• 复能易锦提供的全部人类和小鼠ORF克隆及相关慢病毒和AAV病毒，所提供的载体质粒均采用毛细管电泳sanger全长测序验证，并承诺编码的氨基酸序列与NCBI数据库匹配。
    All human and mice ORF clones and related Lentivirus, AAV particles and recombinant proteins provided by iGeneBio are fully sequenced using Sanger sequencing by capillary electrophoresis and Amino Acid Sequences are guaranteed to be matched with NCBI database.
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OmicsLink™ 即用型ORF表达克隆载体

  • 即用型表达，无需亚克隆步骤
  • 提供40,000多个测序验证过的人和小鼠基因
  • 100种以上的表达载体选择
  • 50种以上的融合标签（是否带有IRES请参看下表）
  • 提供Gateway PLUS™ 穿梭克隆
Need assistance selecting a vector system? Contact us here or email directly at inquiry@igenebio.com

 

 

 

 

 

表达克隆载体和Gateway PLUS™穿梭克隆载体选择

载体 启动子 宿主细胞 筛选标记 标签 蛋白酶位点
pShuttle™ N/A Gateway PLUS shuttle N/A Untagged N/A
pShuttle ™ N/A Gateway PLUS shuttle
(no stop codon)		 N/A Untagged N/A
载体 启动子 宿主细胞 筛选标记 标签 蛋白酶位点
pReceiver-B01 T7 Bacterial N/A N-His N/A
pReceiver-B31 T7 Bacterial N/A C-His N/A
pReceiver-B02 T7 Bacterial N/A Untagged N/A
pReceiver-B03 T7 Bacterial N/A N-GST Tev
pReceiver-B04 T7 Bacterial N/A N-GST EK
pReceiver-B05 Tac Bacterial N/A N-GST Tev
pReceiver-B06 Tac Bacterial N/A N-GST EK
pReceiver-B07 Tac Bacterial N/A N-MBP Tev
pReceiver-B08 Tac Bacterial N/A N-MBP EK
pReceiver-B09 T7 Bacterial N/A N-Avi N/A
pReceiver-B10 Tac Bacterial N/A N-Flag N/A
pReceiver-B11 Tac Bacterial N/A N-His N/A
pReceiver-B12 Tac Bacterial N/A His-SUMO Sumo protease
pReceiver-B13 T7 Bacterial N/A His-SUMO SUMO Protease
pReceiver-B80 T7 Bacterial N/A N-HaloTag Tev
载体 启动子 宿主细胞 筛选标记 标签 蛋白酶位点
pReceiver-M34 CAG Mammalian Neomycin C-Flag N/A
pReceiver-M35 CMV Mammalian Neomycin C-Flag N/A
pReceiver-M37 CAG Mammalian Neomycin C-eGFP N/A
pReceiver-M39 CMV Mammalian Puromycin C-Flag N/A
pReceiver-M62 CMV Mammalian Neomycin C-Avi-IRES-Biotin ligase N/A
pReceiver-M73 CMV Mammalian Neomycin C-Myc-IRES2-mCherry N/A
pReceiver-M83 CMV Mammalian Neomycin IRES2-mCherry N/A
pReceiver-M90 CMV Mammalian Puromycin SV40-eGFP-IRES-Puromycin N/A
pReceiver-M91 CMV Mammalian Puromycin C-3xHA-SV40-eGFP-IRES-Puromycin N/A
pReceiver-M92 CMV Mammalian Puromycin C-Myc-SV40-eGFP-IRES-Puromycin N/A
pReceiver-M93 CMV Mammalian Puromycin C-Flag-SV40-eGFP-IRES-Puromycin N/A
pReceiver-M94 EF1a Mammalian Puromycin Untagged N/A
pReceiver-M95 CMV Mammalian Puromycin SV40-mCherry-IRES-Puromycin N/A
pReceiver-M97 EF1a Mammalian Neomycin C-eGFP(monomeric) N/A
pReceiver-M99 CMV Mammalian Neomycin C-mEOS3 N/A
pReceiver-M01 CMV Mammalian Neomycin N-His N/A
pReceiver-M51 CMV Mammalian Neomycin C-His+IRES-eGFP N/A
pReceiver-M77 CMV Mammalian Neomycin C-His N/A
pReceiver-M02 CMV Mammalian Neomycin Untagged N/A
pReceiver-M67 CMV Mammalian Hygromycin Untagged N/A
pReceiver-M68 CMV Mammalian Puromycin Untagged N/A
pReceiver-M29 CMV Mammalian Neomycin N-eGFP N/A
pReceiver-M03 CMV Mammalian Neomycin C-eGFP N/A
pReceiver-M98 CMV Mammalian Neomycin C-eGFP(monomeric) N/A
pReceiver-M15 CMV Mammalian Neomycin N-eYFP N/A
pReceiver-M16 CMV Mammalian Neomycin C-eYFP N/A
pReceiver-M32 CMV Mammalian Neomycin N-eCFP N/A
