– 高灵敏性萤火虫荧光素酶检测试剂盒
Luc-Pair™ Firefly Luciferase HS Assay Kit 是一款高灵敏性荧光素酶检测试剂盒，可高效、快速检测萤火虫（Firefly luciferase，FLuc）荧光素酶的活性。由于其具有极高的灵敏度，可检测极低水平表达、调控和转染不佳的细胞的表达，也可搭配微孔板使用。适用于 GeneCopoeia 及 Promega 的萤火虫荧光素酶表达载体。
产品特点
• 灵敏性高—本试剂盒对萤火虫荧光素酶产生的光信号极度敏感；
• 适用范围广—适用于多种不同的真核生物（ 脊椎动物、低等无脊椎动物）细胞系样品在微孔板（如：96孔板） 或单管中检测；
• 背景值低—背景更低，数据更准确；
• 操作简便—实验步骤简便；
• 重复性好—检测数据更可靠，且荧光素酶的浓度线性范围超过 9 个数量级。
同时，GeneCopoeia 还提供 Luc-Pair™ Duo-Luciferase Assay Kit 2.0（高稳定性双荧光素酶检测试剂盒）和 Luc-Pair™ Duo-Luciferase HS Assay Kit（高灵敏性双荧光素酶检测试剂盒）。
图1. 用 GeneCopoeia 的 Luc-Pair™ 高灵敏性萤火虫荧光素酶检测试剂盒检测萤火虫荧光素酶的活性。用 Promega 的 pGL4.13 报告载体转染 HEK293 细胞，转染 48 小时后检测荧光信号，结果如上图所示。Fluc-GeneCopoeia 代表 GeneCopoeia 的检测结果， Fluc-Promega 代表 Promega 品牌试剂盒的检测结果。
图2. 用 GeneCopoeia 的 Luc-Pair™ 高灵敏性萤火虫荧光素酶检测试剂盒检测萤火虫荧光素酶信号的稳定性。用 Promega 的 pGL4.13 报告载体转染 HEK293 细胞，转染 48 小时后检测荧光信号，FLuc（FLuc-GeneCopoeia）的活性如上图所示。用自动加样器分配 Fluc 工作液，设置震动混合为 1 秒，然后立即检测，检测时间为 20 秒。
| 购买 | 货号 | 产品 | 描述 | 价格(￥) |
| ZX003 | Fluc + Rluc reporter clone | pEZX-FR03 包含萤火虫荧光素酶(Fluc)和海肾荧光素酶(Rluc)的报告载体 | 3960 | |
| ZX001 | Fluc + Rluc reporter clone | pEZX-FR01 包含萤火虫荧光素酶(Fluc)和海肾荧光素酶(Rluc)的报告载体 | 3960 | |
| ZX002 | Fluc + Rluc reporter clone | pEZX-FR02 包含萤火虫荧光素酶(Fluc)和海肾荧光素酶(Rluc)的报告载体 | 3960 |
相关产品
Yang, Q., et al. (2023). N4‐Acetylcytidine Drives Glycolysis Addiction in Gastric Cancer via NAT10/SEPT9/HIF‐1α Positive Feedback Loop. Advanced Science doi: 10.1002/advs.202300898 [Lenti-Pac™ HIV Expression Packaging Kit; OMICSLINK™ lentiviral shRNA clone; miTarget™ 3′ UTR Clone; Luc-Pair™ Duo-Luciferase HS Assay Kit]
Yu, C., et al. (2023). Flot2 acts as a novel mediator of podocyte injury in proteinuric kidney disease. Int J Biol Sci doi: 10.7150/ijbs.78945 [OMICSLINK™ empty vector; GLuc-ON™ promoter reporter clone; Lenti-Pac™ HIV Expression Packing Kit; Secrete-Pair™ Dual Luminescence Assay Kit]
Sanmarco, L.M., et al. (2023). Lactate limits CNS autoimmunity by stabilizing HIF-1α in dendritic cells. Nature doi: 10.1038/s41586-023-06409-6 [OMICSLINK™ NDUFA4L2 ORF expression clone and empty control vector; Secrete-Pair™ Gaussia Luciferase Assay Kit]
Zhang, Y., et al. (2023). ZNF451 favors triple-negative breast cancer progression by enhancing SLUG-mediated CCL5 transcriptional expression. Cell reports doi: 10.1016/j.celrep.2023.112654 [GLuc-ON™ promoter-reporter clone; Secrete-Pair™ Luciferase Assay Kit]
Wei, J., et al. (2023). Targeting REGNASE-1 programs long-lived effector T cells for cancer therapy. Nature doi: 10.1038/s41586-019-1821-z [miTarget™ mouse Batf, Il2 and Il4 3′ UTR miRNA Target Clones; Luc-Pair™ Duo-Luciferase Assay Kits 2.0]
Ke, H., et al. (2023). Landscape of pathogenic mutations in premature ovarian insufficiency. Nature Medicine doi: 10.1038/s41591-022-02194-3 [OMICSLINK™ empty vector; Secrete-Pair™ dual luminescence kit]
