操作流程

Figure 1.不同缓冲体系中GLuc的信号稳定性比较。细胞转染人类Gluc报告基因克隆后收集细胞培养物，每个反应10μl。根据试剂盒的实验步骤进行操作，对每个试剂盒中两种缓冲体系进行测试，获得的信号百分比（Y轴）作为信号稳定性的指标。实验结果如图所示，在两种试剂盒中（Secrete-Pair和竞争者Gaussia luciferase assay kit），含有稳定剂（-S）的缓冲体系中Gluc的活性比不含稳定剂(-H)的缓冲体系中稳定。不仅如此，Secrete-Pair试剂盒缓冲体系中Gluc信号比竞争者试剂盒中稳定性更高（实验开始10分钟内近90%的信号可被捕获）。

Figure 2. GLuc和SEAP分析。细胞转染GLuc-SEAP双报告基因克隆后收集细胞培养物，每个反应10μl。反应初期，GL-H缓冲液中wtGLuc活性比GL-S缓冲液中高3-5倍。但GL-S缓冲液中GLuc活性更稳定。

Yang, Q., et al. (2023). N4‐Acetylcytidine Drives Glycolysis Addiction in Gastric Cancer via NAT10/SEPT9/HIF‐1α Positive Feedback Loop. Advanced Science doi: 10.1002/advs.202300898 [Lenti-Pac™ HIV Expression Packaging Kit; OMICSLINK™ lentiviral shRNA clone; miTarget™ 3′ UTR Clone; Luc-Pair™ Duo-Luciferase HS Assay Kit]

Yu, C., et al. (2023). Flot2 acts as a novel mediator of podocyte injury in proteinuric kidney disease. Int J Biol Sci doi: 10.7150/ijbs.78945 [OMICSLINK™ empty vector; GLuc-ON™ promoter reporter clone; Lenti-Pac™ HIV Expression Packing Kit; Secrete-Pair™ Dual Luminescence Assay Kit]

Sanmarco, L.M., et al. (2023). Lactate limits CNS autoimmunity by stabilizing HIF-1α in dendritic cells. Nature doi: 10.1038/s41586-023-06409-6 [OMICSLINK™ NDUFA4L2 ORF expression clone and empty control vector; Secrete-Pair™ Gaussia Luciferase Assay Kit]

Zhang, Y., et al. (2023). ZNF451 favors triple-negative breast cancer progression by enhancing SLUG-mediated CCL5 transcriptional expression. Cell reports doi: 10.1016/j.celrep.2023.112654 [GLuc-ON™ promoter-reporter clone; Secrete-Pair™ Luciferase Assay Kit]

Wei, J., et al. (2023). Targeting REGNASE-1 programs long-lived effector T cells for cancer therapy. Nature doi: 10.1038/s41586-019-1821-z [miTarget™ mouse Batf, Il2 and Il4 3′ UTR miRNA Target Clones; Luc-Pair™ Duo-Luciferase Assay Kits 2.0]

Ke, H., et al. (2023). Landscape of pathogenic mutations in premature ovarian insufficiency. Nature Medicine doi: 10.1038/s41591-022-02194-3 [OMICSLINK™ empty vector; Secrete-Pair™ dual luminescence kit]

Yang, Y.C., et al. (2023). The RNA-binding protein KSRP aggravates malignant progression of clear cell renal cell carcinoma through transcriptional inhibition and post-transcriptional destabilization of the NEDD4L ubiquitin ligase. J Biomed Sci doi: 10.1186/s12929-023-00949-9 [GLuc-ON™ promoter-reporter clone; Secrete-Pair™ Gaussia Luciferase Assay kit]

Wang, Z., et al. (2023). Extracellular vesicles in fatty liver promote a metastatic tumor microenvironment. Cell Metabolism doi: 10.1016/j.cmet.2023.04.013 [Luc-Pair™ Duo-Luciferase Assay Kits 2.0]

Lv, Y., et al. (2023). RBM46 is essential for gametogenesis and functions in post-transcriptional roles affecting meiotic cohesin subunits. doi: 10.1093/procel/pwac040 [Secrete-Pair™ Dual Luminescence Assay Kit]

Zhu, L., et al. (2023). A Zeb1/MtCK1 metabolic axis controls osteoclast activation and skeletal remodeling. The EMBO Journal doi: 10.15252/embj.2022111148 [Secrete Pair™ Gaussia Luciferase Assay Kit; Secrete-Pair™ Dual Luminescence Assay Kit]

Liu, H., et al. (2023). Development of novel SARS-CoV-2 viral vectors. Sci Rep doi: 10.1038/s41598-023-40370-8 [Luc-Lysis II Buffer]

