产品介绍
miRNA抑制剂可以特异、高效地抑制生物体内源性miRNA的活性,是研究miRNA功能缺失的重要工具。GeneCopoeia提供慢病毒或非病毒载体的miArrest™ miRNA抑制剂表达克隆和化学合成的miRNA抑制剂。 | |
miArrest™ miRNA抑制剂克隆可以特异性地结合目的miRNA,瞬时和稳定地抑制miRNA的功能,采用H1或者U6启动子驱动转录可以保证在大多数哺乳动物细胞中高水平表达。 |
优势
覆盖面广
- 针对所有已知的人、小鼠和大鼠miRNA
灵活的载体选择
- 慢病毒载体可转导普通细胞和难转染、未分化细胞
- 非病毒载体可进行稳定或瞬时转导
先进可靠的设计
- 表达框经过专利算法设计并经过严格的实验验证
抑制性能优越
- 与其他化学合成的miRNA抑制剂相比具有更强、更持久的抑制作用和极低的细胞毒性(参照表1)
- 采用H1或U6启动子保证在大多数哺乳动物细胞中高水平表达
高通量兼容
- 快速简便的实验设计
- 可同时检测多个样
HEK293细胞中共转染miR-125a 抑制剂表达质粒 (GeneCopoeia HmiR-AN0094-AM01) 、miR-125a 前体表达质粒(GeneCopoeia HmiR0309-MR03) 和miR-125a的靶标克隆质粒(GeneCopoeia HmiT019205-MT02:将LIN28 的3’UTR克隆到Gaussia荧光素酶-碱性磷酸酶双报告载体)。转染24小时后检测报告基因Gaussia 荧光素酶和参照基因碱性磷酸酶的活性。只转染Gluc-LIN28-3’-UTR质粒的细胞中Gaussia 荧光素酶与碱性磷酸酶活性比值设为1 (左数第一条柱),用来标准化其他细胞样品中的报告基因活性。结果表明miR-125a抑制了Gluc-LIN28-3’-UTR中Gluc 70%以上的活性(左数第二条柱)。这种抑制效应可被导入的不同剂量的miR-125a抑制剂表达质粒特异性的阻遏。转染100 ng 抑制剂表达质粒时,细胞内报告基因/参照基因活性比值(右数第一条)已超出单独转染Gluc-LIN28-3’-UTR报告质粒的对照组。这说明miR-125a抑制剂可以阻遏外源和内源性的miR-125a对靶标基因的抑制,提高GLuc-LIN28-3’-UTR转录本的翻译水平。
基于克隆载体的抑制剂 | 2′-甲氧修饰的抑制剂 | 化学合成的LNA抑制剂 | |
抑制效力 | +++++ | ++ | ++ |
特异性 | +++++ | +++ | +++ |
稳定性 | +++++ | + | +++ |
持久性 | Long term | Transient | Transient |
细胞毒性 | – | – | + |
导入细胞难易程度 | +++++ | – | – |
表1. 基于克隆表达载体的和化学合成的miRNA抑制剂的比较
图2. miRNA抑制剂表达克隆的作用机理
miRNA 抑制剂克隆导入细胞后表达的miRNA抑制剂(miArrest)特异地结合它们的靶miRNA。miRNA抑制剂转录后折叠形成一个稳定的陷阱结构,可以结合两分子靶miRNA。 miArrest™高效、特异地结合细胞内成熟的靶miRNA,阻止了miRNA对靶标mRNA的降解或者翻译抑制,上调miRNA靶标基因的表达。 独特的载体设计确保最大的特异性、抑制强度、抑制作用持久度和最低的脱靶效应。 |
载体
miRNA抑制剂表达克隆载体
载体 | 报告基因 | 筛选标记 | 启动子 | 载体类型 |
---|---|---|---|---|
pEZX-AM01 | mCherry | Puromycin | H1 | Mammalian |
pEZX-AM02 | mCherry | Puromycin | U6 | Mammalian |
pEZX-AM03 | mCherry | Hygromycin | H1 | HIV Lentiviral |
pEZX-AM04 | mCherry | Hygromycin | U6 | HIV Lentiviral |
pEZX-AM04p | mCherry | Puromycin | U6 | HIV Lentiviral |
miRNA抑制剂表达AAV载体
载体 | 报告基因 | 筛选标记 | 启动子 | 载体类型 |
---|---|---|---|---|
pEZX-AM06 | mCherry | Hygromycin | H1 | AAV |
pEZX-AM07 | mCherry | Hygromycin | U6 | AAV |
慢病毒载体的miRNA抑制剂表达克隆 |
GeneCopoeia提供客户定制的慢病毒载体miRNA抑制剂表达克隆,周期2-5周。
化学合成的miRNA抑制剂
需要优化设计的miRNA抑制剂,点击了解更多
产品 | 规格 |
---|---|
Synthetic miRNA mimics | 5 nmol |
Synthetic miRNA controls | 1.25 nmol |
Synthetic miRNA inhibitors | 10 nmol |
Synthetic miRNA inhibitor controls | 2.5 noml |
克隆套装
miRNA抑制剂表达克隆套装
GeneCopoeia除了提供单个克隆,也提供预制和定制的miRNA抑制剂表达克隆套装。了解详情请点击联系我们或拨打400-6020-200。
载体 | 载体类型 | 启动子 | 筛选标记 | 标签 |
---|---|---|---|---|
pEZX-AM03 | Mammalian expression (lentiviral) | H1 | Hygromycin | mCherry |
如果您有什么问题,请致电4006-020-200,或发邮件至sales@igenebio.com。
载体: |
Human whole genome miRNA inhibitorclone set (Lentiviral)
货号 | 物种 | 产品 | miRNA | 价格 |
---|---|---|---|---|
MIC-HG-AM03 | Human | Human whole genome miRNA inhibitor clone set (lentiviral) | 1177 | Inquire |
Custom order | Various | Custom-selected | Various | Inquire |