Yang, Y.C., et al. (2023). The RNA-binding protein KSRP aggravates malignant progression of clear cell renal cell carcinoma through transcriptional inhibition and post-transcriptional destabilization of the NEDD4L ubiquitin ligase. J Biomed Sci doi: 10.1186/s12929-023-00949-9 [GLuc-ON™ promoter-reporter clone; Secrete-Pair™ Gaussia Luciferase Assay kit]
Wang, Z., et al. (2023). Extracellular vesicles in fatty liver promote a metastatic tumor microenvironment. Cell Metabolism doi: 10.1016/j.cmet.2023.04.013 [Luc-Pair™ Duo-Luciferase Assay Kits 2.0]
Lv, Y., et al. (2023). RBM46 is essential for gametogenesis and functions in post-transcriptional roles affecting meiotic cohesin subunits. doi: 10.1093/procel/pwac040 [Secrete-Pair™ Dual Luminescence Assay Kit]
Zhu, L., et al. (2023). A Zeb1/MtCK1 metabolic axis controls osteoclast activation and skeletal remodeling. The EMBO Journal doi: 10.15252/embj.2022111148 [Secrete Pair™ Gaussia Luciferase Assay Kit; Secrete-Pair™ Dual Luminescence Assay Kit]
Liu, H., et al. (2023). Development of novel SARS-CoV-2 viral vectors. Sci Rep doi: 10.1038/s41598-023-40370-8 [Luc-Lysis II Buffer]
Song. M., et al (2023). circIFNGR2 regulating ankylosing spondylitis-associated inflammation through macrophage polarization. iScience doi: 10.1016/j.isci.2023.107325 [miTarget™ 3′ UTR Clone; miTarget™ 3′ UTR miRNA Target Clones; Luc-Pair™ Duo-Luciferase HS Assay Kit]
Wasser, C., et al. (2023). Apoer2-ICD-dependent regulation of hippocampal ribosome mRNA loading. Res Sq doi: 10.21203/rs.3.rs-3040567/v1 [Gluc + SEAP reporter clone pEZX-GA01]
Yang, Y-C., et al. (2023). The RNA-binding protein KSRP aggravates malignant progression of clear cell renal cell carcinoma through transcriptional inhibition and post-transcriptional destabilization of the NEDD4L ubiquitin ligase. J Biomed Sci doi: 10.1186/s12929-023-00949-9 [OMICSLINK™ NEDD4L ORF expression clone; Secrete-Pair™ Gaussia Luciferase Assay kit]
Tsukui, D., et al. (2023). GM-CSF receptor/SYK/JNK/FOXO1/CD11c signaling promotes atherosclerosis. iScience doi: 10.1016/j.isci.2023.107293[OMICSLINK™ Lentiviral expression clone; Secrete-Pair™ Dual Luminescence Assay Kit]
Wischmann, F-J., et al. (2023). Tumor suppressor miR-218 directly targets epidermal growth factor receptor (EGFR) expression in triple-negative breast cancer; sensitizing cells to irradiation. J Cancer Res Clin Oncol doi: 10.1007/s00432-023-04750-x [Secrete-Pair™ Dual Luminescence Assay Kit; miTarget™ 3′ UTR Clone]
Wang, J., et al. (2023). Targeting exosomes enveloped EBV‐miR‐BART1‐5p‐antagomiRs for NPC therapy through both anti‐vasculogenic mimicry and anti‐angiogenesis. Cancer Med doi: 10.1002/cam4.5941 [Luc-Pair™ Duo-Luciferase HS Assay Kit]
Vyas, H., et al. (2023). miR34a-5p impedes CLOCK expression in chronodisruptive C57BL/6J mice and potentiates pro-atherogenic manifestations. PLoS One doi: 10.1371/journal.pone.0283591 [Secrete-Pair™ Dual Luminescence Assay Kit; miTarget™ 3′ UTR Clone]
