All-in-One™ qPCR Mix
All-in-One™ qPCR Mix
All-in-One™ qPCR Mix
产品介绍
All-in-One 编码基因验证引物
All-in-One qPCR mix试剂与基因特异引物在通用的qPCR反应条件下配套使用，即可获得可靠的实验数据。两种产品经共同开发并进行一体化优化，表现出了如您所期望的、无需任何高成本的全范围优势。
- 均一化的反应条件缩短实验设计时间，更早提交论文
- 高扩增效率和灵敏度（甚至是低丰度基因）意味着每次实验中的可靠定量
- 无非特异性扩增和二聚体，确保可重复性和数据可靠性
All-in-One qPCR mix使用高保真热启动酶、经过优化的反应缓冲液和高质量dNTPs，能够使低丰度RNA（cDNA）或DNA进行特异的、灵敏的扩增。
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| 扩增曲线 | 熔解曲线 | 标准曲线 |
图1.用梯度稀释的质粒DNA（5×106至5个分子）做标准曲线来评估All-in-One™ qPCR Mix的扩增效率和检测灵敏度。如扩增曲线和熔解曲线所示，All-in-One™ qPCR Mix可扩增低至5个分子的模板（质粒DNA）说明了扩增和检测的高灵敏度。
All-in-One 编码基因验证引物 
使用GeneCopoeia精确的qPCR试剂产品可消除繁冗的调整和优化过程。只需选择All-in-One验证引物即可开始。
All-in-One人、小鼠或大鼠的qPCR反应特异引物由专利算法设计，并为准确定量而进行了验证。验证结果包括熔解曲线，以保证扩增正确靶DNA序列（见图2.）。
与基于SYBR® Green I的All-in-One qPCR Mix试剂配套使用，All-in-One验证引物在qPCR定量实验中表现出可靠、可重复的高效性。
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| 扩增曲线 | |
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| 熔解曲线 | 验证结果的琼脂糖电泳 |
图2. 45对基因特异引物经过实验验证均产生单一扩增产物，融解曲线均呈单一锐锋，电泳条带均呈单一条带，且与目标基因相符。由10种人组织（肺、肝、睾丸、卵巢、脾、脑、胎盘、胰、心和乳腺）混合样本的总RNA经逆转录形成的cDNA作为引物验证的模板。qPCR反应由终浓度0.2 uM的引物和All-in-One qPCR Mix配合完成。使用Bio-Rad iQ5 qPCR仪器，在95°C预变性10分钟后，紧接着40个PCR循环（95°C变性10秒，60°C退火20秒钟，72°C延伸15秒），最后一个循环后设置72至95°C的熔解曲线检测程序。扩增曲线、熔解曲线和琼脂糖凝胶电泳图如图所示（验证的阳性结果见奇数泳道，阴性对照结果（NTC）见偶数泳道）。
注释：1.以上无非特异性扩增和二聚体的验证结果均建立于本公司配套试剂产品和反应体系之上。2.以上验证引物包括人、小鼠和大鼠，如需其他物种请咨询。
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发表的文章
- Quipildor, G.E.F., et al. (2026). Differential downstream signaling in microglia lacking Alzheimer’s-related TREM2 or its adaptor TYROBP/DAP12. Molecular Neurodegeneration Advances DOI: 10.1186/s44477-025-00012-x [All-in-One™ qPCR Mix, Cat. No. QP001]
- Madhu, L.N., et al. (2026). Intranasal Human NSC‐Derived EVs Therapy Can Restrain Inflammatory Microglial Transcriptome, and NLRP3 and cGAS‐STING Signalling, in Aged Hippocampus. Journal of Extracellular Vesicles DOI: 10.1002/jev2.70232 [All-in-One™ mRNA qPCR Primers]
- Zhang, H., et al. (2026). METTL7B-stabilized lncRNA-MIR22HG to drive p53-mediated neuronal apoptosis via the ubiquitinating JARID2 in cerebral ischemia/reperfusion injury. Cell Biology and Toxicology DOI: 10.1007/s10565-026-10157-4 [All-in-One™ qPCR Mix, Cat. No. QP001]
- Wang, L., et al. (2026). Plasma Exosomal LncRNA XIST Impairs CD4+ T Cell Autophagy and Promotes Activation via the miR-98/RICTOR/Akt-mTOR Axis in Systemic Lupus Erythematosus. Journal of Inflammation Research DOI: 10.2147/JIR.S574715 [All-in-One™ miRNA First-Strand cDNA Synthesis Kit 2.0; All-in-One™ miRNA qRT-PCR Detection Kit 2.0; All-in-One™ miRNA qPCR Primer against mature miRNA hsa-miR-98, U48]
- Cheng, C., et al. (2026). MiR-1290 in natural killer cell derived extracellular vesicles: a pathogenic mediator of lupus nephritis and therapeutic target for th17 regulation. Journal of Nanobiotechnology DOI: 10.1186/s12951-026-04212-9 [All-in-One™ miRNA qPCR Primer]
