易锦生物提供的全部人、小鼠和大鼠ORF 克隆及其慢病毒、AAV 颗粒与重组蛋白均使用基于毛细管电泳的 Sanger 法“金标准”全长测序，保证氨基酸序列与NCBI 数据库一致。
All human and mice ORF clones and related Lentivirus, AAV particles and recombinant proteins provided by iGeneBio are fully sequenced using Sanger sequencing by capillary electrophoresis and Amino Acid Sequences are guaranteed to be matched with NCBI database.

慢病毒载体的ORF、启动子、shRNA、miRNA前体和miRNA抑制剂克隆

产品介绍

GeneCopoeia提供慢病毒载体的ORF、启动子、shRNA、miRNA 前体克隆和 miRNA抑制剂克隆，有丰富的融合标签供选择，例如IRES-eGFP、 Avitag®和HaloTag®等。生产过程有严格的质量控制，所有克隆经过全长测序，可用于即时表达。还提供优化的慢病毒包装质粒、细胞系、慢病毒浓缩试剂盒、慢病毒颗粒以及慢病毒包装服务。

慢病毒载体ORF、启动子、shRNA和microRNA克隆的优势

高效整合基因至多种靶细胞

慢病毒系统可高效地把基因转染到活体模式动物和几乎所有的哺乳动物细胞中，包括未分化、处于不活跃或停止生长的细胞，包括神经元细胞、原代细胞、干细胞。慢病毒转导效率接近100%。是理想的表达载体系统。

靶基因的高表达水平

慢病毒表达载体类型包括N或C末端带有融合标签、或不带标签。载体带有可进行高效率包装、转染并稳定整合进靶细胞的基因组中的序列元件，从而使得我们的表达克隆在靶细胞中高效表达。

慢病毒自身复制灭活

OmicsLink™人类ORF 慢病毒表达克隆所采用的载体是自身复制灭活的，由于细胞不带有病毒外壳基因，转染后的细胞并不能产生病毒粒子。此外整合进基因组后导致5’ LTR启动子失活，阻止了慢病毒的复制。