Wang, Q., et al. (2023). A Novel Circ_Arf3/miR-452-5p/Mbnl1 Axis Regulates Proliferation and Expression of Fibrosis-Related Proteins of Mouse Mesangial Cells Under High Glucose. Diabetes Metab Syndr Obes doi: 10.2147/DMSO.S400530 [Secrete-Pair™ Dual Luminescence Assay Kit]
Jasiecki, J., et al. (2023). Novel Tools for Comprehensive Functional Analysis of LDLR (Low-Density Lipoprotein Receptor) Variants. Int J Mol Sci doi: 10.3390/ijms241411435 [Luc-Pair™ Duo-Luciferase Assay Kit 2.0]
Steadman, K., et al. (2023). Autonomous action and cooperativity between the ONECUT2 transcription factor and its 3′ untranslated region. Front Cell Dev Biol doi: 10.3389/fcell.2023.1206259 [Secrete-Pair™ Dual Luminescence Assay Kit]
Liu, H., et al. (2023). Isorhamnetin and anti-PD-L1 antibody dual-functional mesoporous silica nanoparticles improve tumor immune microenvironment and inhibit YY1-mediated tumor progression. J Nanobiotechnology doi: 10.1186/s12951-023-01967-3 [Secrete-Pair™ Dual Luminescence Assay Kit]
Pan, L., et al. (2023). Osteoclast-Derived Exosomal miR-5134-5p Interferes with Alveolar Bone Homeostasis by Targeting the JAK2/STAT3 Axis. Int J Nanomedicine doi: 10.2147/IJN.S413692 [Luc-Pair™ Duo-Luciferase HT Assay Kit]
Li, H., et al. (2023). FOXO3 regulates Smad3 and Smad7 through SPON1 circular RNA to inhibit idiopathic pulmonary fibrosis. Int J Biol Sci doi: 10.7150/ijbs.80140 [Secrete-Pair™ Dual Luminescence Assay Kit]
Liu, Y., et al. (2023). PTPN2 targets TAK1 for dephosphorylation to improve cellular senescence and promote adipose tissue browning in T2DM. Front Pharmacol doi: 10.3389/fphar.2023.1124633 [Luc-Pair™ Duo-Luciferase Assay Kit 2.0]
Yang, W., et al. (2023). MiR-363 restrain the proliferation; migration and invasion of colorectal carcinoma cell by targeting E2F3. J Cancer doi: 10.7150/jca.83897 [Luc-Pair™ Duo-Luciferase Assay Kit 2.0]
AN, N., et al. (2023). The zinc figure protein ZNF575 impairs colorectal cancer growth via promoting p53 transcription. Oncol Res doi: 10.32604/or.2023.028564 [Luc-Pair™ Duo-Luciferase HS Assay Kit]
Kalantzakos, T., et al. (2023). MicroRNA-155-5p Targets JADE-1; Promoting Proliferation; Migration; and Invasion in Clear Cell Renal Cell Carcinoma Cells. Int J Mol Sci doi: 10.3390/ijms24097825 [miArrest™ miRNA inhibitors; EndoFectin™ Lenti; Luc-Pair™ Duo-Luciferase Assay Kits 2.0]
Sun, B., et al. (2023). Engineered induced-pluripotent stem cell derived monocyte extracellular vesicles alter inflammation in HIV humanized mice. Extracell Vesicles Circ Nucl Acids doi: 10.20517/evcna.2022.11 [miTarget™ 3′ UTR Clone; Secrete-Pair™ Dual Luminescence Assay Kit ]
Zhang, J., et al. (2023). Naringenin in Si-Ni-San formula inhibits chronic psychological stress-induced breast cancer growth and metastasis by modulating estrogen metabolism through FXR/EST pathway. J Adv Res doi: 10.1016/j.jare.2022.06.006 [GLuc-ON™ promoter-reporter clone; Secrete-Pair™ Dual Luminescence Assay Kit]
