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慢病毒载体的ORF、启动子、shRNA、miRNA前体和miRNA抑制剂克隆

产品介绍

GeneCopoeia提供慢病毒载体的ORF启动子shRNAmiRNA前体克隆miRNA抑制剂克隆,有丰富的融合标签供选择,例如IRES-eGFP、 Avitag®和HaloTag®等。生产过程有严格的质量控制,所有克隆经过全长测序,可用于即时表达。还提供优化的慢病毒包装质粒、细胞系、慢病毒浓缩试剂盒、 慢病毒颗粒以及慢病毒包装服务

 

慢病毒载体ORF、启动子、shRNA和microRNA克隆的优势

高效整合基因至多种靶细胞

慢病毒系统可高效地把基因转染到活体模式动物和几乎所有的哺乳动物细胞中,包括未分化、处于不活跃或停止生长的细胞,包括神经元细胞、原代细胞、干细胞。慢病毒转导效率接近100%。是理想的表达载体系统。

 

靶基因的高表达水平

慢病毒表达载体类型包括N或C末端带有融合标签、或不带标签。载体带有可进行高效率包装、转染并稳定整合进靶细胞的基因组中的序列元件,从而使得我们的表达克隆在靶细胞中高效表达。

 

慢病毒自身复制灭活

OmicsLink™人类ORF 慢病毒表达克隆所采用的载体是自身复制灭活的,由于细胞不带有病毒外壳基因,转染后的细胞并不能产生病毒粒子。此外整合进基因组后导致5’ LTR启动子失活,阻止了慢病毒的复制。

 

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慢病毒载体

浏览 ORF/cDNA慢病毒载体, shRNA慢病毒载体, microRNA慢病毒载体启动子慢病毒载体

 

ORF慢病毒表达载体(HIV)

 

 