载体	启动子	宿主细胞	筛选标记	标签	蛋白酶位点
pReceiver-Lv117	CMV	Mammalian	Puromycin	N-3xHA	N/A
pReceiver-Lv118	CMV	Mammalian	Neomycin	N-3xHA	N/A
pReceiver-Lv145	EF1a	Mammalian	Hygromycin	SV40-hygromycin	N/A
pReceiver-Lv163	EF1a	Mammalian	Neomycin	SV40-neomycin	N/A
pReceiver-Lv170	CMV	Mammalian	Neomycin	C-Myc	N/A
pReceiver-Lv181	CMV	Mammalian	Puromycin	N-3xFlag	N/A
pReceiver-Lv185	PGK	Mammalian	Puromycin	Untagged	N/A
pReceiver-Lv188	CMV	Mammalian	N/A	C-eGFP	N/A
pReceiver-Lv193	CMV	Mammalian	Puromycin	C-eGFP(monomeric)	N/A
pReceiver-Lv195	EF1a	Mammalian	Puromycin	SV40-puromycin	N/A
pReceiver-Lv197	CMV	Mammalian	Blasticidin	Untagged	N/A
pReceiver-Lv218	CMV	Mammalian	Neomycin	IRES2-Neomycin	N/A
pReceiver-Lv219	CMV	Mammalian	Puromycin	IRES2-Puromycin	N/A
pReceiver-Lv221	CMV	Mammalian	N/A	IRES2-eYFP	N/A
pReceiver-Lv222	CMV	Mammalian	N/A	IRES2-eCFP	N/A
pReceiver-Lv233	EF1a	Mammalian	Puromycin	SV40-eGFP-IRES-Puromycin	N/A
pReceiver-Lv234	CAG	Mammalian	N/A	IRES2-eGFP	N/A
pReceiver-Lv235	CAG	Mammalian	Puromycin	SV40-eGFP-IRES-Puromycin	N/A
pReceiver-Lv236	CAG	Mammalian	Puromycin	Untagged	N/A
pReceiver-Lv237	CAG	Mammalian	Puromycin	C-Flag-SV40-Puromycin	N/A
pReceiver-Lv241	CMV	Mammalian	Puromycin	C-Flag-SV40-Puromycin	N/A
pReceiver-Lv243	CAG	Mammalian	Puromycin	C-Avi-Flag-SV40-Puromycin	N/A
pReceiver-Lv244	CMV	Mammalian	Hygromycin	SV40-mCherry-IRES-Hygromycin	N/A
pReceiver-Lv245	EF1a	Mammalian	Puromycin	cMyc-SV40-mCherry-IRES-Puromycin	N/A
pReceiver-Lv246	UBC	Mammalian	Puromycin	SV40-eGFP-IRES-Puromycin	N/A
pReceiver-Lv247	UBC	Mammalian	Puromycin	IRES2-mCherry-IRES-Puromycin	N/A
pReceiver-Lv253	EF1a	Mammalian	Puromycin	SV40-mcherry-IRES-Puromycin	N/A
pReceiver-Lv254	EF1a	Mammalian	Neomycin	SV40-mcherry-IRES-Neomycin	N/A
pReceiver-Lv105	CMV	Mammalian	Puromycin	Untagged	N/A
pReceiver-Lv151	CMV	Mammalian	Neomycin	Untagged	N/A
pReceiver-Lv152	CMV	Mammalian	Hygromycin	Untagged	N/A
pReceiver-Lv153	CMV	Mammalian	N/A	Untagged	N/A
pReceiver-Lv186	CMV	Mammalian	N/A	C-3xHA	N/A
pReceiver-Lv157	CMV	Mammalian	Neomycin	C-3xHA	N/A
pReceiver-Lv120	CMV	Mammalian	Puromycin	C-3xHA	N/A
pReceiver-Lv108	CMV	Mammalian	Puromycin	N-Avi	N/A
pReceiver-Lv124	CMV	Mammalian	Puromycin	C-Avi	N/A
pReceiver-Lv242	CMV	Mammalian	Puromycin	C-Avi-FLAG	N/A
pReceiver-Lv154	CMV	Mammalian	Neomycin	C-eCFP	N/A
pReceiver-Lv127	CMV	Mammalian	Puromycin	C-eCFP	N/A
pReceiver-Lv109	CMV	Mammalian	Puromycin	N-eCFP	N/A
pReceiver-Lv103	CMV	Mammalian	Puromycin	N-eGFP	N/A
pReceiver-Lv122	CMV	Mammalian	Puromycin	C-eGFP	N/A
pReceiver-Lv182	CMV	Mammalian	Neomycin	N-eGFP	N/A
pReceiver-Lv183	CMV	Mammalian	Neomycin	C-eGFP	N/A
pReceiver-Lv189	CMV	Mammalian	N/A	N-eGFP	N/A
pReceiver-Lv123	CMV	Mammalian	Puromycin	C-eYFP	N/A
pReceiver-Lv104	CMV	Mammalian	Puromycin	N-eYFP	N/A
pReceiver-Lv187	CMV	Mammalian	N/A	C-FLAG	N/A
pReceiver-Lv101	CMV	Mammalian	Neomycin	N-Flag	N/A
pReceiver-Lv102	CMV	Mammalian	Puromycin	N-Flag	N/A
pReceiver-Lv158	CMV	Mammalian	Neomycin	C-FLAG	N/A
pReceiver-Lv203	CMV	Mammalian	Puromycin	C-Flag-SV40-eGFP-IRES-puromycin	N/A
pReceiver-Lv216	CMV	Mammalian	Puromycin	C-Flag-SV40-mCherry-IRES-puromycin	N/A
pReceiver-Lv129	CMV	Mammalian	Puromycin	C-HaloTag	Tev
pReceiver-Lv110	CMV	Mammalian	Puromycin	N-HaloTag	Tev
pReceiver-Lv211	CMV	Mammalian	Hygromycin	C-HaloTag-IRES-hygromycin	N/A
pReceiver-Lv128	CMV	Mammalian	Puromycin	C-His	N/A
pReceiver-Lv215	CMV	Mammalian	N/A	IRES2-eGFP	N/A
pReceiver-Lv205	CMV	Mammalian	Puromycin	IRES2-eGFP-IRES-puromycin	N/A
pReceiver-Lv220	CMV	Mammalian	Hygromycin	IRES2-Hygromycin	N/A
pReceiver-Lv214	CMV	Mammalian	N/A	IRES2-mCherry	N/A
pReceiver-Lv213	CMV	Mammalian	Puromycin	IRES2-mCherry-IRES-puromycin	N/A
pReceiver-Lv111	CMV	Mammalian	Puromycin	N-mCherry	N/A
pReceiver-Lv130	CMV	Mammalian	Puromycin	C-mCherry	N/A
pReceiver-Lv155	CMV	Mammalian	Neomycin	C-mCherry	N/A
pReceiver-Lv107	CMV	Mammalian	Puromycin	N-Myc	N/A
pReceiver-Lv125	CMV	Mammalian	Puromycin	C-Myc	N/A
pReceiver-Lv126	CMV	Mammalian	Puromycin	C-Myc-His	N/A
pReceiver-Lv202	CMV	Mammalian	Puromycin	C-Myc-SV40-eGFP-IRES-puromycin	N/A
pReceiver-Lv204	CMV	Mammalian	Puromycin	C-Myc-SV40-mCherry-IRES-puromycin	N/A
pReceiver-Lv207	CMV	Mammalian	Hygromycin	SV40-eGFP-IRES-hygromycin	N/A
pReceiver-Lv201	CMV	Mammalian	Puromycin	SV40-eGFP-IRES-puromycin	N/A
pReceiver-Lv217	CMV	Mammalian	Puromycin	SV40-hLUC-IRES-puromycin	N/A
pReceiver-Lv206	CMV	Mammalian	Puromycin	SV40-mCherry-IRES-puromycin	N/A
pReceiver-Lv171	EF1a	Mammalian	N/A	IRES-eGFP	N/A
pReceiver-Lv173	EF1a	Mammalian	N/A	IRES-mcherry	N/A
pReceiver-Lv180	EF1a	Mammalian	Puromycin	IRES-mcherry-SV40-puromycin	N/A
pReceiver-Lv179	EF1a	Mammalian	Puromycin	IRES-eGFP-SV40-puromycin	N/A
pReceiver-Lv228	PGK	Mammalian	N/A	IRES2-eGFP	N/A
pReceiver-Lv229	PGK	Mammalian	N/A	IRES2-mcherry	N/A
pReceiver-Lv230	PGK	Mammalian	Puromycin	IRES2-mcherry-IRES-puromycin	N/A
pReceiver-Lv231	PGK	Mammalian	Puromycin	IRES2-eGFP-IRES-puromycin	N/A
pLX304	CMV	Mammalian	Blasticidin	C-V5	N/A