- Deng, Y., et al. (2026). Vaccinia Virus Capping Enzyme Subunit D12 Is Differentially Required for Viral Replication in Different Cell Lines and Gastrointestinal Organoids. Journal of medical virology DOI: 10.1002/jmv.70852 [All-in-One™ qPCR Mix]
- Chi, L., et al. (2026). CSF1R T567M mutation induces microglial dysfunction and synaptic impairment in patient iPSC-derived cerebral organoids of CSF1R-related disorder. Cell Death Discovery DOI: 10.1038/s41420-026-02995-2 [All-in-One™ qPCR Mix]
- Hu, J., et al. (2026). miR-34a targets MTUS1 to regulate cardiac fibrosis in rats. Journal of Central South University Medical Sciences DOI: 10.11817/j.issn.1672-7347.2026.250600 [All-in-One™ miRNA qPCR Primer against mature miRNA rno-mir-34a-5p, U6, GAPDH, MTUSl and collagen I, Cat. No. RmiRQP0440, RmiRQP9003, RQP049537, RQP053351, RQP054226]
- ZHAO, J., et al. (2026). Pelargonidin Inhibits Isoproterenol Induced Myocardial Fibrosis via Regulating Transforming Growth Factor-β/Smad2/3 Signaling and Th2 Cytokines in Mice. Physiological Research DOI: 10.33549/physiolres.935673 [All-in-One™ qPCR Mix, Cat. No. QP001]
- Tao, D., et al. (2026). Low PTGDS Expression Facilitates HNSCC by Suppressing Programmed Cell Death and Reducing B Cell–Mediated Immune Responses. Mediators of Inflammation DOI: 10.1155/mi/4521847 [All-in-One™ miRNA Universal Adaptor PCR Primer]
- Jiang, B., et al. (2026). SLC7A7 Downregulation in Monocytes Drives Immunosuppression and Osteosarcoma Progression. International Journal of Genomics DOI: 10.1155/ijog/8314828 [All-in-One™ miRNA qRT-PCR Detection Kit 2.0, Cat. No. QP115; All-in-One™ miRNA Universal Adaptor PCR Primer]
- Samah, N., et al. (2026). Expression Profiles of α1nAChR, ERK1/2, c-FOS and Matrix Metalloproteinases Among Male Smokers with Acute Coronary Syndrome. International Journal of Molecular Sciences DOI: 10.3390/ijms27114757 [All-in-One™ qPCR mix with validated primers, Cat. No. Hs-QRP-40509, Hs-QRP-41066, Hs-QRP-21506, Hs-QRP-20169]
- Mansour, M., et al. (2026). Molecular Signature of Cisplatin Resistance in Ovarian Cancer Identifies Therapeutic Opportunities for Re-sensitization. Journal of Cancer DOI: 10.7150/jca.124252 [All-in-One™ qPCR Primers for Human ZBTB33, NR1I2, NFKB1, ERBB2, ABCB1, HIF1A, ACTB]
- Quipildor, G.E.F., et al. (2026). Differential downstream signaling in microglia lacking Alzheimer’s-related TREM2 or its adaptor TYROBP/DAP12. Molecular Neurodegeneration Advances DOI:10.1186/s44477-025-00012-x [All-in-One™ qPCR Mix, Cat. No. QP004]
