– 高灵敏性海肾荧光素酶检测试剂盒
Luc-Pair™ Renilla Luciferase HS Assay Kit 是一款高灵敏性荧光素酶检测试剂盒，可高效、快速检测海肾（ Renilla luciferase ，RLuc）荧光素酶的活性。由于其具有极高的灵敏度， 可检测极低水平表达、调控和转染不佳的细胞的表达，也可搭配微孔板使用。适用于GeneCopoeia 及Promega的海肾荧光素表达载体。
产品特点
• 灵敏性高—本试剂盒对海肾荧光素酶产生的光信号极度敏感；
• 适用范围广—适用于多种不同的真核生物（ 脊椎动物、低等无脊椎动物）细胞系样品在微孔板（如：96孔板）或单管中检 测；
• 背景值低—背景更低，数据更准确；
• 操作简便—实验步骤简便；
• 重复性好—检测数据更可靠，且荧光素酶的浓度线性范围超过 9 个数量级。
同时，GeneCopoeia 还提供 Luc-Pair™ Duo-Luciferase Assay Kit 2.0 (高稳定性双荧光素酶检测试剂盒) 、 Luc-Pair™ Duo-Luciferase HS Assay Kit (高灵敏性双荧光素酶检测试剂盒)。
图1. 用GeneCopoeia 的 Luc-Pair™ 高灵敏性海肾荧光素酶检测试剂盒检测海肾荧光素酶的活性。用 Promega 的 pGL4.75 报告载体转染 HEK293 细胞，转染 48 小时后检测荧光信号，结果如上图所示。Ruc-GeneCopoeia代表 GeneCopoeia 的检测结果， FRuc-Promega代表 Promega 品牌试剂盒的检测结果。
图2. 用 GeneCopoeia 的 Luc-Pair™ 高灵敏性海肾荧光素酶检测试剂盒检测海肾荧光素酶信号的稳定性。用 Promega 的 pGL4.75 报告载体转染 HEK293 细胞，转染 48 小时后检测荧光信号，RLuc（RLuc-GeneCopoeia）的活性如上图所示。用自动加样器分配 RLuc 工作液，设置震动混合为 1 秒，然后立即检测，检测时间为 20 秒。
| 购买 | 货号 | 产品 | 描述 | 价格(￥) |
| ZX003 | Fluc + Rluc reporter clone | pEZX-FR03 包含萤火虫荧光素酶(Fluc)和海肾荧光素酶(Rluc)的报告载体 | 3960 | |
| ZX001 | Fluc + Rluc reporter clone | pEZX-FR01 包含萤火虫荧光素酶(Fluc)和海肾荧光素酶(Rluc)的报告载体 | 3960 | |
| ZX002 | Fluc + Rluc reporter clone | pEZX-FR02 包含萤火虫荧光素酶(Fluc)和海肾荧光素酶(Rluc)的报告载体 | 3960 |
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