Song. M., et al (2023). circIFNGR2 regulating ankylosing spondylitis-associated inflammation through macrophage polarization. iScience doi: 10.1016/j.isci.2023.107325 [miTarget™ 3′ UTR Clone; miTarget™ 3′ UTR miRNA Target Clones; Luc-Pair™ Duo-Luciferase HS Assay Kit]

Wasser, C., et al. (2023). Apoer2-ICD-dependent regulation of hippocampal ribosome mRNA loading. Res Sq doi: 10.21203/rs.3.rs-3040567/v1 [Gluc + SEAP reporter clone pEZX-GA01]

Yang, Y-C., et al. (2023). The RNA-binding protein KSRP aggravates malignant progression of clear cell renal cell carcinoma through transcriptional inhibition and post-transcriptional destabilization of the NEDD4L ubiquitin ligase. J Biomed Sci doi: 10.1186/s12929-023-00949-9 [OMICSLINK™ NEDD4L ORF expression clone; Secrete-Pair™ Gaussia Luciferase Assay kit]

Tsukui, D., et al. (2023). GM-CSF receptor/SYK/JNK/FOXO1/CD11c signaling promotes atherosclerosis. iScience doi: 10.1016/j.isci.2023.107293[OMICSLINK™ Lentiviral expression clone; Secrete-Pair™ Dual Luminescence Assay Kit]

Wischmann, F-J., et al. (2023). Tumor suppressor miR-218 directly targets epidermal growth factor receptor (EGFR) expression in triple-negative breast cancer; sensitizing cells to irradiation. J Cancer Res Clin Oncol doi: 10.1007/s00432-023-04750-x [Secrete-Pair™ Dual Luminescence Assay Kit; miTarget™ 3′ UTR Clone]

Wang, J., et al. (2023). Targeting exosomes enveloped EBV‐miR‐BART1‐5p‐antagomiRs for NPC therapy through both anti‐vasculogenic mimicry and anti‐angiogenesis. Cancer Med doi: 10.1002/cam4.5941 [Luc-Pair™ Duo-Luciferase HS Assay Kit]

Vyas, H., et al. (2023). miR34a-5p impedes CLOCK expression in chronodisruptive C57BL/6J mice and potentiates pro-atherogenic manifestations. PLoS One doi: 10.1371/journal.pone.0283591 [Secrete-Pair™ Dual Luminescence Assay Kit; miTarget™ 3′ UTR Clone]

Wang, Q., et al. (2023). A Novel Circ_Arf3/miR-452-5p/Mbnl1 Axis Regulates Proliferation and Expression of Fibrosis-Related Proteins of Mouse Mesangial Cells Under High Glucose. Diabetes Metab Syndr Obes doi: 10.2147/DMSO.S400530 [Secrete-Pair™ Dual Luminescence Assay Kit]

Jasiecki, J., et al. (2023). Novel Tools for Comprehensive Functional Analysis of LDLR (Low-Density Lipoprotein Receptor) Variants. Int J Mol Sci doi: 10.3390/ijms241411435 [Luc-Pair™ Duo-Luciferase Assay Kit 2.0]

Steadman, K., et al. (2023). Autonomous action and cooperativity between the ONECUT2 transcription factor and its 3′ untranslated region. Front Cell Dev Biol doi: 10.3389/fcell.2023.1206259 [Secrete-Pair™ Dual Luminescence Assay Kit]

Liu, H., et al. (2023). Isorhamnetin and anti-PD-L1 antibody dual-functional mesoporous silica nanoparticles improve tumor immune microenvironment and inhibit YY1-mediated tumor progression. J Nanobiotechnology doi: 10.1186/s12951-023-01967-3 [Secrete-Pair™ Dual Luminescence Assay Kit]

Pan, L., et al. (2023). Osteoclast-Derived Exosomal miR-5134-5p Interferes with Alveolar Bone Homeostasis by Targeting the JAK2/STAT3 Axis. Int J Nanomedicine doi: 10.2147/IJN.S413692 [Luc-Pair™ Duo-Luciferase HT Assay Kit]

Li, H., et al. (2023). FOXO3 regulates Smad3 and Smad7 through SPON1 circular RNA to inhibit idiopathic pulmonary fibrosis. Int J Biol Sci doi: 10.7150/ijbs.80140 [Secrete-Pair™ Dual Luminescence Assay Kit]

Liu, Y., et al. (2023). PTPN2 targets TAK1 for dephosphorylation to improve cellular senescence and promote adipose tissue browning in T2DM. Front Pharmacol doi: 10.3389/fphar.2023.1124633 [Luc-Pair™ Duo-Luciferase Assay Kit 2.0]

