复能易锦提供的全部人类和小鼠ORF克隆及相关慢病毒和AAV病毒，所提供的载体质粒均采用毛细管电泳sanger全长测序验证，并承诺编码的氨基酸序列与NCBI数据库匹配。 All human and mice ORF clones and related Lentivirus, AAV particles and recombinant proteins provided by iGeneBio are fully sequenced using Sanger sequencing by capillary electrophoresis and Amino Acid Sequences are guaranteed to be matched with NCBI database.
Anti-GST (glutathione-S-transferase) tag antibody is a purified mouse IgG2a monoclonal antibody that binds to GST-fusion proteins. Anti-GST tag antibody can be used to identify and capture fusion proteins by western blots and immunoprecipita-tion. Immobilized anti-GST is also used for affinity purification of GST fusion proteins. OmicsLink™ anti-tag antibodies are ideal for use with OmicsLink™ expression-ready ORF cDNA clones.
The OmicsLink™ anti-GST tag antibody was produced by immunizing mice with a synthetic peptide of Schistosoma japonicum GST.
Specificity
The anti-GST tag monoclonal antibody detects over-expressed GST fusion proteins.
Figure 1. 20 ng of recombinant GST protein (lane 1) and 20 µg of 293 cell lysates were transfected with a GST expression vector and probed with OmicsLink Anti-GST (1 µg/ml).
Formulation
0.25 mg/ml in PBS, pH 7.4 with 0.05% sodium azide and 50% (v/v) glycerol.
Applications
Western blot, immunoprecipitation, immunostaining and affinity purification.
Storage/Stability
Stable for 1 year at -20°C from shipment date. Briefly centrifuge the original vial prior to opening the cap to collect the product at the bottom of the tube.
Starting dilution recommendations
Western blot at 1:1000
immunoprecipitation and immunostaining at 1:200