- Madhu, L.N., et al. (2026). Intranasal Human NSC‐Derived EVs Therapy Can Restrain Inflammatory Microglial Transcriptome, and NLRP3 and cGAS‐STING Signalling, in Aged Hippocampus. Journal of Extracellular Vesicles DOI:10.1002/jev2.70232 [All-in-One™ qPCR Primer for Ndufs6 and Ndufs7 (Complex I), Sdha and Sdhb (Complex II), Bcs1l and Cyc1 (Complex III); Cox4i2 and Cox7b (Complex IV), Atp6ap1 (Complex V) and Slc25a10; All-in-One™ qPCR Primer for Reference Gene GAPDH, Cat. No. HQP006940]
- Zhang, H., et al. (2026). METTL7B-stabilized lncRNA-MIR22HG to drive p53-mediated neuronal apoptosis via the ubiquitinating JARID2 in cerebral ischemia/reperfusion injury. Cell Biology and Toxicology DOI: 10.1007/s10565-026-10157-4 [All-in-One™ qPCR Mix, Cat. No. QP001]
- Wang, L., et al. (2026). Plasma Exosomal LncRNA XIST Impairs CD4+ T Cell Autophagy and Promotes Activation via the miR-98/RICTOR/Akt-mTOR Axis in Systemic Lupus Erythematosus. Journal of Inflammation Research DOI: 10.2147/JIR.S574715 [All-in-One™ qPCR Primer for Mouse Tut1, Cat. No. MQP042953; All-in-One™ miRNA qRT-PCR Detection Kit 2.0, Cat. No. QP115]
- Cheng, C., et al. (2026). MiR-1290 in natural killer cell derived extracellular vesicles: a pathogenic mediator of lupus nephritis and therapeutic target for th17 regulation. Journal of Nanobiotechnology DOI: 10.1186/s12951-026-04212-9 [All-in-One™ miRNA qPCR Primer against mature miRNA hsa-miR-1303, hsa-miR-329-3p, hsa-miR-338-5p, hsa-miR-630, hsa-miR-518e-3p, hsa-miR-659-3p, hsa-miR-300, hsa-miR-203a-3p, hsa-miR-92a-3p and hsa-miR-194-5p, Cat. No. HmiRQP0150, HmiRQP0416, HmiRQP0425, HmiRQP0740, HmiRQP0585, HmiRQP0770, HmiRQP1165, HmiRQP0377, HmiRQP0305, HmiRQP0832, HmiRQP0280]
- Volbracht, C., et al. (2025). Deubiquitinating Enzymes Ubiquitin-Specific Proteases 7 and 10 Regulate TAU Aggregation. International Journal of Molecular Sciences DOI: 10.3390/ijms262211062 [All-in-One™ qPCR primers for Usp7 and Senp3]
- Yang, X., et al. (2025). LncRNA NONHSAT248596.1 Promotes Osteoarthritis via miR-146a-5p/CXCR4 Axis by Inducing Chondrocyte Apoptosis and Extracellular Matrix Degradation. Journal of Inflammation Research DOI: 10.2147/JIR.S558704 [All-in-One™ qPCR Mix]
- Zhu, X., et al. (2025). Sleep fragmentation exacerbates myocardial ischemia-reperfusion injury via hypothalamic paraventricular nucleus-resident OX1R-mediated sympathetic hyperactivity in adult mice. Annals of Medicine DOI: 10.1080/07853890.2025.2599059 [All-in-One™ qPCR Mix, Cat. No. QP001]
- Pant, A., et al. (2025). Ciclopirox suppresses poxvirus replication by targeting iron metabolism. Antiviral research DOI: 10.1016/j.antiviral.2025.106290 [All-in-One™ qPCR Mix]
- Pant, A., et al. (2025). Discovery and Characterization of a Hydroxypyridone-3-carboxamide Analog as an Antiviral Lead against Orthopoxviruses. bioRxiv DOI: 10.64898/2025.12.18.695249 [All-in-One™ qPCR Mix]
- Wang, S., et al. (2025). Structural Basis of TACO1-Mediated Efficient Mitochondrial Translation. bioRxiv DOI: 10.64898/2025.12.18.695269 [All-in-One™ qPCR Primers for Human ACTB]
