlyn [Severe acute respiratory syndrome coronavirus 2], mature peptide* |
NV219 | YP_009724390.1 | S | GU280_gp02 | surface glycoprotein;structural protein; spike proteinn [Severe acute respiratory syndrome coronavirus 2], full-length gene |
NV220 | YP_009724390.1 | S1 | GU280_gp02 | surface glycoprotein subunit 1 [Severe acute respiratory syndrome coronavirus 2], mature peptide |
NV221 | YP_009724390.1 | S2 | GU280_gp02 | surface glycoprotein subunit 2 [Severe acute respiratory syndrome coronavirus 2], mature peptide* |
NV222 | YP_009724390.1 | RBD | GU280_gp02 | Receptor binding domain [Severe acute respiratory syndrome coronavirus 2], mature peptide* |
NV223 | YP_009724391.1 | ORF3a | GU280_gp03 | ORF3a protein [Severe acute respiratory syndrome coronavirus 2], full-length gene |
NV224 | YP_009724392.1 | E | GU280_gp04 | envelope protein [Severe acute respiratory syndrome coronavirus 2], full-length gene |
NV225 | YP_009724393.1 | M | GU280_gp05 | membrane glycoprotein [Severe acute respiratory syndrome coronavirus 2], full-length gene |
NV226 | YP_009724394.1 | ORF6 | GU280_gp06 | ORF6 protein [Severe acute respiratory syndrome coronavirus 2], full-length gene |
NV227 | YP_009724395.1 | ORF7a | GU280_gp07 | ORF7a protein [Severe acute respiratory syndrome coronavirus 2], full-length gene |
NV228 | YP_009725318.1 | ORF7b | GU280_gp08 | ORF7b [Severe acute respiratory syndrome coronavirus 2], full-length gene |
NV229 | YP_009724396.1 | ORF8 | GU280_gp09 | ORF8 protein [Severe acute respiratory syndrome coronavirus 2], full-length gene |
NV230 | YP_009724397.2 | N | GU280_gp10 | nucleocapsid phosphoprotein [Severe acute respiratory syndrome coronavirus 2], full-length gene |
NV231 | YP_009725255.1 | ORF10 | GU280_gp11 | ORF10 protein [Severe acute respiratory syndrome coronavirus 2], full-length gene |
*注:成熟肽是前体蛋白的加工而成。为了使这些蛋白在细胞中表达,在ORF序列的5'端添加了ATG。
表达SARS-CoV-2宿主蛋白的慢病毒
预制表达ACE2慢病毒
AAV
可提供80多种载体类型的的AAV颗粒来选择表达目的基因。
- 定制表达ACE2 的AAV颗粒
- 定制表达TMPRSS2 的AAV颗粒
- 定制表达SARS-CoV-2病毒蛋白的AAV颗粒
我们也提供其它冠状病毒(SARS-CoV、MERS-CoV)蛋白的慢病毒和AAV解决方案。