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COVID-19 慢病毒 & AAV

GeneCopoeia有使用慢病毒和腺相关病毒(AAV)进行基因传递的全套优质的产品。

慢病毒的解决方法:

  • 慢病毒质粒​,可用于表达ORF cDNAs、基因启动子、miRNA前体和抑制剂、CRISPR sgRNA和 shRNA,第三代慢病毒包装。
  • 预制慢病毒,用于感染大多数哺乳动物细胞(包病毒包膜质粒为VSV-G基因),或用于感染表达ACE2细胞(包病毒包膜质粒为 SARS-CoV-2 S基因)。
  • 包装试剂盒, 用VSV-G基因作为包膜质粒的慢病毒包装试剂盒和用SARS-CoV-2 S作为包包质粒的慢病毒包装试剂盒

AAV解决方案:

  • 预制病毒, 用于多种血清型中许多哺乳动物细胞类型的感染。
  • 血清型检测包, 帮你确定哪种AAV血清型最适合感染你的目的细胞。

表达SARS-CoV-2病毒蛋白的慢病毒

用于表达SARS-CoV-2病毒蛋白的慢病毒颗粒有两个主要用途:

1、Lentifect™ SARS-CoV-2 S 蛋白假慢病毒,目前常用的慢病毒是由病毒包膜中表达囊泡状气孔病毒G糖蛋白(VSV-G)产生的,这类慢病毒能够广泛进入哺乳动物细胞。然而在慢病毒包装过程中,编码VSV-G的包装质粒可以被表达SARS-CoV-2 S 蛋白的质粒所替代,这类病毒针对性的感染表达ACE2的细胞,而ACE2是人类SARS-CoV-2感染受体 (Ou, X., et al., 2020. Nature Communications doi: 10.1038/s41467-020-15562-9; Hoffman, M., et al., 2020. Cell doi: 10.1016/j.cell.2020.02.052)。这些病毒可用于疫苗开发和阻止SARS-CoV-2进入细胞的抗体测试。如果你想订购带有SARS-CoV-2 S蛋白的假慢病毒颗粒,请联系我们。

2、Expression of SARS-CoV-2 病毒蛋白慢病毒(VSV-G​标准型),我们有80多种慢病毒载体类型,要订购表达SARS-CoV-2病毒蛋白的高滴度、即用慢病毒颗粒,请从下表中选择产品ID。

