技术

预制稳转株（Stable cell lines）

< 2018 Sokhi, U.K., et al. (2018). Dissection and function of autoimmunity-associated TNFAIP3 (A20) gene enhancers in humanized mouse models. Nature Communications. DOI: 10.1038/s41467-018-03081-7. [H...

转染试剂（Transfection reagents）

< 2018 Zhang, Y., et al. (2018). microRNA-874 suppresses tumor proliferation and metastasis in hepatocellular carcinoma by targeting the DOR/EGFR/ERK pathway. Cell Death and Disease. DOI: 10.1038/s41419-0...

荧光素酶检测（Luciferase Assays）

< 2018 Xiong, K., et al. (2018). MicroRNA-9 functions as a tumor suppressor and enhances radio-sensitivity in radio-resistant A549 cells by targeting neuropilin 1. Oncology Letters. DOI: 10.3892/ol.2017.7705. [...

重组蛋白

Livesey, K. et al. (2012) p53/HMGB1 complexes regulate autophagy and apoptosis. Cancer Res April 15, 2012 72; 1996. .

启动子报告克隆（Promoter Reporter Clones）

Celardo, I. et al. (2013) Caspase-1 is a novel target of p63 in tumor suppression. Cell Death and Disease (2013) 4, e645; doi:10.1038/cddis.2013.175. . Zheng, H. et al. (2013) Glycogen synthase kinase-3 beta regulates Snai...

qPCR Arrays

Huang, L. et al. (2012) Downregulation of Six MicroRNAs Is Associated with Advanced Stage, Lymph Node Metastasis and Poor Prognosis in Small Cell Carcinoma of the Cervix. PLoS ONE 7(3): e33762. doi:10.1371/journal.pone.0033762....

Gateway克隆技术原理

Gateway克隆技术基于已研究的非常清楚的λ噬菌体位点特异重组系统，使用Int (integrase)和 IHF (Integration Host Factor) 催化重组反应， attB和attP位点的重组反应会生成attL和attR...

Gateway® Technology

Gateway®克隆技术的基础是 λ噬菌体的位点特异性重组反应，一种保存遗传信息的有效的生化途径（见图1）。与传统的需要DNA限制性内切酶、DNA连接酶、凝胶电泳和DNA片段纯化等多个步骤的繁琐亚克隆方法相比，该方法只需一步生化反...

OmicsLink™ 克隆的应用及与全长转录本cDNA克隆的区别

可以在E.coli 细胞中表达蛋白可以通过反向转染在哺乳动物细胞中表达蛋白可以在体外无细胞体系中表达蛋白表达带有标签的蛋白，可用于纯化和下游分析产生原位杂...

qPCR验证引物与试剂盒

Peng, J. et al (2013) Expression Patterns of miR-124, miR-134, miR-132, and miR-21 in an Immature Rat Model and Children with Mesial Temporal Lobe Epilepsy. Journal of Molecular Neuroscience, onli...

构建目的表达克隆的高效方法

ORFEXPRESS™-Shuttle Clone与目的载体attL和attR位点的重组反应可以生成目的表达克隆。这种特异性极高的重组反应不会导致LXR位点间碱基的增加或删除。在 ORFEXPRESS™-Shuttle Clone中，起始...

慢病毒产品与服务

2017 Teng, Y., et al. (2017). MVP-mediated exosomal sorting of miR-193a promotes colon cancer progression. Nature Communications doi: 10.1038/ncomms14448 [Lentiviral particles expressing sgRNA targeting MVP] ...

ORF/cDNA克隆

< 2018 Ramani, K., et al. (2018). Role of A-Kinase Anchoring Protein Phosphorylation in Alcohol-Induced Liver Injury and Hepatic Stellate Cell Activation. The American Journal. of Path...

分子生物学试剂

< 2016 Bid, HK., et al. (2016). The Bromodomain BET inhibitor JQ1 Suppresses Tumor Angiogenesis in Models of Childhood Sarcoma. Molecular Cancer Therapeutics,Published ...

基因下调—shRNA

< 2018 Yang, G., et al. (2018). The TET2/E-cadherin/β-catenin regulatory loop confers growth and invasion in hepatocellular carcinoma cells. Experimental Cell Research DOI: 10.1016/j.yexcr.2018.01.011 [OMICSLIN...

miRNA解决方案

Peng, J. et al (2013) Expression Patterns of miR-124, miR-134, miR-132, and miR-21 in an Immature Rat Model and Children with Mesial Temporal Lobe Epilepsy. Journal of Molecular Neuroscience, onli...