技术

抗体（Antibodies）

<2017 Yang, B., et al. (2017). Altered expression of BDNF, BDNF pro-peptide and their precursor proBDNF in brain and liver tissues from psychiatric disorders: rethinking the brain–liver axis. Translational Psy...

基因组编辑工具（CRISPR & TALEN）

>2019 Gürtler, S., et al. (2019). Tafazzin-dependent cardiolipin composition in C6 glioma cells correlates with changes in mitochondrial and cellular functions, and cellular proliferation. Biochimica et Biophysica A...

荧光标记和检测技术（Fluorescent Labeling and Detection）

>2019 Ding, Q., et al. (2019). The role of the apoptosis-related protein BCL-B in the regulation of mitophagy in hepatic stellate cells during the regression of liver fibrosis. Experimental and Molecular Medicine doi: 10...

预制稳转株（Stable cell lines）

< 2018 Sokhi, U.K., et al. (2018). Dissection and function of autoimmunity-associated TNFAIP3 (A20) gene enhancers in humanized mouse models. Nature Communications. DOI: 10.1038/s41467-018-03081-7. [H...

转染试剂（Transfection reagents）

>2019 Lakshmipathi, J., et al. (2019). Identification of NFAT5 as a transcriptional regulator of the EDN1gene in collecting duct. American Journal of Renal Physiology doi: 10.1152/ajprenal.00509.2018 [EndoFectin™ M...

荧光素酶检测（Luciferase Assays）

>2019 Jiang, Q., et al. (2019). Hyper-Editing of Cell-Cycle Regulatory and Tumor Suppressor RNA Promotes Malignant Progenitor Propagation. Cancer Cell doi: 10.1016/j.ccell.2018.11.017 [Secrete-Pair™ Dual Luminescen...

重组蛋白

<2016 Zhou, Y., et al. (2016) Competitive profiling of celastrol targets in human cervical cancer HeLa cells via quantitative chemical proteomics . Molecular Biosystems 10.1039/C6MB00691D [Recombinant human GSTO1 pr...

启动子报告克隆（Promoter Reporter Clones）

< 2019 Wang, H-B., et al. (2019). Down-regulated expression of LINC00518 prevents epithelial cell growth and metastasis in breast cancer through the inhibition of CDX2 methylation and activation of the Wnt signaling path...

qPCR Arrays

Huang, L. et al. (2012) Downregulation of Six MicroRNAs Is Associated with Advanced Stage, Lymph Node Metastasis and Poor Prognosis in Small Cell Carcinoma of the Cervix. PLoS ONE 7(3): e33762. doi:10.1371/journal.pone.0033762....

Gateway克隆技术原理

Gateway克隆技术基于已研究的非常清楚的λ噬菌体位点特异重组系统，使用Int (integrase)和 IHF (Integration Host Factor) 催化重组反应， attB和attP位点的重组反应会生成attL和attR...

Gateway® Technology

Gateway®克隆技术的基础是 λ噬菌体的位点特异性重组反应，一种保存遗传信息的有效的生化途径（见图1）。与传统的需要DNA限制性内切酶、DNA连接酶、凝胶电泳和DNA片段纯化等多个步骤的繁琐亚克隆方法相比，该方法只需一步生化反...

OmicsLink™ 克隆的应用及与全长转录本cDNA克隆的区别

可以在E.coli 细胞中表达蛋白可以通过反向转染在哺乳动物细胞中表达蛋白可以在体外无细胞体系中表达蛋白表达带有标签的蛋白，可用于纯化和下游分析产生原位杂...

qPCR验证引物与试剂盒

<2019 Yao, X., et al. (2019). Interleukin 4 inhibits high mobility group box-1 protein-mediated NLRP3 inflammasome formation by activating peroxisome proliferator-activated receptor-γ in astrocytes. Biochemical and...

构建目的表达克隆的高效方法

ORFEXPRESS™-Shuttle Clone与目的载体attL和attR位点的重组反应可以生成目的表达克隆。这种特异性极高的重组反应不会导致LXR位点间碱基的增加或删除。在 ORFEXPRESS™-Shuttle Clone中，起始...

慢病毒产品与服务

>2019 Al-Khadairi, G., et al. (2019). PRAME promotes epithelial-to-mesenchymal transition in triple negative breast cancer. Journal of Translational Medicine doi: 10.1186/s12967-018-1757-3 [Lentifect™ Lentiviral pa...

ORF/cDNA克隆

< 2019 Wang, J., et al. (2019). Fibrinogen-like Protein 1 Is a Major Immune Inhibitory Ligand of LAG-3. Cell. 2019 Jan 10;176(1-2):334-347.e12. doi: 10.1016/j.cell.2018.11.010. [>60...

分子生物学试剂

< 2016 Bid, HK., et al. (2016). The Bromodomain BET inhibitor JQ1 Suppresses Tumor Angiogenesis in Models of Childhood Sarcoma. Molecular Cancer Therapeutics,Published ...

基因下调—shRNA

< 2019 Song, N., et al. (2019). Knockdown of high mobility group box 3 impairs cell viability and colony formation but increases apoptosis in A549 human non‑small cell lung cancer cells. Oncology eports doi: 10.3892/ol.2...

miRNA解决方案

<2019 Jiang, Q., et al. (2019). Hyper-Editing of Cell-Cycle Regulatory and Tumor Suppressor RNA Promotes Malignant Progenitor Propagation. Cancer Cell doi: 10.1016/j.ccell.2018.11.017 [miTarget™ MDM2 3' UTR clo...