Product ID Protein accession Symbol Alias Description
CoV201 YP_009724389.1 orf1ab GU280_gp01 orf1ab polyprotein [Severe acute respiratory syndrome coronavirus 2}, full-length gene
CoV202 YP_009725297.1 orf1ab GU280_gp01 leader protein [Severe acute respiratory syndrome coronavirus 2], mature peptide*
CoV203 YP_009725298.1 orf1ab GU280_gp01 nsp2n [Severe acute respiratory syndrome coronavirus 2], mature peptide*
CoV204 YP_009725299.1 orf1ab GU280_gp01 nsp3n [Severeacute respiratory syndrome coronavirus 2], mature peptide*
CoV205 YP_009725300.1 orf1ab GU280_gp01 nsp4n [Severe acute respiratory syndrome coronavirus 2], mature peptide*
CoV206 YP_009725301.1 orf1ab GU280_gp01 3C-like proteinasen [Severe acute respiratory syndrome coronavirus 2], mature peptide*
CoV207 YP_009725302.1 orf1ab GU280_gp01 nsp6n [Severe acute respiratory syndrome coronavirus 2], mature peptide*
CoV208 YP_009725303.1 orf1ab GU280_gp01 nsp7n [Severe acute respiratory syndrome coronavirus 2], mature peptide*
CoV209 YP_009725304.1 orf1ab GU280_gp01 nsp8n [Severe acute respiratory syndrome coronavirus 2], mature peptide*
CoV210 YP_009725305.1 orf1ab GU280_gp01 nsp9n [Severe acute respiratory syndrome coronavirus 2], mature peptide*
CoV211 YP_009725306.1 orf1ab GU280_gp01 nsp10n [Severe acute respiratory syndrome coronavirus 2], mature peptide*
CoV212 YP_009725307.1 orf1ab GU280_gp01 RNA-dependent RNA polymerasen [Severe acute respiratory syndrome coronavirus 2], mature peptide*
CoV213 YP_009725308.1 orf1ab GU280_gp01 helicasen [Severe acute respiratory syndrome coronavirus 2], mature peptide*
CoV214 YP_009725309.1 orf1ab GU280_gp01 3'-to-5' exonucleasen [Severe acute respiratory syndrome coronavirus 2], mature peptide*
CoV215 YP_009725310.1 orf1ab GU280_gp01 endoRNAsen [Severe acute respiratory syndrome coronavirus 2], mature peptide*
CoV216 YP_009725311.1 orf1ab GU280_gp01 2'-O-ribose methyltransferasen [Severe acute respiratory syndrome coronavirus 2], mature peptide*
CoV217 YP_009725295.1 orf1a GU280_gp01 orf1a polyproteinn [Severe acute respiratory syndrome coronavirus 2], full-length gene
CoV218 YP_009725312.1 orf1a GU280_gp01 produced by pp1a onlyn [Severe acute respiratory syndrome coronavirus 2], mature peptide*
CoV219 YP_009724390.1 S GU280_gp02 surface glycoprotein;structural protein; spike proteinn [Severe acute respiratory syndrome coronavirus 2], full-length gene
CoV220 YP_009724390.1 S1 GU280_gp02 surface glycoprotein subunit 1 [Severe acute respiratory syndrome coronavirus 2], mature peptide
CoV221 YP_009724390.1 S2 GU280_gp02 surface glycoprotein subunit 2 [Severe acute respiratory syndrome coronavirus 2], mature peptide*
CoV222 YP_009724390.1 RBD GU280_gp02 Receptor binding domain [Severe acute respiratory syndrome coronavirus 2], mature peptide*
CoV223 YP_009724391.1 ORF3a GU280_gp03 ORF3a protein [Severe acute respiratory syndrome coronavirus 2], full-length gene
CoV224 YP_009724392.1 E GU280_gp04 envelope protein [Severe acute respiratory syndrome coronavirus 2], full-length gene
CoV225 YP_009724393.1 M GU280_gp05 membrane glycoprotein [Severe acute respiratory syndrome coronavirus 2], full-length gene
CoV226 YP_009724394.1 ORF6 GU280_gp06 ORF6 protein [Severe acute respiratory syndrome coronavirus 2], full-length gene
CoV227 YP_009724395.1 ORF7a GU280_gp07 ORF7a protein [Severe acute respiratory syndrome coronavirus 2], full-length gene
CoV228 YP_009725296.1 ORF7b GU280_gp08 ORF7b [Severe acute respiratory syndrome coronavirus 2], full-length gene
CoV229 YP_009724396.1 ORF8 GU280_gp09 ORF8 protein [Severe acute respiratory syndrome coronavirus 2], full-length gene
CoV230 YP_009724397.2 N GU280_gp10 nucleocapsid phosphoprotein [Severe acute respiratory syndrome coronavirus 2], full-length gene
CoV231 YP_009725255.1 ORF10 GU280_gp11 ORF10 protein [Severe acute respiratory syndrome coronavirus 2], full-length gene

*注:成熟肽是前体蛋白的加工而成。为了使这些蛋白在细胞中表达,在ORF序列的5'端添加了ATG。

表达SARS-CoV-2宿主蛋白的慢病毒

    预制表达ACE2慢病毒

    订购 货号 规格 滴度 启动子 筛选标记 标记 载体 价格
    LPP-U1285-Lv152-100 100 μl >108 TU/mL纯化 CMV
    Hygromycin
    无标记 pReceiver-Lv152 2000

     

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    定制表达ACE2的慢病毒

    • 可为你提供80多种载体类型的慢病毒颗粒来选择表达ACE2。

    定制表达TMPRSS2的慢病毒

    • 可提供80多种载体类型的慢病毒颗粒来选择表达TMPRSS2

     

      AAV

      可提供80多种载体类型的的AAV颗粒来选择表达目的基因。

      • 定制表达ACE2 的AAV颗粒 
      • 定制表达TMPRSS2 的AAV颗粒
      • 定制表达SARS-CoV-2病毒蛋白的AAV颗粒
       

        我们也提供其它冠状病毒(SARS-CoV、MERS-CoV)蛋白的慢病毒和AAV解决方案。