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载体 启动子 宿主细胞 筛选标记 标签 蛋白酶位点
pReceiver-Lv115 EF1a Lentiviral Hygromycin Untagged N/A
pReceiver-Lv117 CMV Lentiviral Puromycin N-3xHA N/A
pReceiver-Lv118 CMV Lentiviral Neomycin N-3xHA N/A
pReceiver-Lv160 EF1a Lentiviral Neomycin Untagged N/A
pReceiver-Lv170 CMV Lentiviral Neomycin C-Myc N/A
pReceiver-Lv181 CMV Lentiviral Puromycin N-3xFlag N/A
pReceiver-Lv185 PGK Lentiviral Puromycin Untagged N/A
pReceiver-Lv188 CMV Lentiviral N/A C-eGFP N/A
pReceiver-Lv193 CMV Lentiviral Puromycin C-eGFP(monomeric) N/A
pReceiver-Lv197 CMV Lentiviral Blasticidin Untagged N/A
pReceiver-Lv218 CMV Lentiviral Neomycin IRES2-Neomycin N/A
pReceiver-Lv219 CMV Lentiviral Puromycin IRES2-Puromycin N/A
pReceiver-Lv221 CMV Lentiviral N/A IRES2-eYFP N/A
pReceiver-Lv222 CMV Lentiviral N/A IRES2-eCFP N/A
pReceiver-Lv233 EF1a Lentiviral Puromycin SV40-eGFP-IRES-Puromycin N/A
pReceiver-Lv234 CAG Lentiviral N/A IRES2-eGFP N/A
pReceiver-Lv235 CAG Lentiviral Puromycin SV40-eGFP-IRES-Puromycin N/A
pReceiver-Lv236 CAG Lentiviral Puromycin Untagged N/A
pReceiver-Lv237 CAG Lentiviral Puromycin C-Flag-SV40-Puromycin N/A
pReceiver-Lv241 CMV Lentiviral Puromycin C-Flag-SV40-Puromycin N/A
pReceiver-Lv243 CAG Lentiviral Puromycin C-Avi-Flag-SV40-Puromycin N/A
pReceiver-Lv244 CMV Lentiviral Hygromycin SV40-mCherry-IRES-Hygromycin N/A
pReceiver-Lv245 EF1a Lentiviral Puromycin cMyc-SV40-mCherry-IRES-Puromycin N/A
pReceiver-Lv246 UBC Lentiviral Puromycin SV40-eGFP-IRES-Puromycin N/A
pReceiver-Lv247 UBC Lentiviral Puromycin IRES2-mCherry-IRES-Puromycin N/A
pReceiver-Lv105 CMV Lentiviral Puromycin Untagged N/A
pReceiver-Lv151 CMV Lentiviral Neomycin Untagged N/A
pReceiver-Lv152 CMV Lentiviral Hygromycin Untagged N/A
pReceiver-Lv153 CMV Lentiviral N/A Untagged N/A
pReceiver-Lv156 EF1a Lentiviral Puromycin Untagged N/A
pReceiver-Lv186 CMV Lentiviral N/A C-3xHA N/A
pReceiver-Lv157 CMV Lentiviral Neomycin C-3xHA N/A
pReceiver-Lv120 CMV Lentiviral Puromycin C-3xHA N/A
pReceiver-Lv108 CMV Lentiviral Puromycin N-Avi N/A
pReceiver-Lv124 CMV Lentiviral Puromycin C-Avi N/A
pReceiver-Lv242 CMV Lentiviral Puromycin C-Avi-FLAG N/A
pReceiver-Lv154 CMV Lentiviral Neomycin C-eCFP N/A
pReceiver-Lv127 CMV Lentiviral Puromycin C-eCFP N/A
pReceiver-Lv109 CMV Lentiviral Puromycin N-eCFP N/A
pReceiver-Lv103 CMV Lentiviral Puromycin N-eGFP N/A
pReceiver-Lv122 CMV Lentiviral Puromycin C-eGFP N/A
pReceiver-Lv182 CMV Lentiviral Neomycin N-eGFP N/A
pReceiver-Lv183 CMV Lentiviral Neomycin C-eGFP N/A
pReceiver-Lv189 CMV Lentiviral N/A N-eGFP N/A
pReceiver-Lv123 CMV Lentiviral Puromycin C-eYFP N/A
pReceiver-Lv104 CMV Lentiviral Puromycin N-eYFP N/A
pReceiver-Lv187 CMV Lentiviral N/A C-FLAG N/A
pReceiver-Lv101 CMV Lentiviral Neomycin N-Flag N/A
pReceiver-Lv102 CMV Lentiviral Puromycin N-Flag N/A
pReceiver-Lv158 CMV Lentiviral Neomycin C-FLAG N/A
pReceiver-Lv203 CMV Lentiviral Puromycin C-Flag-SV40-eGFP-IRES-puromycin N/A
pReceiver-Lv216 CMV Lentiviral Puromycin C-Flag-SV40-mCherry-IRES-puromycin N/A
pReceiver-Lv129 CMV Lentiviral Puromycin C-HaloTag Tev
pReceiver-Lv110 CMV Lentiviral Puromycin N-HaloTag Tev
pReceiver-Lv211 CMV Lentiviral Hygromycin C-HaloTag-IRES-hygromycin N/A
pReceiver-Lv128 CMV Lentiviral Puromycin C-His N/A
pReceiver-Lv215 CMV Lentiviral N/A IRES2-eGFP N/A
pReceiver-Lv205 CMV Lentiviral Puromycin IRES2-eGFP-IRES-puromycin N/A
pReceiver-Lv220 CMV Lentiviral Hygromycin IRES2-Hygromycin N/A
pReceiver-Lv214 CMV Lentiviral N/A IRES2-mCherry N/A
pReceiver-Lv213 CMV Lentiviral Puromycin IRES2-mCherry-IRES-puromycin N/A
pReceiver-Lv111 CMV Lentiviral Puromycin N-mCherry N/A
pReceiver-Lv130 CMV Lentiviral Puromycin C-mCherry N/A
pReceiver-Lv155 CMV Lentiviral Neomycin C-mCherry N/A
pReceiver-Lv107 CMV Lentiviral Puromycin N-Myc N/A
pReceiver-Lv125 CMV Lentiviral Puromycin C-Myc N/A
pReceiver-Lv126 CMV Lentiviral Puromycin C-Myc-His N/A
pReceiver-Lv202 CMV Lentiviral Puromycin C-Myc-SV40-eGFP-IRES-puromycin N/A
pReceiver-Lv204 CMV Lentiviral Puromycin C-Myc-SV40-mCherry-IRES-puromycin N/A
pReceiver-Lv207 CMV Lentiviral Hygromycin SV40-eGFP-IRES-hygromycin N/A
pReceiver-Lv201 CMV Lentiviral Puromycin SV40-eGFP-IRES-puromycin N/A
pReceiver-Lv217 CMV Lentiviral Puromycin SV40-hLUC-IRES-puromycin N/A
pReceiver-Lv206 CMV Lentiviral Puromycin SV40-mCherry-IRES-puromycin N/A
pReceiver-Lv165 EF1a Lentiviral N/A IRES2-eGFP N/A
pReceiver-Lv166 EF1a Lentiviral N/A IRES2-mcherry N/A
pReceiver-Lv224 EF1a Lentiviral Puromycin IRES2-mcherry-IRES-puromycin N/A
pReceiver-Lv225 EF1a Lentiviral Puromycin IRES2-eGFP-IRES-puromycin N/A
pReceiver-Lv228 PGK Lentiviral N/A IRES2-eGFP N/A
pReceiver-Lv229 PGK Lentiviral N/A IRES2-mcherry N/A
pReceiver-Lv230 PGK Lentiviral Puromycin IRES2-mcherry-IRES-puromycin N/A
pReceiver-Lv231 PGK Lentiviral Puromycin IRES2-eGFP-IRES-puromycin N/A
pLX304 CMV Lentiviral (ORFeome) Blasticidin C-V5 N/A
 