Dykxhoorn, D.M., et al. (2023). MicroRNA-423-5p Mediates Cocaine-Induced Smooth Muscle Cell Contraction by Targeting Cacna2d2. Int J Mol Sci doi: 10.3390/ijms24076584 [pEZX-MT05 vector; miTarget™ Cacna2d2 3′ UTR Clone; Secrete-Pair™ Dual Luminescence Assay Kit]
Cui, X., et al. (2023). Hepatocellular carcinoma-derived FOXO1 inhibits tumor progression by suppressing IL-6 secretion from macrophages. Neoplasia doi: 10.1016/j.neo.2023.100900 [GLuc-ON™ promoter-reporter clone; Secrete-Pair™ Gaussia Luciferase Assay Kit]
Pang, N., et al. (2023). Nicotinamide Adenine Dinucleotide Precursor Suppresses Hepatocellular Cancer Progression in Mice. Nutrients doi: 10.3390/nu15061447 [EX-hLUC-Lv201; Luc-Pair™ Duo-Luciferase Assay Kit 2.0]
Zheng, Y., et al. (2023). Chronic psychological stress promotes breast cancer pre-metastatic niche formation by mobilizing splenic MDSCs via TAM/CXCL1 signaling. J Exp Clin Cancer Res doi: 10.1186/s13046-023-02696-z [GLuc-ON™ CXCL1 promoter-reporter clone; Luc-Pair™ Duo-Luciferase Assay Kit 2.0]
Xu, H-J., et al. (2023). ESE1/AGR2 axis antagonizes TGF‐β‐induced epithelial‐mesenchymal transition in low‐grade pancreatic cancer. Cancer Med doi: 10.1002/cam4.5397 [LucPair™ Duo‐Luciferase Assay Kit]
Salehi, R., et al. (2023). Androgen-induced exosomal miR-379-5p release determines granulosa cell fate: cellular mechanism involved in polycystic ovaries. J Ovarian Res doi: 10.1186/s13048-023-01141-1 [GLuc-ON™ PDK1 promoter-reporter clone; Luc-Pair™ Duo-Luciferase Assay Kits 2.0]
Shen, D., et al. (2023). Melatonin inhibits bladder tumorigenesis by suppressing PPARγ/ENO1-mediated glycolysis. Cell Death Dis doi: 10.1038/s41419-023-05770-8 [pGL3-ENO1-WT and pGL3-ENO1-Mut luciferase reporter plasmids; Luc-Pair™ Duo-Luciferase Assay Kits 2.0]
Li, Q., et al. (2023). MiR146a-loaded engineered exosomes released from silk fibroin patch promote diabetic wound healing by targeting IRAK1. Signal Transduct Target Ther doi: 10.1038/s41392-022-01263-w [Secrete-Pair™ Gaussia Luciferase Assay Kit]
Cheng, W., et al. (2023). lncRNA TPT1-AS1 promotes cell migration and invasion in esophageal squamous-cell carcinomas by regulating the miR-26a/HMGA1 axis. Open Med (Wars) doi: 10.1515/med-2022-0533 [Luc-Pair™ Duo-Luciferase Assay Kit 2.0]
Zhang, L., et al. (2023). Cytoplasmic Tail Truncation Stabilizes S1-S2 Association and Enhances S Protein Incorporation into SARS-CoV-2 Pseudovirions. J Virol doi: 10.1128/jvi.01650-22 [Luc-Pair™ Firefly Luciferase HS Assay Kit]
Liu, S., et al. (2023). C/EBPβ Coupled with E2F2 Promoted the Proliferation of hESC-Derived Hepatocytes through Direct Binding to the Promoter Regions of Cell-Cycle-Related Genes. Cells doi: 10.3390/cells12030497 [Luc-Pair™ Duo-Luciferase HS Assay Kit]
Li, F., et al. (2023). Reanalysis of ribosome profiling datasets reveals a function of rocaglamide A in perturbing the dynamics of translation elongation via eIF4A. Nat Commun doi: 10.1038/s41467-023-36290-w [miTarget™ miTarget™ 3′ UTR Clone]