pReceiver-M33 CMV Mammalian Neomycin C-eCFP N/A
pReceiver-M04 CMV Mammalian Neomycin N-GST EK
pReceiver-M05 CMV Mammalian Neomycin N-Avi N/A
pReceiver-M48 CMV Mammalian Neomycin N-Avi+IRES-Biotin ligase N/A
pReceiver-M17 CMV Mammalian Neomycin C-Avi N/A
pReceiver-M06 CMV Mammalian Neomycin N-3xHA N/A
pReceiver-M07 CMV Mammalian Neomycin C-3xHA N/A
pReceiver-M08 CMV Mammalian Neomycin C-3xHA-His N/A
pReceiver-M43 CMV Mammalian Neomycin N-Myc N/A
pReceiver-M45 CMV Mammalian Neomycin C-3xHA+IRES-eGFP N/A
pReceiver-M09 CMV Mammalian Neomycin C-Myc N/A
pReceiver-M10 CMV Mammalian Neomycin C-Myc-His N/A
pReceiver-M72 CMV Mammalian Neomycin C-Myc+IRES2-eGFP N/A
pReceiver-M11 CMV Mammalian Neomycin N-Flag N/A
pReceiver-M12 CMV Mammalian Neomycin N-3XFlag N/A
pReceiver-M46 CMV Mammalian Neomycin C-Flag+IRES-eGFP N/A
pReceiver-M14 CMV Mammalian Neomycin C-3XFlag N/A
pReceiver-M49 CMV Mammalian Neomycin N-HaloTag Tev protease
pReceiver-M50 CMV Mammalian Neomycin C-HaloTag Tev protease
pReceiver-M55 CMV Mammalian Neomycin N-mCherry N/A
pReceiver-M56 CMV Mammalian Neomycin C-mCherry N/A
pReceiver-M61 CMV Mammalian Neomycin IRES2-eGFP N/A
载体 启动子 宿主细胞 筛选标记 标签 蛋白酶位点
pReceiver-Lv117 CMV Mammalian Puromycin N-3xHA N/A
pReceiver-Lv118 CMV Mammalian Neomycin N-3xHA N/A
pReceiver-Lv145 EF1a Mammalian Hygromycin SV40-hygromycin N/A
pReceiver-Lv163 EF1a Mammalian Neomycin SV40-neomycin N/A
pReceiver-Lv170 CMV Mammalian Neomycin C-Myc N/A
pReceiver-Lv181 CMV Mammalian Puromycin N-3xFlag N/A
pReceiver-Lv185 PGK Mammalian Puromycin Untagged N/A
pReceiver-Lv188 CMV Mammalian N/A C-eGFP N/A
pReceiver-Lv193 CMV Mammalian Puromycin C-eGFP(monomeric) N/A
pReceiver-Lv195 EF1a Mammalian Puromycin SV40-puromycin N/A
pReceiver-Lv197 CMV Mammalian Blasticidin Untagged N/A
pReceiver-Lv218 CMV Mammalian Neomycin IRES2-Neomycin N/A
pReceiver-Lv219 CMV Mammalian Puromycin IRES2-Puromycin N/A
pReceiver-Lv221 CMV Mammalian N/A IRES2-eYFP N/A
pReceiver-Lv222 CMV Mammalian N/A IRES2-eCFP N/A
pReceiver-Lv233 EF1a Mammalian Puromycin SV40-eGFP-IRES-Puromycin N/A
pReceiver-Lv234 CAG Mammalian N/A IRES2-eGFP N/A
pReceiver-Lv235 CAG Mammalian Puromycin SV40-eGFP-IRES-Puromycin N/A
pReceiver-Lv236 CAG Mammalian Puromycin Untagged N/A
pReceiver-Lv237 CAG Mammalian Puromycin C-Flag-SV40-Puromycin N/A
pReceiver-Lv241 CMV Mammalian Puromycin C-Flag-SV40-Puromycin N/A
pReceiver-Lv243 CAG Mammalian Puromycin C-Avi-Flag-SV40-Puromycin N/A
pReceiver-Lv244 CMV Mammalian Hygromycin SV40-mCherry-IRES-Hygromycin N/A
pReceiver-Lv245 EF1a Mammalian Puromycin cMyc-SV40-mCherry-IRES-Puromycin N/A
pReceiver-Lv246 UBC Mammalian Puromycin SV40-eGFP-IRES-Puromycin N/A
pReceiver-Lv247 UBC Mammalian Puromycin IRES2-mCherry-IRES-Puromycin N/A
pReceiver-Lv251 CMV Mammalian Puromycin C-3XFlag-SV40-Puromycin N/A
pReceiver-Lv253 EF1a Mammalian Puromycin SV40-mcherry-IRES-Puromycin N/A
pReceiver-Lv254 EF1a Mammalian Neomycin SV40-mcherry-IRES-Neomycin N/A
pReceiver-Lv105 CMV Mammalian Puromycin Untagged N/A
pReceiver-Lv151 CMV Mammalian Neomycin Untagged N/A