   

 

shRNA慢病毒表达载体

 

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载体名称 启动子 筛选标记 报告基因 慢病毒骨架
psi-LVRH1GH H1 Hygromycin eGFP HIV
psi-LVRH1GP H1 Puromycin eGFP HIV
psi-LVRH1H H1 Hygromycin N/A HIV
psi-LVRH1MH H1 Hygromycin mCherry HIV
psi-LVRH1MP H1 Puromycin mCherry HIV
psi-LVRH1P H1 Puromycin N/A HIV
psi-LVRU6GH U6 Hygromycin eGFP HIV
psi-LVRU6GP U6 Puromycin eGFP HIV
psi-LVRU6H U6 Hygromycin N/A HIV
psi-LVRU6MH U6 Hygromycin mCherry HIV
psi-LVRU6MP U6 Puromycin mCherry HIV
psi-LVRU6P U6 Puromycin N/A HIV
psi-LVRInU6TGP inducible U6 Puromycin eGFP HIV

 

   

 

microRNA前体慢病毒表达载体

 

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载体名称 报告基因 筛选标记 启动子 慢病毒骨架
pEZX-MR02 mCherry Puromycin CMV HIV
pEZX-MR03 eGFP Puromycin CMV HIV

 

   

 

microRNA抑制剂慢病毒表达载体

 

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载体名称 报告基因 筛选标记 启动子 慢病毒骨架
pEZX-AM03 mCherry Hygromycin H1 HIV
pEZX-AM04 mCherry Hygromycin U6 HIV
pEZX-AM04p mCherry Puromycin U6 HIV

 

 

慢病毒载体的启动子报告克隆

 

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载体名称 报告基因 示踪基因 筛选标记 载体骨架
pEZX-LvPG04 Gaussia luciferase (GLuc) Secreted alkaline phosphatase (SEAP) Puromycin HIV
pEZX-LvPG02 Gaussia luciferase (GLuc) N/A* Puromycin HIV
pEZX-LvPF02 eGFP N/A* Puromycin HIV
pEZX-LvPM02 mCherry N/A* Puromycin HIV
pEZX-LvPM03 tdTomato N/A* Puromycin HIV

 

Cas9 nuclease lentiviral expression clones

载体名称 启动子 报告基因 筛选标记 载体骨架
CP-LvC9NU-01 CMV N/A Neomycin HIV
CP-LvC9NU-02 CMV eGFP Neomycin HIV
CP-LvC9NU-08 EF1α N/A Puromycin HIV
CP-LvC9NU-09 EF1α eGFP Neomycin HIV
CP-LvC9NU-10 EF1α eGFP Hygromycin HIV

 

订购

选择您需要的人或小鼠慢病毒骨架预制克隆。

 

慢病毒载体的编码基因克隆

 

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慢病毒载体的shRNA克隆

 

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慢病毒载体的miRNA前体及inhibitor克隆

 

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慢病毒载体的启动子克隆

 

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 Plasmid Prep Protocol for Lentiviral clones

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