Yang, W., et al. (2023). MiR-363 restrain the proliferation; migration and invasion of colorectal carcinoma cell by targeting E2F3. J Cancer doi: 10.7150/jca.83897 [Luc-Pair™ Duo-Luciferase Assay Kit 2.0]

AN, N., et al. (2023). The zinc figure protein ZNF575 impairs colorectal cancer growth via promoting p53 transcription. Oncol Res doi: 10.32604/or.2023.028564 [Luc-Pair™ Duo-Luciferase HS Assay Kit]

Kalantzakos, T., et al. (2023). MicroRNA-155-5p Targets JADE-1; Promoting Proliferation; Migration; and Invasion in Clear Cell Renal Cell Carcinoma Cells. Int J Mol Sci doi: 10.3390/ijms24097825 [miArrest™ miRNA inhibitors; EndoFectin™ Lenti; Luc-Pair™ Duo-Luciferase Assay Kits 2.0]

Sun, B., et al. (2023). Engineered induced-pluripotent stem cell derived monocyte extracellular vesicles alter inflammation in HIV humanized mice. Extracell Vesicles Circ Nucl Acids doi: 10.20517/evcna.2022.11 [miTarget™ 3′ UTR Clone; Secrete-Pair™ Dual Luminescence Assay Kit ]

Zhang, J., et al. (2023). Naringenin in Si-Ni-San formula inhibits chronic psychological stress-induced breast cancer growth and metastasis by modulating estrogen metabolism through FXR/EST pathway. J Adv Res doi: 10.1016/j.jare.2022.06.006 [GLuc-ON™ promoter-reporter clone; Secrete-Pair™ Dual Luminescence Assay Kit]

Dykxhoorn, D.M., et al. (2023). MicroRNA-423-5p Mediates Cocaine-Induced Smooth Muscle Cell Contraction by Targeting Cacna2d2. Int J Mol Sci doi: 10.3390/ijms24076584 [pEZX-MT05 vector; miTarget™ Cacna2d2 3′ UTR Clone; Secrete-Pair™ Dual Luminescence Assay Kit]

Cui, X., et al. (2023). Hepatocellular carcinoma-derived FOXO1 inhibits tumor progression by suppressing IL-6 secretion from macrophages. Neoplasia doi: 10.1016/j.neo.2023.100900 [GLuc-ON™ promoter-reporter clone; Secrete-Pair™ Gaussia Luciferase Assay Kit]

Pang, N., et al. (2023). Nicotinamide Adenine Dinucleotide Precursor Suppresses Hepatocellular Cancer Progression in Mice. Nutrients doi: 10.3390/nu15061447 [EX-hLUC-Lv201; Luc-Pair™ Duo-Luciferase Assay Kit 2.0]

Zheng, Y., et al. (2023). Chronic psychological stress promotes breast cancer pre-metastatic niche formation by mobilizing splenic MDSCs via TAM/CXCL1 signaling. J Exp Clin Cancer Res doi: 10.1186/s13046-023-02696-z [GLuc-ON™ CXCL1 promoter-reporter clone; Luc-Pair™ Duo-Luciferase Assay Kit 2.0]

Xu, H-J., et al. (2023). ESE1/AGR2 axis antagonizes TGF‐β‐induced epithelial‐mesenchymal transition in low‐grade pancreatic cancer. Cancer Med doi: 10.1002/cam4.5397 [LucPair™ Duo‐Luciferase Assay Kit]

Salehi, R., et al. (2023). Androgen-induced exosomal miR-379-5p release determines granulosa cell fate: cellular mechanism involved in polycystic ovaries. J Ovarian Res doi: 10.1186/s13048-023-01141-1 [GLuc-ON™ PDK1 promoter-reporter clone; Luc-Pair™ Duo-Luciferase Assay Kits 2.0]

Shen, D., et al. (2023). Melatonin inhibits bladder tumorigenesis by suppressing PPARγ/ENO1-mediated glycolysis. Cell Death Dis doi: 10.1038/s41419-023-05770-8 [pGL3-ENO1-WT and pGL3-ENO1-Mut luciferase reporter plasmids; Luc-Pair™ Duo-Luciferase Assay Kits 2.0]

Li, Q., et al. (2023). MiR146a-loaded engineered exosomes released from silk fibroin patch promote diabetic wound healing by targeting IRAK1. Signal Transduct Target Ther doi: 10.1038/s41392-022-01263-w [Secrete-Pair™ Gaussia Luciferase Assay Kit]

Cheng, W., et al. (2023). lncRNA TPT1-AS1 promotes cell migration and invasion in esophageal squamous-cell carcinomas by regulating the miR-26a/HMGA1 axis. Open Med (Wars) doi: 10.1515/med-2022-0533 [Luc-Pair™ Duo-Luciferase Assay Kit 2.0]