pReceiver-Lv152 CMV Mammalian Hygromycin Untagged N/A
pReceiver-Lv153 CMV Mammalian N/A Untagged N/A
pReceiver-Lv186 CMV Mammalian N/A C-3xHA N/A
pReceiver-Lv157 CMV Mammalian Neomycin C-3xHA N/A
pReceiver-Lv120 CMV Mammalian Puromycin C-3xHA N/A
pReceiver-Lv108 CMV Mammalian Puromycin N-Avi N/A
pReceiver-Lv124 CMV Mammalian Puromycin C-Avi N/A
pReceiver-Lv242 CMV Mammalian Puromycin C-Avi-FLAG N/A
pReceiver-Lv154 CMV Mammalian Neomycin C-eCFP N/A
pReceiver-Lv127 CMV Mammalian Puromycin C-eCFP N/A
pReceiver-Lv109 CMV Mammalian Puromycin N-eCFP N/A
pReceiver-Lv103 CMV Mammalian Puromycin N-eGFP N/A
pReceiver-Lv122 CMV Mammalian Puromycin C-eGFP N/A
pReceiver-Lv182 CMV Mammalian Neomycin N-eGFP N/A
pReceiver-Lv183 CMV Mammalian Neomycin C-eGFP N/A
pReceiver-Lv189 CMV Mammalian N/A N-eGFP N/A
pReceiver-Lv123 CMV Mammalian Puromycin C-eYFP N/A
pReceiver-Lv104 CMV Mammalian Puromycin N-eYFP N/A
pReceiver-Lv187 CMV Mammalian N/A C-FLAG N/A
pReceiver-Lv101 CMV Mammalian Neomycin N-Flag N/A
pReceiver-Lv102 CMV Mammalian Puromycin N-Flag N/A
pReceiver-Lv158 CMV Mammalian Neomycin C-FLAG N/A
pReceiver-Lv203 CMV Mammalian Puromycin C-Flag-SV40-eGFP-IRES-puromycin N/A
pReceiver-Lv216 CMV Mammalian Puromycin C-Flag-SV40-mCherry-IRES-puromycin N/A
pReceiver-Lv129 CMV Mammalian Puromycin C-HaloTag Tev
pReceiver-Lv110 CMV Mammalian Puromycin N-HaloTag Tev
pReceiver-Lv211 CMV Mammalian Hygromycin C-HaloTag-IRES-hygromycin N/A
pReceiver-Lv128 CMV Mammalian Puromycin C-His N/A
pReceiver-Lv215 CMV Mammalian N/A IRES2-eGFP N/A
pReceiver-Lv205 CMV Mammalian Puromycin IRES2-eGFP-IRES-puromycin N/A
pReceiver-Lv220 CMV Mammalian Hygromycin IRES2-Hygromycin N/A
pReceiver-Lv214 CMV Mammalian N/A IRES2-mCherry N/A
pReceiver-Lv213 CMV Mammalian Puromycin IRES2-mCherry-IRES-puromycin N/A
pReceiver-Lv111 CMV Mammalian Puromycin N-mCherry N/A
pReceiver-Lv130 CMV Mammalian Puromycin C-mCherry N/A
pReceiver-Lv155 CMV Mammalian Neomycin C-mCherry N/A
pReceiver-Lv107 CMV Mammalian Puromycin N-Myc N/A
pReceiver-Lv125 CMV Mammalian Puromycin C-Myc N/A
pReceiver-Lv126 CMV Mammalian Puromycin C-Myc-His N/A
pReceiver-Lv202 CMV Mammalian Puromycin C-Myc-SV40-eGFP-IRES-puromycin N/A
pReceiver-Lv204 CMV Mammalian Puromycin C-Myc-SV40-mCherry-IRES-puromycin N/A
pReceiver-Lv207 CMV Mammalian Hygromycin SV40-eGFP-IRES-hygromycin N/A
pReceiver-Lv201 CMV Mammalian Puromycin SV40-eGFP-IRES-puromycin N/A
pReceiver-Lv217 CMV Mammalian Puromycin SV40-hLUC-IRES-puromycin N/A
pReceiver-Lv206 CMV Mammalian Puromycin SV40-mCherry-IRES-puromycin N/A
pReceiver-Lv171 EF1a Mammalian N/A IRES-eGFP N/A
pReceiver-Lv173 EF1a Mammalian N/A IRES-mcherry N/A
pReceiver-Lv180 EF1a Mammalian Puromycin IRES-mcherry-SV40-puromycin N/A
pReceiver-Lv179 EF1a Mammalian Puromycin IRES-eGFP-SV40-puromycin N/A
pReceiver-Lv228 PGK Mammalian N/A IRES2-eGFP N/A
pReceiver-Lv229 PGK Mammalian N/A IRES2-mcherry N/A
pReceiver-Lv230 PGK Mammalian Puromycin IRES2-mcherry-IRES-puromycin N/A
pReceiver-Lv231 PGK Mammalian Puromycin IRES2-eGFP-IRES-puromycin N/A
pLX304 CMV Mammalian Blasticidin C-V5 N/A

