– 高灵敏性双荧光素酶检测试剂盒
Luc-Pair™ Duo-Luciferase HS Assay Kit 是一款高灵敏性双荧光素酶检测试剂盒，可高效、快速检测萤火虫（ Firefly luciferase ，FLuc）和海肾（Renilla luciferase ，Rluc）荧光素酶的活性。由于其具有极高的灵敏度， 可检测极低水平表达、调控和转染不佳的细胞的表达，也可搭配微孔板使用。适用于 GeneCopoeia 及 Promega 的萤火虫和海肾荧光素表达载体。本试剂盒是用于检测miRNA对靶基因抑制效率的理想工具。
产品特点
• 灵敏性高—本试剂盒对萤火虫和海肾荧光素酶产生的光信号极度敏感（见图1）；
• 适用范围广—适用于多种不同的真核生物（脊椎动物、低等无脊椎动物）细胞系样品在微孔板（如 96孔板）或 单管中检测；
• 背景值低—背景更低，数据更准确；
• 操作简便—海肾荧光素酶反应液包含了萤火虫荧光素酶的淬灭剂（见图2）；终止萤火虫荧光素酶发光，同时启动海肾荧 光素酶反应，只需2步实验；
• 重复性好—检测数据更可靠，且荧光素酶的浓度线性范围超过 9 个数量级；
• 兼容性强—与 Promega 的萤火虫和海肾荧光素表达载体相兼容。
图1. 用 GeneCopoeia 的 Luc-Pair™ 高灵敏性双荧光素酶检测试剂盒检测萤火虫和海肾荧光素酶的活性。用 Promega 的 pGL4.13 和 pGL4.75 报告载体转染 HEK293 细胞，转染 48 小时后分别检测荧光信号，效果如上图所示。Fluc-GeneCopoeia 和 Rluc-GeneCopoeia 代表 GeneCopoeia 的检测结果，Fluc-Promega 和 Rluc-Promega 代表 Promega 品牌试剂盒的检测结果。
图2. 萤火虫荧光素酶的活性被 Luc-H Buffer II 淬灭。用 Promega 的 pGL4.13 和 pGL4.75 报告载体转染 HEK293 细胞，转染 48 小时后先检测萤火虫荧光信号（左柱）， 然后把 1 × Luc-H Buffer II【不包含（中柱）或包含（右柱）底物】加入到微孔板后，用光度计再测荧光值。实验结果表明约 99.9% 的萤火虫荧光素酶活性被淬灭（中柱）， 而海肾荧光素酶反应不受影响（右柱）。
载体选择
GeneCopoeia 提供萤火虫荧光素酶（Firefly luciferase）报告载体，可供广大研究者自由操作；只需将目的片段插入报告载体，即可应用荧光素酶检测试剂。 点击载体名称可查看载体图谱信息。
| 货号 | 载体 | 启动子 | 报告基因 | 示踪基因 | 筛选标记 | 抗性 | 载体类型 |
| ZX001 | pEZX-FR01 | N/A* | Firefly luciferase (hLuc) | Renilla luciferase (Rluc) | Puromycin | Kanamycin | Non-viral |
| ZX002 | pEZX-FR02 | SV40 | Firefly luciferase (hLuc) | Renilla luciferase (Rluc) | N/A* | Ampicillin | Non-viral |
| ZX003 | pEZX-FR03 | miniCMV | Firefly luciferase (hLuc) | Renilla luciferase (Rluc) | Puromycin | Kanamycin | Non-viral |
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