复能易锦提供的全部人类和小鼠ORF克隆及相关慢病毒和AAV病毒，所提供的载体质粒均采用毛细管电泳sanger全长测序验证，并承诺编码的氨基酸序列与NCBI数据库匹配。 All human and mice ORF clones and related Lentivirus, AAV particles and recombinant proteins provided by iGeneBio are fully sequenced using Sanger sequencing by capillary electrophoresis and Amino Acid Sequences are guaranteed to be matched with NCBI database.
Anti-D tag antibody is a purified mouse IgG2b monoclonal antibody that binds to D epitope (DYKDDDDK, also known as FLAG®)-tagged fusion proteins. Anti-D tag antibody can be used to identify and capture fusion proteins by western blots and immunoprecipitation. Immobilized anti-D is also used for affinity purification of D-tag fusion proteins. OmicsLink™ anti-tag antibodies are ideal for use with OmicsLink™ expression-ready ORF cDNA clones.
The OmicsLink™ anti-D tag antibody was produced by immunizing mice with a synthetic peptide (DYKDDDDK) coupled to KLH.
Specificity
The anti-D tag antibody recognizes over-expressed recombinant proteins containing the D epitope tag (DYKDDDDK) fused to either the N- or C-terminal of targeted proteins in transfected mammalian cells or other expression systems.
Figure 1.Western blot analysis using 293 cells transfected with D tag vectors. Lane1: highest concentration at 0.5 mg/ml Anti-D tag antibody, Lane 4: lowest concentration at 0.1 µg/ml.
Formulation
0.25 mg/ml in PBS, pH 7.2 with 0.05% sodium azide and 50% (v/v) glycerol.
Applications
Western blot, immunoprecipitation, immunostaining and affinity purification.
Storage/Stability
Stable for 1 year at -20°C from shipment date. Briefly centrifuge the original vial after prior to opening the cap to collect the product at the bottom of the tube.
Starting dilution recommendations
Optimal working conditions should be determined by the user. General recommendations are western blot at 1:1000;
immunoprecipitation and immunostaining at 1:200.