Ke, H., et al. (2023). Landscape of pathogenic mutations in premature ovarian insufficiency. Nat Med doi: 10.1038/s41591-022-02194-3 [Secrete-Pair™ Dual Luminescence Assay Kit]
Mongiorgi, S., et al. (2023). A miRNA screening identifies miR-192-5p as associated with response to azacitidine and lenalidomide therapy in myelodysplastic syndromes. Clin Epigenetics doi: 10.1186/s13148-023-01441-9 [miTarget™ BCL2-3′ UTR miRNA Target Clones、Luc-Pair™ Duo-Luciferase Assay Kit 2.0]
LeDesma, R., et al. (2023). Structural features stabilized by divalent cation coordination within hepatitis E virus ORF1 are critical for viral replication. eLife doi: 10.7554/eLife.80529 [Luc-Pair™ Renilla Luciferase HS Assay Kit]
Takashi, M.Y., et al. (2023). Pyrolyzed deketene curcumin controls regulatory T cell generation and gastric cancer metabolism cooperate with 2-deoxy-d-glucose. Front Immunol doi: 10.3389/fimmu.2023.1049713 [Luc-Pair™ Duo-Luciferase Assay Kit 2.0]
Kuang, Y., et al. (2023). The developmental regulator HAND1 inhibits gastric carcinogenesis through enhancing ER stress apoptosis via targeting CHOP and BAK which is augmented by cisplatin. Int J Biol Sci doi: 10.7150/ijbs.76345 [Secrete-Pair™ Dual Luminescence Assay Kit]
Han, X., et al. (2023). miR-1275 targets MDK/AKT signaling to inhibit breast cancer chemoresistance by lessening the properties of cancer stem cells. Int J Biol Sci doi: 10.7150/ijbs.74227 [Luc-Pair™ Duo-Luciferase HS Assay Kit]
Riera, E., et al. (2023). African swine fever virus ubiquitin-conjugating enzyme pI215L inhibits IFN-I signaling pathway through STAT2 degradation. Front Microbiol doi: 10.3389/fmicb.2022.1081035 [Luc-Pair™ Duo-Luciferase HS Assay Kit]
Palakurti, R., et al. (2023). Inducible miR-1224 silences cerebrovascular Serpine1 and restores blood flow to the stroke-affected site of the brain. Mol Ther Nucleic Acids doi: 10.1016/j.omtn.2022.12.019 [Secrete-Pair™ Dual Luminescence Assay Kit]
Becker, V., et al. (2023). Hypoxia-induced downregulation of microRNA-186-5p in endothelial cells promotes non-small cell lung cancer angiogenesis by upregulating protein kinase C alpha. Mol Ther Nucleic Acids doi: 10.1016/j.omtn.2023.01.015 [Luc-Pair™ Duo-Luciferase HS Assay Kit]
Zheng, Z., et al. (2023). Mir155 regulates osteogenesis and bone mass phenotype via targeting S1pr1 gene. eLife doi: 10.7554/eLife.77742 [Luc-Pair™ Duo-Luciferase Assay Kit 2.0]
Li, Z., et al. (2023). Circ_0020256 induces fibroblast activation to drive cholangiocarcinoma development via recruitment of EIF4A3 protein to stabilize KLF4 mRNA. Cell Death Discov doi: 10.1038/s41420-023-01439-5 [All-in-One™ First-Strand cDNA Synthesis Kit; All-in-One™ qPCR Mix]
Mao,Y., et al. (2023). Identification Invasion-Related Long Non-Coding RNAs in Lung Adenocarcinoma and Analysis of Competitive Endogenous RNA Regulatory Networks. Int J Gen Med doi: 10.2147/IJGM.S407266 [SureScript™ First-Strand cDNA Synthesis Kit; BlazeTaq™ SYBR® Green qPCR mix 2.0]