- Kipcak, S., et al. (2025). Epithelial Barrier Function and Altered Cell Signaling Pathways in the Esophageal Epithelium of Achalasia Patients. The Turkish Journal of Gastroenterology DOI: 10.5152/tjg.2025.25031 [All-in-One™ qPCR Primer for CDH1, CLDN1, CLDN4, TJP1, Tjp2, OCLN]
- Bariani, M.V., et al. (2025). Mechanical Stiffness Influences the Response of Human Uterine Fibroid Cells to Hormonal Treatments. Reproductive Sciences DOI: 10.1007/s43032-025-02016-0 [All-in-One™ qPCR Primer for Human ITGA5, ROCK1 and MYLK, Cat. No. HQP009799, HQP016371, HQP157344]
- Wang, J., et al. (2025). MiR-142-3p targets the CXCL12/WNT/β-catenin pathway to regulate the stemness of breast cancer cells. Scientific Reports DOI: 10.1038/s41598-025-34163-4 [All-in-One™ qPCR Mix, Cat. No. QP004; All-in-One™ miRNA qRT-PCR Detection Kit 2.0, Cat. No. QP115]
- Yang, Y., et al. (2025). Periostin mediates collagen production; ECM remodeling and myofibroblast differentiation in breast prosthesis capsule formation. Sci Rep DOI: 10.1038/s41598-025-11409-9 [Periostin expression and interference vectors; Lenti-Pac™ HIV Expression Packaging Kit, Cat. No. LT001; All-in-One™ First-Strand-cDNA Synthesis Kit; All-in-One™ qPCR Mix]
- Lin, L., et al. (2025). The ESRP1 promoter reporter can function as an in vivo sensor of DNA methyltransferase inhibition. BMC Biotechnol DOI: 10.1186/s12896-025-01031-y [All-in-One™ miRNA First-Strand cDNA Synthesis Kit 2.0, Cat. No. QP113; All-in-One™ qPCR Primer for Human ESRP1 and Reference Gene GAPDH]
- Chen, Y., et al. (2025). Dysregulation of adipocytokines via the hsa-miR-548ay-3p/PPARγ signaling pathway leads to cardiac fibrosis. Sci Rep DOI: 10.1038/s41598-025-11324-z [miProfile™ Human MSC exosome miRNA qPCR Arrays; All-in-One™ qPCR Mix, Cat. No. QP001; All-in-One™ miRNA qRT-PCR Detection Kit 2.0, Cat. No. QP115]
- Xiong, S., et al. (2025). PLGA-microspheres carried circ_0003251 alleviate intervertebral disc degeneration via the miR-637/AKT1 axis. J Transl Med DOI: 10.1186/s12967-025-06800-z [All-in-One™ miRNA First-Strand cDNA Synthesis Kit, Cat. No. QP113; All-in-One™ qPCR Mix, Cat. No. QP001]
- Cao, Q., et al. (2025). Role of cellular prion protein in mouse granulosa cells and its effects on ovarian function in knockout mice. Mol Med Rep DOI: 10.3892/mmr.2025.13630 [All-in-One™ miRNA qPCR Primers Mouse Prnp, Cat. No. MQP094390]
- Yin, Q., et al. (2025). Serum miR-146a as a diagnostic and predictive biomarker for osteoporosis and bisphosphonate treatment efficacy. Am J Transl Res DOI: 10.62347/JRIQ2431 [All-in-One™ miRNA qPCR Primer]
- Wang, S-H., et al. (2025). Unveiling the mechanisms driving the rapid growth of Malania oleifera seedlings; a high-value root hemiparasitic plant. Front Plant Sci DOI: 10.3389/fpls.2025.1589651 [All-in-One™ qPCR Mix]
