GeneCopoeia’s 提供的抗人前脑源性神经营养因子(Human proBDNF)抗体是由小鼠单克隆抗体制得。本抗体对人前脑源性神经营养因子(proBDNF)高度特异，不识别成熟的BDNF。
免疫原
纯化的GST-前区蛋白可用于制备免疫小鼠及筛选杂交瘤克隆。mAb287克隆从初期筛选获得，并使用相关的重组蛋白、神经元、脑细胞溶解产物及来自bdnf KO或野生型同窝仔畜（如下方数据所示）的切片作进一步鉴定。
特异性
本抗proBDNF抗体只识别人的前BDNF，不识别成熟的BDNF。
产品应用
Western blot、免疫共沉淀、免疫染色及亲和纯化。
Figure 1. 对抗前体抗体的鉴定. (a) Western blot蛋白印迹鉴定mAb287单克隆抗体的免疫特异性。运用SDSPAGE及带有抗成熟BDNF抗体。(N-20, Santa Cruz) (左泳道所示)的探针，及带有mAb287单克隆抗体的探针(右泳道所示)，将纯化的重组成熟BDNF(50 ng)和纯化的重组proBDNF(50 ng)分离。(b) 使用mAb287抗体对脑细胞溶解产物中的proBDNF进行检测。bdnf -/-、bdnf +/+ 和 bdnf-HA/HA 小鼠的海马体细胞溶解产物与mAb287进行免疫共沉淀。使用SDS-PAGE分离条带后，使用前文的抗mAb287、抗HA.11或抗成熟BDNF抗体对其进行免疫共沉淀。缺失的前体条带如图所标示，和理论条带大小一致，为14至16 kDa。
产品订购
| 购买 | 货号 | 产品 | 描述 | 价格（¥） | |
| AT021 | Anti-Human proBDNF Antibody | Mouse monoclonal antibody specific for the prodomain of human brain-derived neurotrophic factor (proBDNF). | 2700 | ||
已发表的文章
Wang, B.D., et al. (2022). miR-31 from Mesenchymal Stem Cell-Derived Extracellular Vesicles Alleviates Intervertebral Disc Degeneration by Inhibiting NFAT5 and Upregulating the Wnt/β-Catenin Pathway. Stem Cells Int doi: 10.1155/2022/2164057 [anti-NC; GL–02]
Smulders, L., et al. (2022). Phosphatidylinositol Monophosphates Regulate the Membrane Localization of HSPA1A, a Stress-Inducible 70-kDa Heat Shock Protein. Biomolecules doi: 10.3390/biom12060856 [OmicsLink™ monoclonal mouse anti-GFP antibody; OmicsLink™ anti-c-Myc antibody]
Mizui, T., et al. (2019). Cerebrospinal fluid BDNF pro-peptide levels in major depressive disorder and schizophrenia. Journal of Psychiatric Research doi: 10.1016/j.jpsychires.2019.03.024 [anti-human proBDNF antibody]
Benicky, J., et a.. (2019).N-Glycosylation is required for secretion of the precursor to brain-derived neurotrophic factor (proBDNF) carrying sulfated LacdiNAc structures. The Journal of Biological Chemistry doi: 10.1074/jbc.RA119.009989 [anti-human proBDNF antibody]
Yang, B., et al. (2017). Altered expression of BDNF, BDNF pro-peptide and their precursor proBDNF in brain and liver tissues from psychiatric disorders: rethinking the brain–liver axis. Translational Psychiatry doi: 10.1038/tp.2017.95 [anti-human proBDNF antibody]
Zhang, S., et al. (2017). Inhibition of BDNF production by MPP+ through up-regulation of miR-210-3p contributes to dopaminergic neuron damage in MPTP model. Neuorscience Letters doi: 10.1016/j.neulet.2017.10.014 [anti-human proBDNF antibody]