Human cancer miRNA inhibitor clone sets (Lentiviral)
货号 | 物种 | 产品 | 癌 | miRNA | 价格 |
---|---|---|---|---|---|
MIC-HCY-AM03 | Human | Human bladder cancer miRNA inhibitor clone set | Bladder cancer | 63 | Inquire |
MIC-HCN-AM03 | Human | Human brain cancer miRNA inhibitor clone set | Brain cancer | 206 | Inquire |
MIC-HCB-AM03 | Human | Human breast cancer miRNA inhibitor clone set | Breast cancer | 158 | Inquire |
MIC-HCV-AM03 | Human | Human cervical cancer miRNA inhibitor clone set | Cervical cancer | 26 | Inquire |
MIC-HCI-AM03 | Human | Human cholangiocarcinoma miRNA inhibitor clone set | Cholangiocarcinoma | 25 | Inquire |
MIC-HCC-AM03 | Human | Human colorectal cancer miRNA inhibitor clone set | Colorectal cancer | 175 | Inquire |
MIC-HCW-AM03 | Human | Human endometrial cancer miRNA inhibitor clone set | Endometrial cancer | 86 | Inquire |
MIC-HCG-AM03 | Human | Human gastric cancer miRNA inhibitor clone set | Gastric cancer | 76 | Inquire |
MIC-HCT-AM03 | Human | Human head and neck cancer miRNA inhibitor clone set | Head and Neck cancer | 188 | Inquire |
MIC-HCH-AM03 | Human | Human hepatocellular carcinoma miRNA inhibitor clone set | Hepatocellular Carcinoma | 170 | Inquire |
MIC-HCK-AM03 | Human | Human kidney cancer miRNA inhibitor clone set | Kidney cancer | 67 | Inquire |
MIC-HCX-AM03 | Human | Human leukemia miRNA inhibitor clone set | Leukemia | 163 | Inquire |
MIC-HCL-AM03 | Human | Human lung cancer miRNA inhibitor clone set | Lung cancer | 178 | Inquire |
MIC-HCZ-AM03 | Human | Human lymphoma miRNA inhibitor clone set | Lymphoma | 112 | Inquire |
MIC-HCM-AM03 | Human | Human melanoma miRNA inhibitor clone set | Melanoma | 131 | Inquire |
MIC-HCE-AM03 | Human | Human mesothelioma miRNA inhibitor clone set | Mesothelioma | 21 | Inquire |
MIC-HCR-AM03 | Human | Human myeloma miRNA inhibitor clone set | Myeloma | 45 | Inquire |
MIC-HCO-AM03 | Human | Human ovarian cancer miRNA inhibitor clone set | Ovarian cancer | 174 | Inquire |
MIC-HCP-AM03 | Human | Human pancreatic cancer miRNA inhibitor clone set | Pancreatic cancer | 166 | Inquire |
MIC-HCA-AM03 | Human | Human pituitary adenoma miRNA inhibitor clone set | Pituitary Adenoma | 33 | Inquire |
MIC-HCQ-AM03 | Human | Human prostate cancer miRNA inhibitor clone set | Prostate cancer | 153 | Inquire |
MIC-HCS-AM03 | Human | Human sarcoma miRNA inhibitor clone set | Sarcoma | 27 | Inquire |
MIC-HCJ-AM03 | Human | Human thyroid carcinoma miRNA inhibitor clone set | Thyroid Carcinoma | 55 | Inquire |
MIC-HCU-AM03 | Human | Human uterine leiomyoma miRNA inhibitor clone set | Uterine Leiomyoma | 40 | Inquire |
Custom order | Various | Custom-selected | Various | Various | Inquire |
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