Monthly Archives: 三月 2015

MycoGuard™ 支原体检测试剂盒(生物化学发光法)

产品介绍 订购 相关产品 产品介绍 MycoGuard™支原体检测试剂盒(生物化学发光法)   支原体污染是细胞传代及培养中的常见问题。由于自身缺乏生物合成功能,支原体通常依靠与细胞竞争培养基中的养分存活,易导致细胞增殖速度减慢,基因表达、代谢特征及细胞形态发生变化,影响实验结果的准确性。 传统的支原体检测方法需耗费大量时间,且无法在早期发现污染。MycoGuard™检测试剂盒可快速、简单检测出细胞培养过程中存在的支原体。利用支原体酶与MycoGuard™底物反应催化产生ATP,且ATP浓度与其激发的荧光强度成线性关系的原理,可通过生物发光的方式检测样品中的支原体。 为防范您的细胞被支原体污染,需要......
Posted in 产品, 试剂与试剂盒 Tagged

Genome editing products

基因组编辑产品   常见问题 我能用基因组编辑技术在基因组上引入一个点突变吗? 能:能。对于这种基因组编辑操作,您需要带有靶点同源重组臂以及所需点突变的供体 DNA。供体 DNA 可以是一个质粒或一条单链的寡聚核苷酸。供体 DNA 需要与基因组编辑工具(TALEN 或 CRISPR)进行共转染。基因组编辑工具在靶点生成的 DNA 双链断裂(DSB),细胞在存在供体 DNA 的情况下,会将供体 DNA 作为修复模板,利用同源重组(HR)机制修复 DSB。  易锦提供供体质粒设计及构建服务。我们能构建带有目的修饰及靶序列同源重组臂的供体质粒。质粒同时会敲入筛选标记(如嘌呤霉素抗性基因)或 copGFP 报告基因。这些筛选标......
Posted in 常见问题(FAQs)

Biotin-11-UTP, lyophilized powder

Introduction To order Related products Introduction Biotin-11-UTP (cat. no. C404B) is supplied in lyophilized form. Biotin-11-UTP can be enzymatically incorporated into RNA with T7, T3 and SP6 RNA polymerases , as well as other RNA modifying enzymes. The number‘11’ is the number of atoms in the linker between biotin and UTP. Biotinylated RNA can be used in place of radioactively labeled RNA in many applications, including cRNA amplification and labeling for microarray target preparation. Detection is then carried out via one of a variety of streptavidin-based methods. ......
Posted in Labeled Nucleotides, 产品, 荧光标记和检测技术 Tagged

Labeled Nucleotides

Introduction To order Related products Introduction Applied BioProbes offers 5′-aminoallyl dUTP and UTP analogs, as well as conjugates with biotin and various fluorescent dyes, featuring our exceptionally bright and photostable Andy Fluor dyes. Fluorescent and biotin-labeled dUTP and UTP are widely used for enzymatic incorporation into DNA or RNA. Advantages Bright signals with low background Wide variety of fluorophores. Great for many applications, like Fluorescence in situ Hybridization (FISH; Figure 1), TUNEL (Figure 2), and more.   Figure 1. Fluore......
Posted in 产品, 荧光标记和检测技术 Tagged