复能易锦提供的全部人类和小鼠ORF克隆及相关慢病毒和AAV病毒，所提供的载体质粒均采用毛细管电泳sanger全长测序验证，并承诺编码的氨基酸序列与NCBI数据库匹配。 All human and mice ORF clones and related Lentivirus, AAV particles and recombinant proteins provided by iGeneBio are fully sequenced using Sanger sequencing by capillary electrophoresis and Amino Acid Sequences are guaranteed to be matched with NCBI database.
Anti-mCherry antibody is a purified mouse IgG monoclonal antibody that binds to mCherry protein. Anti-mCherry antibody can be used to identify and capture mCherry or fusion proteins by western blots and immunoprecipitation. Immobilized anti-mCherry can also be used for affinity purification of mCherry fusion proteins. OmicsLink™ anti-tag antibodies are ideal for use with OmicsLink™ expression-ready ORF cDNA clones.
The OmicsLink™ anti-mCherry antibody was raised against a purified Red Fluorescent Protein (RFP, also known as DsRed) of Discosoma sp. origin.
Specificity
The anti-mCherry monoclonal antibody recognizes RFP (DsRed) and its variants including mCherry, plum, and probably other RFP-derived fluorescent proteins.
Figure 1. mCherry western blot analysis of 293 cells transfected with a fusion construct which contains a mCherry tag.
Formulation
0.25 mg/ml in PBS, pH 7.2 with 0.1% sodium azide and 50% (v/v) glycerol.
Applications
Western blot, ELISA, immunoprecipitation, immunostaining and affinity purification.
Storage/Stability
Stable for 1 year at -20°C from shipment date. Briefly centrifuge the original vial prior to opening the cap to collect the product at the bottom of the tube.
Starting dilution recommendations
Optimal working conditions should be determined by the user. General recommendations are western blot at 1:1,000; immunoprecipitation at 5 µg/mg lysate and immunostaining at 1:200.