- Pant, A., et al. (2025). Ciclopirox suppresses poxvirus replication by targeting iron metabolism. bioRxiv DOI: 10.1101/2025.07.24.666650 [All-in-One™ qPCR Mix]
- Xu, B., et al. (2025). Regulation of RHBDD1 in the invasion of esophageal cancer cells via ELK3/Wnt/β-catenin signaling pathway. Front Bioeng Biotechnol DOI: 10.3389/fbioe.2025.1604859 [All-in-One™ qPCR primer]
- Chia, S-Y., et al. (2025). Single-nucleus transcriptomics reveals a distinct microglial state and increased MSR1-mediated phagocytosis as common features across dementia subtypes. Genome Med DOI: 10.1186/s13073-025-01519-4 [All-in-One™ qPCR Mix]
- Dong, Z., et al. (2025). Inhibition and Rescue of Hyperglycemia‐Induced Cellular Senescence by Mitochondrial Transfer from Enucleated Mesenchymal Stem Cell‐Derived Microvesicles for Chronic Wound Healing. Adv Sci (Weinh) DOI: 10.1002/advs.202501612 [All-in-One™ qPCR Mix]
- Xu, H., et al. (2025). Platelet-Rich Fibrin Promotes Osteoblast Recruitment-Associated Periodontal Regeneration via Macrophage Polarization. Stem Cells Int DOI: 10.1155/sci/9912405 [SureScript™ First-Strand cDNA Synthesis Kit, Cat. No. QP056; All-in-One™ qPCR Mix, Cat. No. QP001]
- Huang, W., et al. (2025). Construction and validation of a necroptosis-related lncRNA signature for predicting the prognosis of gastrointestinal cancer patients. Front Immunol DOI: 10.3389/fimmu.2025.1591252 [All-in-One™ miRNA qPCR Primers; SureScript™ First-Strand cDNA Synthesis Kit, Cat. No. QP056; BlazeTaq™ SYBR Green qPCR Mix 2.0, Cat. No. QP031]
- Zhao, M-Z., et al. (2025). IFN-γ downregulates miR-4319 to enhance NLRC5 and MHC-I expression in MHC-I-deficient breast cancer cells. Cancer Biol Ther DOI: 10.1080/15384047.2025.2523621 [All-in-One™ miRNA qPCR Primers HLA-A, HLA-B, HLA-C and GAPDH, Cat. No. HQP008849, HQP008850, HQP008851, HQP064347]
- Liu, L., et al. (2025). Hsa-miR-21 promoted the progression of lung adenocarcinoma by regulating LRIG1 expression. BMC Pulm Med DOI: 10.1186/s12890-025-03620-1 [All-in-One™ qPCR Mix, Cat. No. QP001]
- Ma, T., et al. (2025). MSTN Regulates Bovine Skeletal Muscle Satellite Cell Differentiation via PSMA6-Mediated AKT Signaling Pathway. Int J Mol Sci DOI: 10.3390/ijms26114963 [All-in-One™ qPCR Mix, Cat. No. QP001]
- Xiu, X., et al. (2025). Synergistic potential of bone marrow mesenchymal stem cells and miR181-a combinational therapy against multiple sclerosis. Stem Cell Res Ther DOI: 10.1186/s13287-025-04401-7 [293T human epithelial cell line; All-in-One™ miR First-Strand cDNA Synthesis Kit, Cat. No. QP113; All-in-One™ miRNA Universal Adaptor PCR Primer, Cat. No. QP029]