Zhang, L., et al. (2023). Cytoplasmic Tail Truncation Stabilizes S1-S2 Association and Enhances S Protein Incorporation into SARS-CoV-2 Pseudovirions. J Virol doi: 10.1128/jvi.01650-22 [Luc-Pair™ Firefly Luciferase HS Assay Kit]

Liu, S., et al. (2023). C/EBPβ Coupled with E2F2 Promoted the Proliferation of hESC-Derived Hepatocytes through Direct Binding to the Promoter Regions of Cell-Cycle-Related Genes. Cells doi: 10.3390/cells12030497 [Luc-Pair™ Duo-Luciferase HS Assay Kit]

Li, F., et al. (2023). Reanalysis of ribosome profiling datasets reveals a function of rocaglamide A in perturbing the dynamics of translation elongation via eIF4A. Nat Commun doi: 10.1038/s41467-023-36290-w [miTarget™ miTarget™ 3′ UTR Clone]

Ke, H., et al. (2023). Landscape of pathogenic mutations in premature ovarian insufficiency. Nat Med doi: 10.1038/s41591-022-02194-3 [Secrete-Pair™ Dual Luminescence Assay Kit]

Mongiorgi, S., et al. (2023). A miRNA screening identifies miR-192-5p as associated with response to azacitidine and lenalidomide therapy in myelodysplastic syndromes. Clin Epigenetics doi: 10.1186/s13148-023-01441-9 [miTarget™ BCL2-3′ UTR miRNA Target Clones、Luc-Pair™ Duo-Luciferase Assay Kit 2.0]

LeDesma, R., et al. (2023). Structural features stabilized by divalent cation coordination within hepatitis E virus ORF1 are critical for viral replication. eLife doi: 10.7554/eLife.80529 [Luc-Pair™ Renilla Luciferase HS Assay Kit]

Takashi, M.Y., et al. (2023). Pyrolyzed deketene curcumin controls regulatory T cell generation and gastric cancer metabolism cooperate with 2-deoxy-d-glucose. Front Immunol doi: 10.3389/fimmu.2023.1049713 [Luc-Pair™ Duo-Luciferase Assay Kit 2.0]

Kuang, Y., et al. (2023). The developmental regulator HAND1 inhibits gastric carcinogenesis through enhancing ER stress apoptosis via targeting CHOP and BAK which is augmented by cisplatin. Int J Biol Sci doi: 10.7150/ijbs.76345 [Secrete-Pair™ Dual Luminescence Assay Kit]

Han, X., et al. (2023). miR-1275 targets MDK/AKT signaling to inhibit breast cancer chemoresistance by lessening the properties of cancer stem cells. Int J Biol Sci doi: 10.7150/ijbs.74227 [Luc-Pair™ Duo-Luciferase HS Assay Kit]

Riera, E., et al. (2023). African swine fever virus ubiquitin-conjugating enzyme pI215L inhibits IFN-I signaling pathway through STAT2 degradation. Front Microbiol doi: 10.3389/fmicb.2022.1081035 [Luc-Pair™ Duo-Luciferase HS Assay Kit]

Palakurti, R., et al. (2023). Inducible miR-1224 silences cerebrovascular Serpine1 and restores blood flow to the stroke-affected site of the brain. Mol Ther Nucleic Acids doi: 10.1016/j.omtn.2022.12.019 [Secrete-Pair™ Dual Luminescence Assay Kit]

Becker, V., et al. (2023). Hypoxia-induced downregulation of microRNA-186-5p in endothelial cells promotes non-small cell lung cancer angiogenesis by upregulating protein kinase C alpha. Mol Ther Nucleic Acids doi: 10.1016/j.omtn.2023.01.015 [Luc-Pair™ Duo-Luciferase HS Assay Kit]

Zheng, Z., et al. (2023). Mir155 regulates osteogenesis and bone mass phenotype via targeting S1pr1 gene. eLife doi: 10.7554/eLife.77742 [Luc-Pair™ Duo-Luciferase Assay Kit 2.0]

Li, Z., et al. (2023). Circ_0020256 induces fibroblast activation to drive cholangiocarcinoma development via recruitment of EIF4A3 protein to stabilize KLF4 mRNA. Cell Death Discov doi: 10.1038/s41420-023-01439-5 [All-in-One™ First-Strand cDNA Synthesis Kit; All-in-One™ qPCR Mix]

Mao,Y., et al. (2023). Identification Invasion-Related Long Non-Coding RNAs in Lung Adenocarcinoma and Analysis of Competitive Endogenous RNA Regulatory Networks. Int J Gen Med doi: 10.2147/IJGM.S407266 [SureScript™ First-Strand cDNA Synthesis Kit; BlazeTaq™ SYBR® Green qPCR mix 2.0]