AAV病毒载体  (17种)

GeneCopoeia提供以下ORF AAV病毒载体，所有载体均适用于搭配不同血清型进行包装。载体可包装的最大片段长度为3 kb。

除了下方即用型表达载体，您还可以使用 V-Architect工具，在我们的启动子和标签元件库中自由组装，设计符合您实验所需的克隆质粒。

载体名称 启动子 启动子介绍 报告基因 可包装片段长度
pEZ-AV02 EF-1α 也是应用范围较广的启动子。在活体实验的表现基本优于CMV。 N/A 3.2 kb
pEZ-AV04 CAG 在活体实验中常用的强启动子。 N/A 2.0 kb
pEZ-AV06 CBH 强启动子，且表达时间较长。 N/A 2.8 kb
pEZ-AV07 CMV 强启动子，应用范围较广。 IRES-eGFP 1.3 kb
pEZ-AV08 EF-1α 也是应用范围较广的启动子。在活体实验的表现基本优于CMV。 IRES-eGFP 2.1 kb
pEZ-AV09 CAG 在活体实验中常用的强启动子。 IRES-eGFP 0.9 kb
pEZ-AV11 CBH 强启动子，且表达时间较长。 IRES-eGFP 1.7 kb
pEZ-AV12 CAMKlla 在大脑皮层和海马体中的兴奋性神经元有特异性表达。 N/A 2.4 kb
pEZ-AV13 hSYN 人源synapsin-1基因启动子，是神经元特异启动子。 N/A 3.2 kb
pEZ-AV15 MHC 一种人源的肌肉特异启动子。 N/A 3.3 kb
pEZ-AV18 MCK 一种人源的心肌特异性启动子。 N/A 3.0 kb
pEZ-AV24 MBP 在中枢神经系统少突细胞中特异性表达。 N/A 3.0 kb
pEZ-AV33 ALB 一种人源的肝脏特异性启动子。 N/A 2.0 kb
pEZ-AV40 CMV 强启动子，应用范围较广。 C-P2A-eGFP 2.6 kb
pEZ-AV41 EF1a 也是应用范围较广的启动子。在活体实验的表现基本优于CMV。 C-P2A-eGFP 2.45 kb
pEZ-AV42 CAG 在活体实验中常用的强启动子。 C-P2A-eGFP 1.3 kb
pEZ-AV43 CMV 强启动子，应用范围较广。 N/A 3.45 kb

 

载体 启动子 宿主细胞 筛选标记 标签 蛋白酶位点
pReceiver-Y01 GAL1 Yeast N/A C-His N/A
pReceiver-YAD pADH Yeast N/A GAL4AD N/A
pReceiver-YBD pADH Yeast N/A GAL4DB N/A
载体 启动子 宿主细胞 筛选标记 标签 蛋白酶位点
pReceiver-I01 AcMNPV polyhedrin Insect cell N/A N-His Tev
载体 启动子 宿主细胞 筛选标记 标签 蛋白酶位点
pReceiver-WG02 T7 cell free N/A N-His Factor Xa
pReceiver-WG03 T7 cell free N/A N-HisSUMO SUMO Protease
pReceiver-WG04 T7 cell free N/A N-AviSUMO SUMO Protease
pReceiver-WG05 T7 cell free N/A N-HisAviSUMO SUMO Protease
pReceiver-WG09 T7 cell free N/A HisGST Tev protease
pReceiver-WG16 T7 cell free N/A N/A N/A
pReceiver-WG31 T7 cell free N/A N-HisSUMOAvi SUMO Protease
pReceiver-WG33 T7 cell free N/A N-TrxHisSUMO SUMO Protease

 

View available Anti-tag antibodies

点击了解更多ORF cDNA克隆信息

Download – OmicsLink™ ORF cDNA clone brochure

 

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