- Cao, Y., et al. (2025). miR-141-3p-loaded extracellular vesicles ameliorate intrahepatic bile duct stone disease by decreasing MUC5AC expression via the MAPK pathway. Intractable Rare Dis Res DOI: 10.5582/irdr.2024.01051 [miArrest™ miRNA inhibitor clone targeting hsa-miR-141-3p; All-in-One™ miRNA qPCR Primers hsa-miR-141-3p, Cat. No. HmiRQP0184, HmiRQP9001]
- Deng, C., et al. (2025). Sequential Targeting Chondroitin Sulfate‐Bilirubin Nanomedicine Attenuates Osteoarthritis via Reprogramming Lipid Metabolism in M1 Macrophages. Adv Sci (Weinh) DOI: 10.1002/advs.202411911 [ All-in-One™ qPCR Mix]
- Rao, S., et al. (2025). Extracellular vesicles from hiPSC-derived NSCs protect human neurons against Aβ-42 oligomers induced neurodegeneration; mitochondrial dysfunction and tau phosphorylation. Stem Cell Res Ther DOI: 10.1186/s13287-025-04324-3 [All-in-One™ qPCR Primers]
- Dsouza, L., et al. (2025). Vaccinia growth factor-dependent modulation of the mTORC1-CAD axis upon nutrient restriction. J Virol DOI: 10.1128/jvi.02110-24 [All-in-One™ qPCR Mix]
- Li, X., et al. (2025). The diagnostic value of a breast cancer diagnosis model based on serum MiRNAs and serum tumor markers. World J Surg Oncol DOI: 10.1186/s12957-025-03719-z [All-in-One™ miRNA qPCR Primers]
- Qi, T., et al. (2025). SlMYB72 and SlMYB75 antagonistically regulate trichome formation via the MYB-bHLH-WD40 complex in tomato. J Biol Chem DOI: 10.1016/j.jbc.2025.108313 [All-in-One™ qPCR Mix]
- Yusuf, A.N.M., et al. (2025). Dysregulation of Leukaemia Inhibitory Factor (LIF) Signalling Pathway by Supraphysiological Dose of Testosterone in Female Sprague Dawley Rats During Development of Endometrial Receptivity. Biomedicines DOI: 10.3390/biomedicines13020289 [All-in-One™ miRNA qPCR Primers LIFR, LIF, JAK1, STAT3, MUC1 and GAPDH, Cat. No. RQP050963, RQP050332, RQP067291, RQP049074, CS-RQP2143L-01, RQP050332]
- Fan, W., et al. (2025). Insights From m6A RNA Methylation: Biomarkers for Diagnosis of Acute Myocardial Infarction. J Inflamm Res DOI: 10.2147/JIR.S512476 [All-in-One™ miRNA qPCR Primers METTL3, WTAP, FTO, ALKBH5, FMR1, IGF2BP1 and IGF2BP1, Cat. No. HQP015070, HQP022953, HQP111736, HQP013831, HQP005998, HQP000777, HQP006940]
- Chen, W., et al. (2025). Analysis of the effect of ALA-PDT on macrophages in footpad model of mice infected with Fonsecaea monophora based on single-cell sequencing. Open Med (Wars) DOI: 10.1515/med-2024-1132 [All-in-One™ qPCR Mix]
- Xia, H-F., et al. (2025). PCBP2-dependent secretion of miRNAs via extracellular vesicles contributes to the EGFR-driven angiogenesis. Theranostics DOI: 10.7150/thno.102391 [All-in-One™ qPCR Primer]
- Ding, J., et al. (2025). LncRNAs in serum-derived extracellular vesicles are potential biomarker and correlated with immune infiltration in gastric cancer. Front Immunol DOI: 10.3389/fimmu.2025.1533111 [All-in-One™ First-Strand cDNA Synthesis Kit, Cat. No. QP006; All-in-One™ qPCR Mix, Cat. No. QP001]