载体名称	启动子	筛选标记	报告基因	慢病毒骨架
psi-LVRH1GH	H1	Hygromycin	eGFP	HIV
psi-LVRH1GP	H1	Puromycin	eGFP	HIV
psi-LVRH1H	H1	Hygromycin	N/A	HIV
psi-LVRH1MH	H1	Hygromycin	mCherry	HIV
psi-LVRH1MP	H1	Puromycin	mCherry	HIV
psi-LVRH1P	H1	Puromycin	N/A	HIV
psi-LVRU6GH	U6	Hygromycin	eGFP	HIV
psi-LVRU6GP	U6	Puromycin	eGFP	HIV
psi-LVRU6H	U6	Hygromycin	N/A	HIV
psi-LVRU6MH	U6	Hygromycin	mCherry	HIV
psi-LVRU6MP	U6	Puromycin	mCherry	HIV
psi-LVRU6P	U6	Puromycin	N/A	HIV
psi-LVRInU6TGP	inducible U6	Puromycin	eGFP	HIV

载体名称	报告基因	筛选标记	启动子	慢病毒骨架
pEZX-MR02	mCherry	Puromycin	CMV	HIV
pEZX-MR03	eGFP	Puromycin	CMV	HIV

载体名称	报告基因	筛选标记	启动子	慢病毒骨架
pEZX-AM03	mCherry	Hygromycin	H1	HIV
pEZX-AM04	mCherry	Hygromycin	U6	HIV
pEZX-AM04p	mCherry	Puromycin	U6	HIV

载体名称	报告基因	示踪基因	筛选标记	载体骨架
pEZX-LvPG04	Gaussia luciferase (GLuc)	Secreted alkaline phosphatase (SEAP)	Puromycin	HIV
pEZX-LvPG02	Gaussia luciferase (GLuc)	N/A*	Puromycin	HIV
pEZX-LvPF02	eGFP	N/A*	Puromycin	HIV
pEZX-LvPM02	mCherry	N/A*	Puromycin	HIV
pEZX-LvPM03	tdTomato	N/A*	Puromycin	HIV

慢病毒载体的ORF、启动子、shRNA、miRNA前体和miRNA抑制剂克隆

慢病毒载体ORF、启动子、shRNA和microRNA克隆的优势

高效整合基因至多种靶细胞

靶基因的高表达水平

慢病毒自身复制灭活

ORF慢病毒表达载体(HIV)

shRNA慢病毒表达载体

microRNA前体慢病毒表达载体

microRNA抑制剂慢病毒表达载体

慢病毒载体的启动子报告克隆

Cas9 nuclease lentiviral expression clones

慢病毒载体的编码基因克隆

慢病毒载体的shRNA克隆

慢病毒载体的miRNA前体及inhibitor克隆

慢病毒载体的启动子克隆

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